The invention discloses deer blood wine and a preparation method of the deer blood wine and belongs to the technical field of nutritional Baijiu. Fresh deer blood and deer whips are used as main raw materials and are scientifically compounded with lycium ruthenicum, fructus lycii, mulberry fruit, fructus jujubae, longan, liquorice root and the like; a whole process adopts low-temperature processing technologies of freeze-drying, carrying out biological enzymolysis by a complex enzyme, degassing and deodorizing in vacuum, freezing, carrying out low-temperature fermentation by functional saccharomyces cerevisiae, carrying out ultrasonic processing, carrying out high-voltage pulse electric field processing and the like; the utilization rate of effective components of deer products is effectively improved, and the loss of nutrients and health-care substances is reduced; the original color and luster, flavor and the content of bioactive components and nutrient substances of the deer products are kept to the greatest extent. Finally, the deer blood wine which has no odor, high stability, coordinated taste, mellowness, softness, long aftertaste, moderate flavor, high bioactive components and nutrient value and good health-care effect and is clarified and transparent is prepared.