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Graphene QuEChERS method-based UPLC-MS/MS detection method established for liposoluble phycotoxins

A detection method, shellfish toxin technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problem of single detection type, achieve the effects of improving sensitivity, less contact with organic solvents, and reducing matrix benefits

Active Publication Date: 2018-11-23
ZHOUSHAN INST FOR FOOD & DRUG CONTROL
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Problems solved by technology

[0004] The purpose of the present invention is to solve the existing mass spectrometry detection method for shellfish toxins, and to provide a method for reducing the matrix benefit and improving the sensitivity of the method to establish fat-soluble shellfish toxins using the QuEChERS method based on graphene oxide. UPLC-MS / MS multi-component quantitative detection method

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  • Graphene QuEChERS method-based UPLC-MS/MS detection method established for liposoluble phycotoxins
  • Graphene QuEChERS method-based UPLC-MS/MS detection method established for liposoluble phycotoxins
  • Graphene QuEChERS method-based UPLC-MS/MS detection method established for liposoluble phycotoxins

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Embodiment Construction

[0021] Below in conjunction with specific embodiment and accompanying drawing, the present invention will be further explained:

[0022] laboratory apparatus

[0023] Waters ultra-high liquid phase system (ACQUITYUPLC) and triple quadrupole tandem mass spectrometer (XEVO-TQ), including electrospray ionization source (ESI) and atmospheric pressure chemical ionization source (APCI) (Waters, USA);

[0024] Chromatographic column: Waters XBridge C18 column (5μm, 100mmX2.1mm i.d.) Acquity HSS-T 3 column(1.8μm,, 100mm×2.1mm i.d.,) Atlantics HILIC Silica column(Waters, 150mm×2.1mm i.d., 5μm) (Waters, MA, USA);

[0025] Pure water instrument: ELGA PURELAB Ultra+HitechRoDI plus pure water preparation instrument (ELGA, UK);

[0026] Homogenizer: PoMron (PT2000, Switzerland);

[0027] Refrigerated centrifuge: Type 3K-18 (Sigma, Germany);

[0028] Vortex oscillator: SCILOGXE MX-S (SCILOGEX, USA);

[0029] Tissue masher: IKA T-18 (IKA, Germany);

[0030]Analytical balance: ELECTRONIC...

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Abstract

The invention relates to a graphene QuEChERS method-based UPLC-MS / MS detection method established for liposoluble phycotoxins. The UPLC-MS / MS quantitative detection method for the nine liposoluble phycotoxins in shellfish aquatic products is established. The method has the advantages of rapidness, simplicity, convenience, high efficiency, high recycle rate, less contact with organic solvent, timesaving and the like. By the established QuEChERS sample pre-treatment technology, the freezing and degreasing technology and the high adsorption property of graphene oxide, complex matrixes (such as free fatty acid and pigment) can be removed effectively, the good detection recovery effect on the liposoluble phycotoxins can be obtained, and the purification effect is good. The low-temperature refrigeration technology is introduced to assist the QuEChERS method in purifying samples, so that matrix benefits can be reduced remarkably, and the sensitivity of the methods can be improved. The UPLC-MS / MS technology is combined, detection of the liposoluble phycotoxins in shellfish samples is applied successfully, the nine liposoluble phycotoxins can be detected at the same time, more five liposoluble phycotoxins are detected than existing standard methods, and high applicability is achieved.

Description

technical field [0001] The invention relates to a detection method for fat-soluble shellfish toxins, in particular to a UPLC-MS / MS multi-component quantitative detection method for fat-soluble shellfish toxins established by the QuEChERS method based on graphene oxide, which reduces the matrix benefit and improves the sensitivity of the method . Background technique [0002] Marine biotoxins (shellfish toxins) specifically refer to a large class of small molecule toxic chemicals that are mainly produced by marine toxic microalgae or microorganisms, can be enriched in marine organisms, especially bivalve molluscs, and are harmful to other organisms including humans. substance. For these biological toxins, researchers initially divided them into six categories based on the symptoms of poisoning they caused: Paralytic Shellfish Poisoning (PSP), Diarrhetic Shellfish Poisoning (DSP), Neurotoxicity Shellfish poisoning (Neurotoxic Shellfish Poisoning, NSP), amnesic shellfish pois...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/08
CPCG01N30/02G01N30/08
Inventor 赵巧灵王萍亚黄朱梁戴意飞陈翔蒋玲波
Owner ZHOUSHAN INST FOR FOOD & DRUG CONTROL
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