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As(v) dual-mode detection method based on peroxidase-like properties of coooh nanosheets

A technology of peroxidase and detection method, which is applied in the measurement of color/spectral characteristics, measurement device, electrochemical variables of materials, etc., can solve the problems of expensive instruments and equipment, large demand for samples, and tedious sample pretreatment process.

Active Publication Date: 2020-12-04
NANCHANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Although the quantitative analysis of arsenic can be directly realized by atomic spectrometer, mass spectrometer or chromatographic-atomic spectrometer combined technology, these methods require expensive instruments and equipment, tedious sample pretreatment process, professional experimental technicians, and instruments. The maintenance cost is high and the demand for samples is large. However, the spectral analysis method based on functionalized nanomaterials is suitable for the analysis and detection of almost all common pollutant ions due to the simple equipment used, and is more suitable for the quantitative analysis of arsenic.

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  • As(v) dual-mode detection method based on peroxidase-like properties of coooh nanosheets
  • As(v) dual-mode detection method based on peroxidase-like properties of coooh nanosheets
  • As(v) dual-mode detection method based on peroxidase-like properties of coooh nanosheets

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Experimental program
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Effect test

Embodiment 1

[0032] Preparation and Characterization of CoOOH Nanosheets

[0033] Preparation of CoOOH nanosheets: Mix 1 mL of 1.0M NaOH solution with 4 mL of 10 mM CoCl 2 Mix the solution, stir vigorously for 1 min, then add 200 μL of 0.9M NaClO solution, mix thoroughly, and sonicate for 20 min in an ultrasonic instrument with a power of 500 W to obtain a nearly black solution, centrifuge at a centrifugal speed of 12000 rpm and use ultrapure water repeatedly Wash until the pH of the obtained solution is neutral, disperse the obtained CoOOH nanosheets in 1 mL of ultrapure water to make a CoOOH nanosheet solution with a concentration of 50 μg / mL, and store at 4 °C;

[0034] Characterization of CoOOH nanosheets:

[0035] For CoOOH and CoCl 2 The solution was characterized by UV-Vis absorption spectroscopy, and the results were as follows: figure 1 shown. Depend on figure 1 Visible, CoCl 2 The solution has a characteristic absorption peak at 512nm, while the CoOOH nanosheets prepared by...

Embodiment 2

[0040] Analysis of the peroxidase activity of CoOOH nanosheets: The effect of CoOOH nanosheets on H 2 o 2 -Peroxidase-like activity of the ABTS system, results such as image 3 As shown in A. Depend on image 3 A is visible, CoOOH, actatebuffer, H 2 o 2 , ABTS, CoOOH+ABTS, actate buffer+ABTS and H 2 o 2 +actate buffer has no obvious characteristic absorption peak change and color change (curve a-g), but CoOOH+actate buffer+ABTS+H 2 o 2 The solution is obviously green, and ABTS appears at 418nm, 650nm and 740nm ox The three characteristic absorption peaks (curve h), the above results show that CoOOH nanosheets have good peroxidase-like activity and can effectively catalyze H 2 o 2 - ABTS in the ABTS system is oxidized to produce green ABTS ox .

[0041] In addition, the inhibitory effect of As(V) on the peroxidase activity of CoOOH nanosheets was investigated by UV-visible absorption spectroscopy. The results are as follows: image 3 Shown in B. Depend on image ...

Embodiment 3

[0045] Detection of As(V) by Absorption Spectrometry Based on Peroxidase-like Properties of CoOOH Nanosheets

[0046] In order to achieve the best analysis of As(V), the experimental conditions affecting the color development of the substrate were optimized. The enzyme-catalyzed reaction between CoOOH nanosheets and ABTS was selected at pH 4.0, the concentration of ABTS was 0.05 mM, and the H 2 o 2 The concentration of ABTS is 1.8mM, the reaction time of ABTS is 30min, the pH of CoOOH and As(V) reaction is 7.5 and the reaction time is 120min.

[0047] Under optimized conditions, the analytical performance of CoOOH nanosheets as a colorimetric probe for As(V) was investigated. The results are as follows: Figure 5 shown. Add 5 μL of 2.8 mg / mL CoOOH solution to 100 μL of 100 mM pH 7.5 HEPES buffer solution and mix well, then add different concentrations of As(V) solutions, dilute the total volume of the reaction solution with ultrapure water to 200 μL, shake The bed was shak...

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Abstract

The invention discloses an As(V) dual-mode detection method based on a peroxidase-like characteristic of a CoOOH nanosheet, and belongs to the field of absorption spectrums and electrochemical sensing. The method comprises the following steps: firstly, the CoOOH nanosheet is synthesized, and when H2O2 exists, CoOOH can catalyze a developing substrate AABTS to be converted into a green oxidation product ABTSox; when As(V) exists, As(V) is combined onto the surface of the CoOOH under the electrostatic action and the action of an As-O bond, so that the catalysis activity of the CoOOH to the ABTSis inhibited, leading to reduction of the green oxidation product ABTSox and color lighting, and detection of the As(V) can be realized according to an absorption spectrum; in addition, the CoOOH is modified onto the surface of a glassy carbon electrode, and when the As(V) is adsorbed onto the surface of the CoOOH modified electrode, high-sensitivity electrochemical analysis of the As(V) can be realized according to an attenuation degree of reducing current. The dual-mode detection method disclosed by the invention has the advantages of simplicity, sensitivity, visibility, dual-mode detectionand the like.

Description

technical field [0001] The invention discloses an As(V) dual-mode detection method based on the properties of CoOOH nanosheet-like peroxidase, which belongs to the field of absorption spectrum and electrochemical sensing. Background technique [0002] The pollution of human drinking water and food by arsenic in water and soil will directly endanger the sound development of ecology and the healthy existence of human beings. Pentavalent arsenic is toxic to plants mainly because pentavalent arsenic forms unstable (ADP)-As complexes with adenosine diphosphate and disrupts energy flow in cells. Since arsenate (As(V)) and phosphate (PO 4 3- ) structure and chemical properties are highly similar, As(V) can participate in physiological processes and other activities by entering bacteria or plant root cells to replace phosphoric acid. Arsenic is mainly in the form of inorganic arsenic (As(III) and As(V)) in water or soil, and in the form of As(V) under oxidizing conditions. The f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/33G01N27/30
CPCG01N21/33G01N27/308
Inventor 邱建丁问少华梁汝萍
Owner NANCHANG UNIV
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