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Green production method of L-tryptophan by using thallus protease hydrolysate instead of yeast powder

A proteolytic solution and green production technology, applied in the field of L-tryptophan production, to achieve the effects of reducing industrial costs, mild processing conditions and high protein titer

Active Publication Date: 2019-04-12
XINJIANG FUFENG BIOTECH +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a green production method of L-tryptophan by using bacterial proteolysis solution instead of yeast powder in view of the shortcomings of the existing tryptophan fermentation production technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A green production method of L-tryptophan by using bacterial protein enzymatic hydrolyzate instead of yeast powder, using bacterial cell enzymatic hydrolyzate as an organic nitrogen source to prepare a fermentation medium, and carrying out L-tryptophan fermentation. The specific steps are as follows:

[0024] Preparation of fermentation medium: glucose 8g / L, cell enzymolysis solution 300mL / L, citric acid 4.6g / L, ammonium sulfate 1.8g / L, dipotassium hydrogen phosphate 9g / L, ferrous sulfate 65mg / L, magnesium sulfate 1.6g / L, biotin 0.2 mg / L; pH controlled at 6.5-7.0, sterilized at 115°C for 15min.

[0025] The preparation method of the bacterium enzymatic solution is as follows: take the Escherichia coli bacterium in the tryptophan fermentation liquid, dilute it with water until the concentration of the dry bacterium is 18g / L, and add the same volume of 0.4mol / L of hydrochloric acid solution, mix well, treat at 100°C for 1 hour, then add trypsin to hydrolyze under its opt...

Embodiment 2

[0030] A method for producing L-tryptophan by using bacterial protein enzymatic hydrolyzate instead of yeast powder, using bacterial cell enzymatic hydrolyzate as an organic nitrogen source to prepare a fermentation medium, and carrying out L-tryptophan fermentation. The specific steps are as follows:

[0031] Preparation of fermentation medium: glucose 7.5g / L, cell enzymolysis solution 250mL / L, citric acid 4.0g / L, ammonium sulfate 1.2g / L, dipotassium hydrogen phosphate 8.5g / L, ferrous sulfate 58mg / L, Magnesium sulfate 1.6g / L, biotin 0.1 mg / L; pH controlled at 6.5-7.0, sterilized at 115°C for 15min.

[0032] The preparation method of the bacterium enzymatic solution is as follows: take the Escherichia coli bacterium in the tryptophan fermentation broth, dilute it with water until the concentration of the dry bacterium is 20g / L, and add the same volume of 0.6mol / L of hydrochloric acid solution, mix well, treat at 95°C for 1 hour, then add trypsin to hydrolyze under the optimum...

Embodiment 3

[0037] Effects of cell enzymatic hydrolyzate on cell biomass, tryptophan production and sugar-acid conversion rate.

[0038] Control group is set, control group 1: adopt the yeast powder of 10g / L to replace thalline enzymolysis solution, all the other are with embodiment 1; Control group 2: adopt the yeast powder of 5g / L to replace the thalline enzymolysis solution of half amount, All the other are the same as Example 1; Experimental group: Example 1. The specific results are shown in Table 1:

[0039] Table 1

[0040] group

[0041] Conclusion: As shown in Table 1, the technical indicators for the same period of the existing fermentation medium (control group 1) are: the highest bacterial biomass in the fermentation cycle is 43.1g / L; the yield of tryptophan is 43.2g / L, The sugar-acid conversion rate was 19.1%. Through data comparison, it can be found that the highest bacterium biomass, L-tryptophan production and sugar-acid conversion rate are all improved by usi...

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Abstract

The invention belongs to the technical field of fermentation and discloses a green production method of L-tryptophan by using thallus protease hydrolysate instead of yeast powder. The L-tryptophan canbe produced by steps as follows, separating the thallus from the fermentation broth, using trypsin for enzymatic hydrolysis after pretreatment, taking the thallus protease hydrolysate as preparationof fermentation medium by organic nitrogen sources during the fermentation process and the L-tryptophan is produced after ferment. The method improves the growth speed of the thallus and the speed ofresidual bacterial protein titer, lower the process cost sharply and has high practical value in industry.

Description

technical field [0001] The invention belongs to the field of L-tryptophan production, in particular to a green production method of L-tryptophan by using cell proteolysis solution instead of yeast powder. Background technique [0002] L-Tryptophan is one of the eight essential amino acids in human and animal life activities. It plays an important role in the growth, development and metabolism of humans and animals. It is called the second essential amino acid. In recent years, the production technology of L-tryptophan has been continuously innovated, and the production cost has been continuously reduced, making it gradually become the fourth largest amino acid additive after L-glutamic acid, L-lysine and L-methionine. [0003] The production of L-tryptophan mainly relied on protein hydrolysis and chemical synthesis at the beginning, but with the continuous deepening of research on the production of L-tryptophan by microbial methods, microbial methods have become practical an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/22C12N1/20C12R1/19
CPCC12N1/20C12P13/227
Inventor 徐庆阳李德衡王健包鑫刘元涛张宗华杨瑞丽刘子强
Owner XINJIANG FUFENG BIOTECH
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