Thin-layer identification method used for controlling qualities of dry leaves of Nelumbo nucifera Gaertn, decoction pieces and formula granules

Abstract

The invention discloses a thin-layer identification method used for controlling qualities of dry leaves of Nelumbo nucifera Gaertn, decoction pieces and formula granules. The thin-layer identificationmethod is mainly characterized in that an Rf (retardation factor) ranging from 0.3 to 0.7 represents spots, and the detecting condition is that a stationary phase adopts a silica G thin-layer plate;a developing solvent is composed of petroleum ether (60 to 90 DEG C), dichloromethane, acetone and diethylamine with the volume ratio of 7-9 to 1-3 to 0.5-1.5 to 1-2. The thin-layer identification method provided by the invention not only can be used for controlling the qualities of dry leaves of Nelumbo nucifera Gaertn and the decoction pieces, but also can be used for controlling qualities of formula granule intermediates and finished products of the dry leaves of Nelumbo nucifera Gaertn, the detecting method is high in accuracy, stable and feasible, good in adaptation, convenient to operateand low in cost and has a good application prospect.

Description

technical field [0001] The invention relates to a TLC identification method for quality control of lotus leaf medicinal materials, decoction pieces and formula granules. Background technique [0002] The lotus leaf is the dried leaf of Nelumbo nucifera Gaertn., a plant of the water lily family. It was first recorded in "Diet Therapy Materia Medica". , Diarrhea due to spleen deficiency, spit mandrill due to blood heat, bloody stool and metrorrhagia, are commonly used clinical traditional Chinese medicine varieties. The lotus leaf mainly contains various alkaloid components such as nuciferine, protonuciferine, and pronuciferine, as well as flavonoids such as quercetin. Modern studies have shown that alkaloids in lotus leaves have pharmacological activities such as lipid-lowering, weight-loss, antibacterial, and antiviral, and are the main active ingredients of lotus leaves. [0003] In order to ensure the quality of the lotus leaf, it needs to be quality controlled. At pres...

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Problems solved by technology

[0009] The microscopic identification method stipulated in the above Pharmacopoeia is only applicable to the identification of peppermint raw powder, and cannot be used to identify lotus leaf extract and its formulated granules and prescription preparations.

The thin-layer method contained in the above-mentioned invention patent: first, the preparation steps of the test solution are relatively complicated, and there are many influencing factors; It can already be reflected in the chromatogram of the reference medicinal material, and more materials need to be prepared; the third is that the expansion cylinder needs to be pre-saturated with ammonia vapor before expansion, which is more cumbersome to operate and also increases uncontrollable factors

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