Two-step method for preparing high-purity medium-chain and long-chain triglyceride
A triglyceride, medium and long chain technology, applied in the field of biopharmaceutical emulsifier preparation, can solve problems such as product purity reduction and acyl transfer, and achieve the effects of increasing content, ensuring activity, and improving yield
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[0029] A method for preparing high-purity medium and long-chain triglycerides, the method and other process routes such as figure 1 shown, including the following steps:
[0030] (1) Place 8 g of immobilized enzyme Lipozyme435 on the carrying mechanism of the bubbling reactor, start the water bath mechanism to heat the reactor body to 70°C;
[0031] (2) Add 51g caprylic acid triglyceride (containing about 0.1mol medium-chain triglyceride) and 100g soybean oil (combining 0.1mol long-chain triglyceride) in the feed tank as reactants, and the reaction in the feed tank The material is preheated to 70°C, and then put into a bubbling reactor for transesterification reaction. Nitrogen is introduced into the reaction material and the nitrogen flow rate is controlled to be 2cm / s, so that the nitrogen gas is continuously blown into the main body of the reactor through the sieve plate, so that the material Mix well and carry out the transesterification reaction at a constant temperature...
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