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Cod skin collagen peptide with biological activity

A collagen peptide, bioactive technology, applied in animal/human proteins, microorganism-based methods, microorganisms, etc., can solve problems such as environmental pollution, resource waste, etc., achieve high solubility, strong scavenging ability, improve yield and quality effect

Inactive Publication Date: 2018-11-16
兰溪市沉默生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the rapid development of the aquatic product processing industry, a large amount of fish leftovers, such as fish skin, fish scales, viscera, fish heads, etc., will be produced. Most of the feed is discarded, which not only causes a great waste of resources, but also causes environmental pollution

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  • Cod skin collagen peptide with biological activity

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Effect test

Embodiment 1

[0017] A biologically active cod skin collagen peptide. Among the collagen peptides, peptides with a molecular weight of ≤3kDa account for more than 90% of the total collagen peptides. The smaller the molecular weight of the collagen peptide, the stronger its ability to scavenge DPPH free radicals, hydroxyl free radicals and superoxide anions, and the scavenging effect of the collagen peptide on free radicals is much better than that of V C , has a good application prospect.

[0018] A preparation method of biologically active cod skin collagen peptides, specifically comprising the steps of:

[0019] 1) Culture medium preparation: steam the fish skin, add water and arabinose to homogenate, the solid-to-liquid ratio of fish skin and water in the homogenate is 1:15, and sterilize to obtain the fermentation medium;

[0020] 2) Preparation of seed liquid: activate the mixed strains, inoculate them in liquid medium, and culture them on a shaking table at 35°C and 250r / min for 30 h...

Embodiment 2

[0026] A biologically active cod skin collagen peptide. Among the collagen peptides, peptides with a molecular weight of ≤3kDa account for more than 90% of the total collagen peptides.

[0027] A preparation method of biologically active cod skin collagen peptides, specifically comprising the steps of:

[0028] 1) Culture medium preparation: steam the fish skin, add water and arabinose to homogenate, the solid-to-liquid ratio of fish skin and water in the homogenate is 1:12, and sterilize to obtain the fermentation medium;

[0029] 2) Preparation of seed liquid: activate the mixed strains, inoculate them in liquid medium, and culture them on a shaking table at 37°C and 200r / min for 36 hours to obtain seed liquid for later use;

[0030] 3) Fermentation: Put the seed liquid into the fermentation medium according to the inoculum amount of 8%, cultivate it at 30°C and 200r / min for 24 hours, concentrate the supernatant by centrifugation, and then add β-cyclodextrin with 5-8% weight...

Embodiment 3

[0033] A biologically active cod skin collagen peptide. Among the collagen peptides, peptides with a molecular weight of ≤3kDa account for more than 90% of the total collagen peptides.

[0034] A preparation method of biologically active cod skin collagen peptide, specifically comprising the steps of:

[0035] 1) Culture medium preparation: steam the fish skin, add water and arabinose to homogenate, the solid-to-liquid ratio of fish skin and water in the homogenate is 1:10, and sterilize to obtain the fermentation medium;

[0036] 2) Preparation of seed solution: activate the mixed strains, inoculate them in liquid medium, and culture them on a shaking table at 40°C and 150r / min for 40 hours to obtain seed solution, which is set aside;

[0037] 3) Fermentation: Put the seed liquid into the fermentation medium according to the inoculum amount of 7%, culture at 35°C and 150r / min for 25h, centrifuge the supernatant to concentrate, then add β-cyclodextrin with 5% weight of fish skin...

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Abstract

The invention discloses a cod skin collagen peptide with biological activity. Peptides with a molecular weight of <= 3kDa in the collagen peptide account for 90% or more of the total collagen peptide.A preparation method of the collagen peptide comprises the following steps: cooking fish skin, adding water and arabinose homogenate, and performing sterilization to obtain a fermentation culture medium; activating a mixed strain, performing inoculation in a liquid culture medium, and performing cultivation to obtain a seed liquid for standby application; and performing inoculation by using the seed liquid in the fermentation culture medium, performing cultivation, performing centrifugation, performing concentration on the supernatant, adding beta-cyclodextrin, performing ultra-high-pressuretreatment, performing filtering, and performing freeze drying on the filtrate, so as to obtain the collagen peptide. The collagen peptide disclosed by the invention has the beneficial effects that thecollagen peptide has a smaller molecular weight, strong removing effects and ability on DPPH free radicals, hydroxyl free radicals and superoxide anions, no a bitter or fishy taste, and uniform molecular weight distribution, and is the cod skin collagen peptide with the biological activity and higher sensory acceptability, yield and quality.

Description

technical field [0001] The invention relates to the technical field of bioactive ingredients, in particular to a cod skin collagen peptide with bioactivity. Background technique [0002] In recent years, with the improvement of living standards, the diversity and practicability of people's demand for aquatic resources have gradually increased, thus promoting the rapid development of my country's aquatic product processing industry. However, with the rapid development of the aquatic product processing industry, a large amount of fish leftovers, such as fish skin, fish scales, viscera, fish heads, etc., will be produced. Most of the feed is discarded, which not only causes a great waste of resources, but also causes environmental pollution. Therefore, how to efficiently utilize the nutritionally active substances in fish scraps has attracted great attention from scientific researchers at home and abroad. Among them, the main waste fish skin is rich in collagen, and collagen c...

Claims

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Application Information

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IPC IPC(8): C07K14/78C12P21/02C12R1/125C12R1/645
CPCC07K14/78C12P21/02
Inventor 周建波
Owner 兰溪市沉默生物科技有限公司
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