Thin-layer identification method used for controlling qualities of dry leaves of Nelumbo nucifera Gaertn, decoction pieces and formula granules

A technology for lotus leaf and medicinal materials, which is applied in the field of thin-layer identification used for quality control of lotus leaf medicinal materials, decoction pieces and formula granules, can solve the problem that many influencing factors cannot be used for identifying lotus leaf extract formula granules, formula preparations, Increase uncontrollable factors and other issues to achieve the effect of high accuracy, good application prospects, and good practical value

Active Publication Date: 2018-11-13
SICHUAN NEO GREEN PHARMA TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The microscopic identification method stipulated in the above Pharmacopoeia is only applicable to the identification of peppermint raw powder, and cannot be used to identify lotus leaf extract and its formulated granules and prescription preparations.
The thin-layer method contained in the above-mentioned invention patent: first, the preparation steps of the test solution are relatively complicated, and there are many influencing factors; It can already be reflected in the chromatogram of the reference medicinal material, and more materials need to be prepared; the third is that the expansion cylinder needs to be pre-saturated with ammonia vapor before expansion, which is more cumbersome to operate and also increases uncontrollable factors

Method used

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  • Thin-layer identification method used for controlling qualities of dry leaves of Nelumbo nucifera Gaertn, decoction pieces and formula granules
  • Thin-layer identification method used for controlling qualities of dry leaves of Nelumbo nucifera Gaertn, decoction pieces and formula granules
  • Thin-layer identification method used for controlling qualities of dry leaves of Nelumbo nucifera Gaertn, decoction pieces and formula granules

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1 Detection method of the present invention

[0038] 1. Detection method

[0039] (1) Preparation of the test solution: take the lotus leaf medicinal material and pulverize it into fine powder, mix well, take 1 g of the fine powder, add 50 ml of water, boil for 30 minutes, filter, evaporate the filtrate to dryness, add 2 ml of concentrated ammonia test solution to the residue to moisten Wet, add 30ml of dichloromethane, ultrasonic treatment for 30 minutes, filter, evaporate to dryness, add methanol to dissolve the residue, as the test solution.

[0040] (2) Preparation of the reference medicinal material solution: take 1 g of genuine lotus leaf reference medicinal material powder, and prepare it according to the same method as step (1), as the reference medicinal material solution.

[0041] (3) Thin-layer chromatography identification method: According to the thin-layer chromatography test of the fourth general rule 0502 of the "Chinese Pharmacopoeia" 2015 edi...

Embodiment 2

[0042] Embodiment 2 detection method of the present invention

[0043] 1. Detection method

[0044] (1) Preparation of the test solution: Take lotus leaf decoction pieces and crush them into fine powder, mix well, take 1g of the fine powder, add 30ml of water, boil for 30 minutes, filter, evaporate the filtrate to dryness, add 1ml of concentrated ammonia test solution to the residue to moisten Wet, add 20ml of dichloromethane, ultrasonic treatment for 15 minutes, filter, evaporate to dryness, add methanol to dissolve the residue, as the test solution.

[0045] (2) Preparation of the reference medicinal material solution: take 1 g of genuine lotus leaf reference medicinal material powder, and prepare it according to the same method as step (1), as the reference medicinal material solution.

[0046] (3) Thin-layer chromatography identification method: According to the thin-layer chromatography test of the fourth general rule 0502 of the "Chinese Pharmacopoeia" 2015 edition, dra...

Embodiment 3

[0047] Embodiment 3 detection method of the present invention

[0048] 1. Detection method

[0049] (1) Preparation of the test solution: get the lotus leaf formula granules and grind them into fine powder, mix evenly, get the fine powder 1g (according to lotus leaf decoction pieces), add 80ml of water, boil for 15 minutes, filter, and the filtrate is evaporated to dryness. Wet the residue with 2ml of concentrated ammonia test solution, add 45ml of dichloromethane, ultrasonicate for 45 minutes, filter, evaporate to dryness, dissolve the residue with methanol, and use it as the test solution.

[0050] (2) Preparation of the reference medicinal material solution: take 1 g of genuine lotus leaf reference medicinal material powder, and prepare it according to the same method as step (1), as the reference medicinal material solution.

[0051] (3) Thin-layer chromatography identification method: According to the thin-layer chromatography test of the fourth general rule 0502 of the ...

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Abstract

The invention discloses a thin-layer identification method used for controlling qualities of dry leaves of Nelumbo nucifera Gaertn, decoction pieces and formula granules. The thin-layer identificationmethod is mainly characterized in that an Rf (retardation factor) ranging from 0.3 to 0.7 represents spots, and the detecting condition is that a stationary phase adopts a silica G thin-layer plate;a developing solvent is composed of petroleum ether (60 to 90 DEG C), dichloromethane, acetone and diethylamine with the volume ratio of 7-9 to 1-3 to 0.5-1.5 to 1-2. The thin-layer identification method provided by the invention not only can be used for controlling the qualities of dry leaves of Nelumbo nucifera Gaertn and the decoction pieces, but also can be used for controlling qualities of formula granule intermediates and finished products of the dry leaves of Nelumbo nucifera Gaertn, the detecting method is high in accuracy, stable and feasible, good in adaptation, convenient to operateand low in cost and has a good application prospect.

Description

technical field [0001] The invention relates to a TLC identification method for quality control of lotus leaf medicinal materials, decoction pieces and formula granules. Background technique [0002] The lotus leaf is the dried leaf of Nelumbo nucifera Gaertn., a plant of the water lily family. It was first recorded in "Diet Therapy Materia Medica". , Diarrhea due to spleen deficiency, spit mandrill due to blood heat, bloody stool and metrorrhagia, are commonly used clinical traditional Chinese medicine varieties. The lotus leaf mainly contains various alkaloid components such as nuciferine, protonuciferine, and pronuciferine, as well as flavonoids such as quercetin. Modern studies have shown that alkaloids in lotus leaves have pharmacological activities such as lipid-lowering, weight-loss, antibacterial, and antiviral, and are the main active ingredients of lotus leaves. [0003] In order to ensure the quality of the lotus leaf, it needs to be quality controlled. At pres...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90
CPCG01N30/90
Inventor 周厚成胡昌江李文兵邢冷姜艳娇喻荣平
Owner SICHUAN NEO GREEN PHARMA TECH DEV
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