Measuring method of phenol content in water

Abstract

The invention discloses a measuring method of a phenol content in water. According to a key point of the technical scheme, the measuring method comprises the following steps: step 1: placing 10 to 50mL of to-be-detected water sample into a pressure container, adding 5 to 20 g of sodium chloride, and adding 5 to 20 mL of organic solvent; step 2: heating the container in a muffle furnace, naturallycooling to the room temperature, acquiring an upper layer organic solvent, and concentrating by utilizing a rotary evaporator; step 3: purifying by virtue of a solid-phase extraction column, and collecting effluent and eluent; step 4: concentrating until almost dry by utilizing a nitrogen blower, adding a solvent for compositely dissolution, and filtering by utilizing 0.02 to 0.45 micro meter oforganic filter membrane; and step 5: testing phenol by using a high-performance liquid-phase chromatographic instrument, wherein a liquid-phase chromatographic column of C8 or C18 is selected as the chromatographic column; and analyzing by utilizing a fluorescent detector. By utilizing the measuring method of the phenol content in water, the phenol content in the sample can be simply, rapidly, accurately, qualitatively and quantitatively detected.

Description

【Technical field】 [0001] The invention belongs to the technical field of food detection, and in particular relates to a method for measuring phenol content in water. 【Background technique】 [0002] People's requirements for food safety are getting higher and higher, and the limits of harmful substances in drinking water, surface water and agricultural irrigation water are becoming stricter and stricter. Phenol is widely used as a raw material for plastics, as an important raw material for producing resins, fungicides, preservatives and pharmaceuticals. However, phenol is a toxic substance, which has a strong corrosive effect on the skin and mucous membranes, can inhibit the central nervous system or damage liver and kidney functions; inhalation of high-concentration steam can cause headache, dizziness, fatigue, blurred vision, pulmonary edema, etc.; Taking it can cause digestive tract burns, burning pain, exhaled breath with phenol smell, vomit or stool with blood, there is...

AI Technical Summary

Problems solved by technology

However, the above method has the following defects: the pretreatment method needs to distill the water sample, then collect the distillate, and develop the color of the distillate, or use an organic solvent after the color development to extract and concentrate the color-developed material for testing. The process is complex and cumbersome, the pH value of the water needs to be considered, and a wide variety of reagents need to be configured, and the process is difficult to control. When using colorimetric testing, the color of the reagents and the color of the water sample will seriously interfere with the test results

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