Separation analysis method of derivatized heparin sulfate disaccharides containing free amino groups

A technology of heparan sulfate and free amino groups, which is applied in the field of separation and analysis of heparan sulfate disaccharides, can solve the problems of mass spectrometry sensitivity decrease and ion source contamination of mass spectrometry, and achieve the expansion of instrument application range, reduce instrument cost, and maximize practical value Effect
CN108802248AActive Publication Date: 2018-11-13FUZHOU UNIV
7 Cites 1 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
FUZHOU UNIV
Publication Date
2018-11-13

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a separation analysis method of derivatized heparin sulfate disaccharides containing free amino groups. The function relationship of the disaccharide quantity and mass spectrumdetection peak area can be used for quantitative determination of heparin and heparin sulfate disaccharide in practical biological samples. The separation analysis method can be used for detection ofheparin and heparin sulfate disaccharides, and especially heparin sulfate disaccharides containing free amino groups in liquid phase-mass spectrometry, and routine liquid chromatogram separating combined ultraviolet and fluorescent detector can be adopted in analysis. Important effect on clinical study on medical heparin, heparin sulfate, heparin derivatives, and disaccharides in various biological samples is achieved.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the technical field of drug detection, and in particular relates to a method for separating and analyzing derivatized heparan sulfate disaccharides containing free amino groups. Background technique

[0002] Heparin and heparan sulfate are linear glycosaminoglycans. Because heparin has a strong anticoagulant effect, it is widely used in the treatment of thromboembolic diseases, myocardial infarction and other diseases. Different sulfation, acetylation and free amino substitution patterns in heparin form different disaccharide units, endowing heparin and heparan sulfate with numerous biological functions, participating in angiogenesis, blood coagulation, cell adhesion, proliferation and tumor metastasis and other life activities. Heparin and heparan sulfate are composed of repeating disaccharide units composed of hexuronic acid and glucosamine connected by 1-4 glycosidic bonds, of which the conventional N-sulfate is present in ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More