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Method of simultaneously extracting theanine, rubusoside, and tea polyphenol from sweet tea

A technology of theanine and rubusoside, which is applied in the fields of extracting theanine from sweet tea and simultaneously extracting rubusoside and tea polyphenols, which can solve the problems of low content and yield of rubusoside and polyphenols

Active Publication Date: 2018-11-06
HUNAN HUACHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the content and yield of rubusoside and polyphenols obtained by this method are all on the low side

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0094] The method of extracting theanine from sweet tea:

[0095] (1) Percolation: crush 100kg of dry sweet tea leaves to 60 mesh, place them in a percolation device, and use 500kg of cold water at 20°C to percolate at a flow rate of 0.5BV / h to obtain percolation liquid;

[0096] (2) Macroporous adsorption resin column adsorption: pass the percolation liquid obtained in step (1) through the LX-T28 macroporous adsorption resin column at a flow rate of 0.5BV / h (the amount of resin used is 50L, and the height-to-diameter ratio of the resin column is 4:1) Adsorb on the column and collect the effluent;

[0097] (3) Polyamide resin column adsorption: Pass the effluent obtained in step (2) at a flow rate of 0.5BV / h through a polyamide resin column (the amount of resin used is 40L, and the height-to-diameter ratio of the resin column is 3:1) for adsorption on the column , to collect the effluent;

[0098] (4) Extraction of theanine: pass the effluent obtained in step (3) through a 001...

Embodiment 2

[0107] The method of extracting theanine from sweet tea:

[0108] (1) Percolation: Grind 200kg of dry sweet tea leaves to 40 meshes, place them in a percolation device, and use 1600kg of cold water at 15°C to percolate at a flow rate of 0.8BV / h to obtain percolation liquid;

[0109] (2) Macroporous adsorption resin column adsorption: pass the percolation liquid obtained in step (1) through the AB-8 macroporous adsorption resin column at a flow rate of 1BV / h (the amount of resin used is 120L, and the height-to-diameter ratio of the resin column is 2 :1) Adsorb on the column and collect the effluent;

[0110] (3) Polyamide resin column adsorption: Pass the effluent obtained in step (2) at a flow rate of 1.5BV / h through a polyamide resin column (the amount of resin used is 60L, and the height-to-diameter ratio of the resin column is 4:1) for adsorption on the column , to collect the effluent;

[0111] (4) Extraction of theanine: Pass the effluent obtained in step (3) at a flow ...

Embodiment 3

[0120] The method of extracting theanine from sweet tea:

[0121] (1) Percolation: crush 100kg of dry sweet tea leaves to 40 meshes, place them in a percolation device, and use 800kg of cold water at 25°C to percolate at a flow rate of 1BV / h to obtain percolation liquid;

[0122] (2) Macroporous adsorption resin column adsorption: pass the percolation liquid obtained in step (1) through the XAD-16 macroporous adsorption resin column at a flow rate of 1.5BV / h (the amount of resin used is 40L, and the height-to-diameter ratio of the resin column is 3:1) Adsorb on the column and collect the effluent;

[0123] (3) Polyamide resin column adsorption: The effluent obtained in step (2) is passed through a polyamide resin column (the amount of resin used is 50L, and the height-to-diameter ratio of the resin column is 2:1) at a flow rate of 1BV / h for adsorption on the column. Collect effluent;

[0124] (4) Extraction of theanine: pass the effluent obtained in step (3) through the LX-T...

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PUM

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Abstract

The invention discloses a method of simultaneously extracting theanine, rubusoside, and tea polyphenol from sweet tea. The method comprises following steps: (1) grinding sweet tea, and percolating sweet tea by cold water to obtain percolate; (2) adsorbing percolate by a macroporous resin column, and collecting the eluent; (3) adsorbing the eluent by a polyamide resin column and collecting the eluent; (4) making the eluent go through an ion exchange resin column, washing the ion exchange resin column by water, then eluting the ion exchange resin column by a brine solution, concentrating the eluent, cooling, adjusting the pH to the acidic range, stirring the solution to carry out crystallization, filtering the solution, washing crystals, and drying to obtain theanine. The invention also discloses a method of simultaneously extracting rubusoside, and tea polyphenol. The contents of obtained theanine, rubusoside, and tea polyphenol can reach 98.1%, 99.9%, and 98.3%; the yields can reach 95%, 97%, and 92%; the sweet tea resources are comprehensively utilized, the maneuverability is strong, the cost is low, the sewage discharge is little, and the method is suitable for industrial production.

Description

technical field [0001] The invention relates to a method for extracting theanine from sweet tea and simultaneously extracting multiple active ingredients, in particular to a method for extracting theanine from sweet tea and simultaneously extracting rubusoside and tea polyphenols. Background technique [0002] Sweet tea (scientific name Rubus Suavissimus S.Lee, English name Sweet tea), also known as Rubus sweet leaf, is a perennial thorny shrub of the genus Rubus in the family Rosaceae. It was discovered as a drug in my country in the early 1980s. Sweet tea is sweet and refreshing, not only can produce body fluids and quench thirst, but also has good pharmacological effects and health care functions on the human body. According to the records of "Chinese Medicine" and related literature reports: "Sweet tea can prevent and treat high blood pressure, treat damp-heat dysentery, skin itching, carbuncle and malignant sores, and has the functions of nourishing the liver and kidney...

Claims

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Application Information

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IPC IPC(8): C07C231/24C07C237/06C07H15/256C07H1/08C07G99/00
CPCC07C231/24C07H1/08C07H15/256C07G99/00C07C237/06
Inventor 李伟龙伟岸黄华学
Owner HUNAN HUACHENG BIOTECH
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