Method for measuring nitrogen cycling rate in sediment based on continuous process

A measurement method and sediment technology, which is applied in the field of sediment nitrogen cycle rate measurement based on continuous processes, can solve the problems of overestimating the overall sediment nitrogen cycle rate and failing to reflect the continuity of different processes, and achieve the effect of simple operation

Inactive Publication Date: 2018-11-23
INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI +1
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The traditional method measures step by step, which may overestimate the overall sedimen

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  • Method for measuring nitrogen cycling rate in sediment based on continuous process

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[0014] Below in conjunction with accompanying drawing and embodiment the method of the present invention will be further described:

[0015] The specific implementation method of the sediment nitrogen cycle determination method based on the continuous process of the present invention is as follows figure 1 shown, including the following steps:

[0016] Step 1: Weigh 10 equal portions of fresh sediment samples after mixing (approximately equivalent to 10g dry weight, mix well in the sample bag, it is not suitable to open the bag mouth to mix) into ten 100mL serum bottles, use silica gel stoppers and aluminum cover seal;

[0017] Step 2: Immediately evacuate the sealed serum bottle and wash it with helium 3 times to remove N 2 , then inject 50ml O 2 After equilibrating for 5 minutes, utilize a syringe to release the overpressure gas in the headspace of the serum bottle, and the helium in the headspace gas: the final ratio of oxygen is 2:1;

[0018] Step 3: Inject 10 mL of ur...

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Abstract

The invention discloses a method for measuring nitrogen cycling rate in sediment based on a continuous process, which is applied to the measurement of nitrogen cycling process in the field of ecological environment. The specific process is that: 10 equal parts of fresh sediments are weighed and taken into corresponding 100 mL serum bottles; after the serum bottles are capped and sealed, air in theheadspace of the serum bottles is replaced as mixed gas of helium and oxygen with a ratio of 2:1 by a vacuum bottle washing system; urea and deionized water are added separately; incubation is performed at 25 DEG C for 120 hours; then the gaseous nitrogen content in the headspace and the active nitrogen content in the sediment are measured separately; according to the content of various forms ofnitrogen in a treatment group and a control group, activity of the sediments in denitrification, nitrification and ammoniation processes is calculated by the use of corresponding formulas. The methodhas the advantages that operation is simple, the time cost of the traditional step-by-step measurement method is reduced; meanwhile, based on the continuous process, the reaction rate in each processof the sediment nitrogen cycle is accurately measured, and the synergistic relationship of three continuous processes is reflected, thereby avoiding the overestimation of sediment nitrogen cycling rate resulting from substrate induction measuring method.

Description

technical field [0001] The invention belongs to the field of ecological environment research, and in particular relates to a method for measuring sediment nitrogen cycle rate based on a continuous process. Background technique [0002] The nitrogen cycle is an ecosystem process closely related to water environment quality, atmospheric chemistry, and primary production. It is an important ecosystem function and is related to ecosystem production services. The measurement of nitrogen cycle and related microbial activity is widely used to characterize the function of various ecosystems and the adaptation of ecosystems to external disturbances. At present, the traditional soil and sediment nitrogen cycle rate measurement is completed by the step-by-step substrate induction method, that is, urea (or amino acid), ammoniacal nitrogen and nitrate are used as substrates, and the yield of the final product of the reaction is measured after a certain period of incubation. (Amination...

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Application Information

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IPC IPC(8): G01N33/24
CPCG01N33/24
Inventor 俞慎丁晶
Owner INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI
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