Post-treatment method and post-treatment device for polyacrylamide gel electrophoresis products

Abstract

The invention provides a post-treatment method and a post-treatment device for polyacrylamide gel electrophoresis products. The post-treatment device comprises a turnover plate and experiment boxes, and the experiment boxes include washing boxes, a dyeing box and a color developing box; the washing boxes include a first washing box, a second washing box and a third washing box. The post-treatmentmethod and the post-treatment device have the advantages that gel is placed on the turnover plate during application, and washing, dyeing and color development of the gel are achieved by moving the turnover plate in the different experimental boxes; the turnover plate is moved only by lifting a handle, so that hands are prevented from directly contacting with the gel and reagents; the gel is not easily broken during experiments, drug contamination is reduced, and the experimental efficiency is improved; when the polyacrylamide gel electrophoresis products are post-treated by the device, the steps are simple, the post-treatment effect is good, and the treated gel has clear picture scanning results.

Description

technical field [0001] The invention relates to the technical field of biology, in particular to a post-processing method and device for gel electrophoresis products. Background technique [0002] Currently, PCR amplification products are generally detected using agarose gel electrophoresis or polyacrylamide gel electrophoresis detection systems. Compared with polyacrylamide gel electrophoresis, agarose gel electrophoresis is fast and simple, but the number of samples tested at one time is small, the required sample volume is large, the resolution is low, and the fluorescent dye EB is highly toxic; polyacrylamide gel electrophoresis detection can For the detection of a large number of samples, the required sample amount is small, the resolution is high, and the bands with a difference of several base pairs can be detected, and the operator is far away from the contamination of EB. [0003] However, in the traditional polyacrylamide gel electrophoresis, when washing, stainin...

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Problems solved by technology

Compared with polyacrylamide gel electrophoresis, agarose gel electrophoresis is fast and simple, but the number of samples to be tested at one time is small, the required sample volume is large, the resolution is low, and the fluorescent dye EB is highly toxic; polyacrylamide gel electrophoresis detection can For the detection of large batches of samples, the required sample amount is small, the resolution is high, and the bands with a difference of several base pairs can be detected, and the operator is far away from the contamination of EB

[0003] However, in the traditional polyacrylamide gel electrophoresis, when washing, staining and developing, the experimenter will put the gel in different reagents. Although the gloves are worn, there are leaks in the gloves, and the skin is directly exposed to silver nitrate or formaldehyde, etc. The risk of reagents, and when the gel is switched between different reagents, it is easy to break, so that the complete band cannot be observed, which affects the electrophoresis results

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