Core-shell nano drug for tumor in-situ visual therapy and preparation method thereof

A nano-drug, core-shell technology, applied in nano-drugs, anti-tumor drugs, preparations for in vivo tests, etc., can solve the problems of delayed treatment timing, low diagnosis and treatment efficiency, and long interval time.

Active Publication Date: 2018-10-09
UNIV OF ELECTRONICS SCI & TECH OF CHINA
View PDF4 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the diagnosis and treatment of tumors are usually two relatively independent processes in traditional clinical applications, diagnostic reagents...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Core-shell nano drug for tumor in-situ visual therapy and preparation method thereof
  • Core-shell nano drug for tumor in-situ visual therapy and preparation method thereof
  • Core-shell nano drug for tumor in-situ visual therapy and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] A core-shell nanomedicine for in situ visualization therapy of tumors, such as figure 1 As shown, it is characterized in that: a core-shell nano-medicine for in situ visualized treatment of tumors, characterized in that: the core of the core-shell nano-medicine includes: polycationic compound, and the polycationic compound passes through The immune adjuvant oligonucleotides combined by electrostatic attraction, the immune adjuvant oligonucleotides are labeled with fluorescent probes; the shell of the core-shell nano-medicine includes: a hydrophilic shell, and a hydrophilic shell An MRI imaging contrast agent chelated on the surface of the shell, the hydrophilic shell includes a negatively charged phototherapy drug modified by polydopamine, and the phototherapy drug is formed by copolymerization of aminated graphene quantum dots and a carboxyl-containing photosensitizer ; The core-shell structure is formed by electrostatic assembly between polycationic compounds and poly...

Embodiment 2

[0088] Stability experiment of nanomedicine:

[0089] Such as Figure 5 As shown, figure (a) is the different incubation time of nano-medicine of the present invention and DNase I enzyme, and figure (b) is the protective effect of different nanoparticles on CpG, wherein M is Maker, 1 is free CpG, and 2 is CpG+DNase I Enzyme, 3 is P / C+DNase I enzyme+heparin sodium, 4 is P / C+heparin sodium+DNase I enzyme, 5 is P / C@GCpD(Gd)+DNase I enzyme+heparin sodium, 6 is P / C @GCpD(Gd) + sodium heparin + DNase I enzyme.

[0090] Through agarose gel electrophoresis experiments, it was shown that the P / C@GCpD(Gd) nanoparticles of the present invention can protect CpG from being degraded by enzymes. Compared with free CpG, the P / C@GCpD(Gd) particles of the present invention can Improve the circulation time of CpG in the body to a certain extent.

[0091] Such as Figure 6 As shown, figure (a) and figure (b) are respectively the change figure of particle size and PDI of P / C@GCpD (Gd) of the p...

Embodiment 3

[0093] In vitro photothermal and photodynamic properties experiments of nanomedicine:

[0094] Such as Figure 7 As shown, under 660nm laser excitation (1W / cm 2 , 10 minutes), the nanomedicine P / C@GCpD(Gd) of the present invention has a good photothermal heating effect measured by an infrared thermal imager and a thermocouple; P / C@GCpD(Gd) was measured by a singlet oxygen probe SOSG ( Gd) has a high singlet oxygen yield and can be used in photodynamic experiments.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Surface potentialaaaaaaaaaa
Particle sizeaaaaaaaaaa
Login to view more

Abstract

The invention discloses core-shell nano drug for tumor in-situ visual therapy and belongs to the technical field of nano medicine. The preparation method includes: adopting a nanoparticle formed by assembling CpG-OND marked with a fluorescent probe and a polycationic compound as a core, self-assembling graphene quantum dots with the surfaces modified by polydopamine and a photosensitizer copolymerization product on the surface of the core as a shell, and further chelating a MRI ion contrast agent on the shell to form the core-shell nano drug. The nano drug has excellent photodynamic/photothermal anticancer effect and good immunostimulation effect; lesion imaging can be realized through a MRI imaging contrast agent and the fluorescent probe, the nano drug has visual characteristics, noninvasive and real-time tracking of distribution, absorption and metabolism of the nano drug is realized, and the nano drug can be used for prognosis detection further. The nano drug integrates combined treatment and multimode imaging diagnosis functions, realizes tumor diagnosis and treatment integration and has wide application prospect in the field of tumor diagnosis and treatment.

Description

technical field [0001] The invention belongs to the technical field of nano-medicine, and in particular relates to a core-shell nano-medicine for in-situ visualized treatment of tumors and a preparation method thereof. Background technique [0002] Due to its high degree of heterogeneity, complex microenvironment and other factors, malignant tumor is a major disease that seriously endangers public life and health, and has become a global public health problem. At present, single treatment methods, such as surgical resection, chemotherapy, radiotherapy, etc., have poor efficacy and large side effects; combining or synergistically killing tumors with multiple treatment methods is the mainstream strategy of current clinical treatment. At the same time, emerging tumor therapies, such as tumor phototherapy and immunotherapy, have emerged one after another. The development and combined use of tumor therapies provide new hope for tumor treatment. Tumor phototherapy uses laser to e...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K49/12A61K49/18A61K9/51A61K39/39A61K41/00A61K47/55A61K47/59A61K49/00A61P35/00B82Y5/00
CPCA61K9/5146A61K39/39A61K41/0052A61K41/0057A61K49/0002A61K49/0052A61K49/0093A61K49/126A61K49/1824A61K2039/6025A61K47/55A61K47/59A61P35/00B82Y5/00A61K2300/00
Inventor 吴春惠官晓天田原刘秋月刘贻尧杨红李顺秦翔
Owner UNIV OF ELECTRONICS SCI & TECH OF CHINA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap