Method used for synchronous preparation of euphausia superba oil and protein
An Antarctic krill oil and Antarctic krill technology, which is applied in the field of marine biological resource utilization, can solve the problems of limiting the processing efficiency of Antarctic krill, product value, poor functional and application characteristics, and poor quality of by-products, etc. The effect of good oil and protein quality, improved industrial value, and improved separation efficiency
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Embodiment 1
[0020] A preparation method for synchronously obtaining Antarctic krill oil and protein, comprising the following steps:
[0021] Step 1. Using a wet pulverizer to crush the Antarctic krill, with the average particle size controlled at 100 μm, to obtain crushed Antarctic krill.
[0022] Step 2: Add 1% (wt) Tween 20 solution to the obtained crushed Antarctic krill, and mix thoroughly; use NaOH solution to adjust the pH of the system to 9.0, and leaching for 10 minutes at a temperature of 30° C. to obtain an extract.
[0023] Step 3, using a three-phase decanter centrifuge at 20,000 rpm to separate the obtained extract for 10 minutes to obtain an oil phase, an aqueous phase, and a residue respectively;
[0024] Step 4. Molecular distillation of the oil phase (distillation temperature 120° C., feed rate 1.0 L / h, film scraping speed 400 r / min) to obtain Antarctic krill oil.
[0025] Step 5. Adjust the pH of the aqueous phase to 4.0 with hydrochloric acid solution, and slowly stir...
Embodiment 2
[0029] A preparation method for synchronously obtaining Antarctic krill oil and protein, comprising the following steps:
[0030] Step 1. Using a wet pulverizer to crush the Antarctic krill, with the average particle size controlled at 500 μm, to obtain crushed Antarctic krill.
[0031] Step 2: Add 10% (wt) Tween 20 solution to the obtained crushed Antarctic krill, and mix thoroughly; use NaOH solution to adjust the pH of the system to 11.0, and leaching for 50 minutes at a temperature of 60° C. to obtain an extract.
[0032] Step 3, using a three-phase decanter centrifuge at 10,000 rpm to separate the obtained extract for 60 minutes to obtain an oil phase, an aqueous phase, and a residue respectively;
[0033] Step 4: Molecular distillation of the oil phase (distillation temperature 300° C., feed rate 0.75 L / h, film scraping speed 300 r / min) to obtain Antarctic krill oil.
[0034] Step 5. Adjust the pH of the aqueous phase to 5.0 with hydrochloric acid solution, and slowly s...
Embodiment 3
[0038] A preparation method for synchronously obtaining Antarctic krill oil and protein, comprising the following steps:
[0039] Step 1. Using a wet pulverizer to crush the Antarctic krill, with the average particle size controlled at 300 μm, to obtain crushed Antarctic krill.
[0040] Step 2: Add 5% (wt) Tween 20 solution to the obtained crushed Antarctic krill, and mix thoroughly; use NaOH solution to adjust the pH of the system to 10.0, and extract at 45° C. for 30 minutes to obtain an extract.
[0041] Step 3, using a three-phase decanter centrifuge at 12,000 rpm to separate the obtained extract for 30 minutes to obtain an oil phase, an aqueous phase, and a residue;
[0042] Step 4. Molecular distillation of the oil phase (distillation temperature 320° C., feed rate 0.01 L / h, film scraping speed 40 r / min) to obtain Antarctic krill oil.
[0043] Step 5. Adjust the pH of the aqueous phase to 4.5 with hydrochloric acid solution, and slowly stir for 30 minutes; continue to u...
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