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Method for efficiently producing L-isoleucine

An isoleucine and high-efficiency technology, which is applied in the field of efficient production of L-isoleucine, can solve the problems of genetically engineered bacteria with low forward mutation rate of mutant strains, and achieve the effect of improving acid production capacity.

Active Publication Date: 2018-11-20
WUXI JINGHAI AMINO ACID
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, due to the extremely low forward mutation rate of mutant strains and the uncertainty of the construction of genetically engineered bacteria, it is urgent to find new ways to improve the production of Brevibacterium lactose fermenterum, Corynebacterium blunt-toothed and Bacillus viscosus. Acid Level Ideas

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Brevibacterium lactofermentation bacteria powder was inoculated at an inoculum amount of 0.1g / L in medium A with a liquid content of 40% for strain cultivation. The conditions were temperature 31°C, time 18h, rotation speed 270rpm, ventilation Quantity 4M 3 / h, to obtain the Brevibacterium lactose fermentum strain;

[0050] Beijing Corynebacterium pekinense (Corynebacterium pekinense) bacteria powder was inoculated with an inoculum amount of 0.1g / L in medium B with a liquid content of 40% to carry out strain cultivation, the conditions were temperature 30°C, time 16h, rotation speed 250rpm, to obtain Beijing Corynebacterium species;

[0051] The two obtained strains were inoculated at 0.2g / L and 0.02g / L inoculum amounts respectively in medium C with a liquid content of 40% for fermentation and culture, and the conditions were as follows: temperature 31°C, time 68h, rotation speed 135rpm, ventilation Volume 180M 3 / h to obtain the fermentation broth.

[0052] The fer...

Embodiment 2

[0054] Brevibacterium lactofermentation bacteria powder was inoculated at an inoculum amount of 0.1g / L in medium A with a liquid content of 40% for strain cultivation. The conditions were temperature 31°C, time 18h, rotation speed 270rpm, ventilation Quantity 4M 3 / h, to obtain the Brevibacterium lactose fermentum strain;

[0055] Beijing Corynebacterium pekinense (Corynebacterium pekinense) bacteria powder was inoculated with an inoculum amount of 0.1g / L in medium B with a liquid content of 40% to carry out strain cultivation, the conditions were temperature 30°C, time 16h, rotation speed 250rpm, to obtain Beijing Corynebacterium species;

[0056] The two obtained strains were inoculated with 0.2g / L and 0.01g / L inoculum amounts respectively in medium C with a liquid content of 40% for fermentation and culture. The conditions were temperature 31°C, time 68h, rotation speed 135rpm, ventilation Volume 180M 3 / h to obtain the fermentation broth.

[0057] The fermentation brot...

Embodiment 3

[0059] Brevibacterium lactofermentation bacteria powder was inoculated at an inoculum amount of 0.1g / L in medium A with a liquid content of 40% for strain cultivation. The conditions were temperature 31°C, time 18h, rotation speed 270rpm, ventilation Quantity 4M 3 / h, to obtain the Brevibacterium lactose fermentum strain;

[0060] Beijing Corynebacterium pekinense (Corynebacterium pekinense) bacteria powder was inoculated with an inoculum amount of 0.1g / L in medium B with a liquid content of 40% to carry out strain cultivation, the conditions were temperature 30°C, time 16h, rotation speed 250rpm, to obtain Beijing Corynebacterium species;

[0061] The two obtained strains were inoculated at 0.2g / L and 0.03g / L inoculum amounts respectively in medium C with a liquid content of 40% for fermentation and cultivation, and the conditions were as follows: temperature 31°C, time 68h, rotation speed 135rpm, ventilation Volume 180M 3 / h to obtain the fermentation broth.

[0062] The...

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PUM

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Abstract

The invention discloses a method for efficiently producing L-isoleucine, and belongs to the technical field of microbial fermentation. In the method provided by the invention, brevibacterium lactofermentation is taken as a production strain of L-isoleucine, and the brevibacterium lactofermentation and corynebacterium pekinense are co-cultured, so that the acid production capacity of the brevibacterium lactofermentation is significantly improved from 13g / L to 21g / L, which can meet the needs of industrial production.

Description

technical field [0001] The invention relates to a method for efficiently producing L-isoleucine, which belongs to the technical field of microbial fermentation. Background technique [0002] L-isoleucine is one of the eight essential amino acids for the human body and one of the three branched-chain amino acids. It is the raw material for the synthesis of human hormones and enzymes. It has the effect of promoting protein synthesis and inhibiting its decomposition. It plays an important role in life activities and has been widely used in biomedicine, tissue engineering, photochemistry, electrochemistry, food industry, cosmetics and other fields. In foreign countries, L-isoleucine is also widely used in lactation of dairy cows, preparation of feed additives and production of functional drinks. [0003] At present, microbial fermentation is commonly used to produce L-isoleucine, and in microbial fermentation, strains play a vital role. [0004] Existing L-isoleucine producing...

Claims

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Application Information

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IPC IPC(8): C12P13/06C12R1/13
CPCC12P13/06
Inventor 宁健飞蔡立明陈晓双
Owner WUXI JINGHAI AMINO ACID
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