Unknown gene cloning method and application thereof
A gene and purpose technology, applied in the field of cloning unknown genes, can solve the problems of restricting the large-scale popularization and use of probiotics, high production and application costs, and low proportion of active ingredients, so as to reduce the occurrence of breeding diseases, improve the survival rate, and improve the Yield effect
Inactive Publication Date: 2018-11-23
HUAIHAI INST OF TECH
View PDF2 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
[0003] At present, many probiotics are being demonstrated and promoted in production, but the outstanding problems are that the product performance is unstable, the proportion of active ingredients is low, and the cost of production and application is high, which seriously limits the application of probiotics. Widespread use
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0043] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Apparently, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.
[0044] The invention provides a technical solution: a method for cloning an unknown gene, comprising the following steps:
[0045] S1: Protein sequence determination and data analysis;
[0046] S2: Sampling the protein bands obtained by the above protein electrophoresis, and putting the sampled protein bands into the detection equipment for secondary mass spectrometry analysis;
[0047] S3: Summary of predicted protein sequence data:
[0048] ①According to the protein infor...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention discloses an unknown gene cloning method and application thereof, wherein the unknown gene cloning method comprises the following steps: S1: A protein sequencing and data analysis are made; S2: Protein bands obtained through performing electrophoresis on the above proteins are sampled and put in testing equipment for secondary mass spectrographic analysis; S3: Predicted protein sequencing data are summarized and sorted out. The unknown gene cloning method and the application thereof can guarantee stable product performance, enhance efficiency, have high stability, reduce production and application cost, and boost massive promotion and use of probiotics, by ascertaining genes that control active ingredients for antagonism in antagonistic bacteria and cloning such genes in a molecular biology means, an engineered is obtained strain and then mass production of the strain is achieved through a fermentation engineering technology and high-throughput expression is performed onthe genes to ultimately provide stable, high-yield and efficient microbial preparations for production.
Description
technical field [0001] The invention relates to the technical field of cloning unknown genes, in particular to a method for cloning unknown genes and its application. Background technique [0002] The upstream engineering of genetic engineering is mainly the preparation and asexual reproduction of the target gene. Specifically, the target gene is prepared from the tissues, organs or cells of the organism or the target gene is artificially synthesized, the target gene is spliced with the DNA of the carrier, the recombinant molecule is introduced into the recipient cell, and screening and asexual reproduction are performed. This process can be called gene cloning. Gene cloning is a revolutionary research technology developed in the 1970s, which can be summarized as: dividing, cutting, connecting, transferring, and selecting. The ultimate goal is to introduce the target gene into the host cell through corresponding technical means, and the target gene is copied in large qua...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10C12N15/70G01N33/68G06F19/18
CPCC12N15/10C12N15/70G01N33/6818G01N33/6848C12Q2531/113
Inventor 李联泰安贤惠杨乔乔顾金付陆红艳
Owner HUAIHAI INST OF TECH