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Carnivore Aleutian virus and feline panleukocytic parvovirus two-way direct PCR detection reagent and detection kit and application

A detection kit, Aleutian virus technology, applied in biochemical equipment and methods, microbial determination/inspection, microorganisms, etc., can solve problems such as easy cross-contamination, lower virus detection rate, easy loss of target DNA, etc. Highly targeted, conducive to virus detection, fast and accurate virus detection effect

Active Publication Date: 2021-10-26
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is easy to cross-contaminate when extracting nucleic acid samples from batch samples, and the target DNA is easy to be lost during the extraction of trace samples, which reduces the detection rate of viruses

Method used

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  • Carnivore Aleutian virus and feline panleukocytic parvovirus two-way direct PCR detection reagent and detection kit and application
  • Carnivore Aleutian virus and feline panleukocytic parvovirus two-way direct PCR detection reagent and detection kit and application

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Effect test

Embodiment 1

[0047] This embodiment provides a detection reagent for virus detection, the detection reagent includes a detection primer combination of animal-derived components; the detection primer combination includes the first primer pair for detecting Aleutian virus and the second primer pair for detecting feline panleukocytic parvovirus; the first primer The base sequences of the pair are respectively shown in SEQ ID No.1-2, and the base sequences of the second primer pair are respectively shown in SEQ ID No.3-4.

[0048] The first primer pair for detection of Aleutian virus:

[0049] The upstream primer SEQ ID NO.1 is AVF:5-ccaacaagtMatgacaccttggt-3';

[0050] The downstream primer SEQ ID NO.2 is AVR:5'-gttggtttggttgctctccaagga-3';

[0051] The product length is 316bp.

[0052] The second primer pair for detecting feline panleukocytic parvovirus:

[0053] The upstream primer SEQ ID NO.3 is ParF: 5'-tgcacaaattgtaacaccttggtc-3';

[0054] The downstream primer SEQ ID NO.4 is ParR:5'...

Embodiment 2

[0059] This embodiment provides a detection kit for virus detection, the detection kit includes a detection primer combination of animal-derived components; the detection primer combination includes the first primer pair for detection of Aleutian virus and the second primer pair for detection of feline panleukoparvovirus; The base sequences of the first primer pair are respectively shown in SEQ ID No.1-2, and the base sequences of the second primer pair are respectively shown in SEQ ID No.3-4.

Embodiment 3

[0061] This embodiment provides a detection kit for virus detection, the detection kit includes a detection primer combination of animal-derived components; the detection primer combination includes the first primer pair for detection of Aleutian virus and the second primer pair for detection of feline panleukoparvovirus; The base sequences of the first primer pair are respectively shown in SEQ ID No.1-2, and the base sequences of the second primer pair are respectively shown in SEQ ID No.3-4.

[0062] Of course, the above detection kit may also include at least one of nucleic acid releasing agent, PCR premix buffer, DNA polymerase and pMD18-T+ADV-VP2 plasmid positive template. The PCR premix buffer is a 2× solution, which is convenient for experimental operation.

[0063] Nucleic acid release agents include 0.4M KOH and 0.1% Triton X-100.

[0064] 2×PCR master mix buffer includes 20mM Tris-HCl, the pH value of Tris-HCl is 7.0; 8mM KCl, 4mM MgCl 2 and 0.4mM dNTPs; and 2mL / L ...

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Abstract

The invention provides a dual direct PCR detection reagent for carnivore Aleutian virus and feline panleukocytic parvovirus, a detection kit and an application thereof, belonging to the field of biological inspection and quarantine. The detection reagent for virus detection provided by the invention has strong pertinence and high specificity, and can conveniently and rapidly detect virus components in samples. Applying the above-mentioned detection reagents for virus detection to the detection of virus components in samples, the detection results are more accurate and reliable; applied to the preparation of detection kits for detection of virus components in samples, the virus can be detected more quickly and accurately, and the detection results are highly reliable and have a relatively high reliability. High reagent application value and promotion value.

Description

technical field [0001] The invention relates to the field of biological inspection and quarantine, in particular to a dual direct PCR detection reagent for carnivore Aleutian virus and feline panleukocytic parvovirus, a detection kit and its application. Background technique [0002] Animals Aleutian virus infects mustelidae, skunks, and red pandas. It is the causative agent of Aleutian disease in mink, ferret, otter, skunk, raccoon dog, and red panda. Potential pathogens of animals such as raccoons and raccoon dogs. Feline panleukovirus is a parvovirus that causes enteritis in mustelidae, canines, felines and other animals, and is an important pathogen of carnivorous animals. [0003] For the traditional PCR detection of two types of viruses, the conventional path needs to extract viral nucleic acid, which increases the cost of detection and delays the detection time of large samples. It is easy to cross-contaminate when extracting nucleic acid samples from batch samples,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12Q1/686C12R1/93
CPCC12Q1/686C12Q1/701C12Q2600/16C12Q2537/143
Inventor 邵西群章秀婷杨福合
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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