Post-treatment method and post-treatment device for polyacrylamide gel electrophoresis products

A polyacrylamide gel and post-processing device technology, applied in the biological field, can solve the problems of unobservable bands, high toxicity of fluorescent dye EB, and large amount of sample loading, so as to achieve clear gel scanning results and excellent post-processing The effect is good and the effect of improving the experimental efficiency

Pending Publication Date: 2018-11-23
河北省农林科学院粮油作物研究所
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

Compared with polyacrylamide gel electrophoresis, agarose gel electrophoresis is fast and simple, but the number of samples to be tested at one time is small, the required sample volume is large, the resolution is low, and the fluorescent dye EB is highly toxic; polyacrylamide gel electrophoresis detection can For the detection of large batches of samples, the required sample amount is small, the resolution is high, and the bands with a difference of several base pairs can be detected, and the operator is far awa

Method used

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  • Post-treatment method and post-treatment device for polyacrylamide gel electrophoresis products
  • Post-treatment method and post-treatment device for polyacrylamide gel electrophoresis products
  • Post-treatment method and post-treatment device for polyacrylamide gel electrophoresis products

Examples

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Example Embodiment

[0057] Example 1

[0058] Provide turnover board and experiment box, the turnover board is PP board, length 33.0cm, width 21.2cm, thickness 0.1cm, leave 0.95cm on both sides of the long side, the diameter of the holes is 0.7cm, the hole spacing is 0.82cm, total punching 21; leave 1.13cm on both sides of the wide side, the hole diameter is 0.7cm, the hole spacing is 0.82cm, a total of 13 holes are punched; the vertical distances between the four corners and the wide side and the long side are 0.5cm, respectively Make four small holes with a diameter of 0.4cm, and then tie a rope with a length of 29.0cm on both sides; the actual photo of the turnover board is as follows figure 1 Shown.

[0059] The experiment box includes a washing box, a dyeing box and a developing box. The dimensions are all: inner diameter length 34.0cm, inner diameter width 22.0cm, height 9.5cm, and box thickness 2.0cm. The actual photo of the experiment box is as figure 2 Shown.

[0060] Use polyacrylamide gel ...

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Abstract

The invention provides a post-treatment method and a post-treatment device for polyacrylamide gel electrophoresis products. The post-treatment device comprises a turnover plate and experiment boxes, and the experiment boxes include washing boxes, a dyeing box and a color developing box; the washing boxes include a first washing box, a second washing box and a third washing box. The post-treatmentmethod and the post-treatment device have the advantages that gel is placed on the turnover plate during application, and washing, dyeing and color development of the gel are achieved by moving the turnover plate in the different experimental boxes; the turnover plate is moved only by lifting a handle, so that hands are prevented from directly contacting with the gel and reagents; the gel is not easily broken during experiments, drug contamination is reduced, and the experimental efficiency is improved; when the polyacrylamide gel electrophoresis products are post-treated by the device, the steps are simple, the post-treatment effect is good, and the treated gel has clear picture scanning results.

Description

technical field [0001] The invention relates to the technical field of biology, in particular to a post-processing method and device for gel electrophoresis products. Background technique [0002] Currently, PCR amplification products are generally detected using agarose gel electrophoresis or polyacrylamide gel electrophoresis detection systems. Compared with polyacrylamide gel electrophoresis, agarose gel electrophoresis is fast and simple, but the number of samples tested at one time is small, the required sample volume is large, the resolution is low, and the fluorescent dye EB is highly toxic; polyacrylamide gel electrophoresis detection can For the detection of a large number of samples, the required sample amount is small, the resolution is high, and the bands with a difference of several base pairs can be detected, and the operator is far away from the contamination of EB. [0003] However, in the traditional polyacrylamide gel electrophoresis, when washing, stainin...

Claims

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Application Information

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IPC IPC(8): G01N1/31
CPCG01N1/31
Inventor 胡亚亚马志民焦伟静高志远韩美坤刘兰服
Owner 河北省农林科学院粮油作物研究所
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