Tripterygium wilfordii triterpene synthase twosc1 and its coding gene and application

A coding and amino acid technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problems of limited development, low content, slow plant growth, etc.
CN108866030BActive Publication Date: 2019-05-07CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CAPITAL UNIVERSITY OF MEDICAL SCIENCES
Publication Date
2019-05-07

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Abstract

A Tripterygium wilfordii triterpene synthase TwOSC1 protein and a coding gene thereof. cDNA of a TwOSC1 gene is cloned to eukaryotic expression vector pYES2, and a recombinant expression vector carrying the TwOSC1 gene is constructed and transformed into a yeast expression host, allowing triterpenoids such as friedelin and amyrins to be produced. Mutation research has shown that amino acids 486 and 502 in the code of the TwOSC1 gene are key sites able to increase or decrease production of friedelin or amyrins. Gene gun-mediated interference experimentation has shown that interference of the TwOSC1 gene has a marked inhibiting effect on the synthesis of celastrol in Tripterygium wilfordii, and further gene gun-mediated overexpression experimentation has shown that overexpression of the TwOSC1 gene can increase production of celastrol in Tripterygium wilfordii suspension cells. TwOSC1 protein and the coding gene therefor may be used in biosynthesis of plant triterpenoids, and in cultivating high-quality Tripterygium wilfordii.
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Description

technical field

[0001] The invention relates to tripterygium wilfordii triterpene synthase and its encoding gene, and the application of the triterpene synthase and its encoding gene in the biosynthesis of triterpenoids, belonging to the field of medicinal plant genetic engineering. Background technique

[0002] The traditional Chinese medicine Tripterygium wilfordii, derived from the xylem of the dried root of the medicinal plant Tripterygium wilfordii. Hook.f., is widely used in the treatment of rheumatoid arthritis and inflammation (Raphaela G M, Mildred W, Roy F, et al .Comparison of Tripterygium wilfordii Hook F Versus Sulfasalazine in the Treatment of Rheumatoid Arthritis:A Randomized Trial[J].Annals of Internal Medicine,2009,151(4):229-240.Tao X L,Lipsky P E.The Chinese anti-inflammatory and immunosuppressive herbal remedy Tripterygium wilfordii Hook F. [J]. Rheumatic Disease Clinics of North America, 2000, 26(1):29–50.). Terpenes are the main active ingredients of T...

Claims

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