Ganoderma lucidum feed additive for litopenaeus vannamei cultured in low-salt environment and application of ganoderma lucidum feed additive
A feed additive, the technology of Vannabin, applied in the field of aquaculture feed, can solve the problems that there are no relevant research reports on Ganoderma lucidum polysaccharides and its amino acid extracts, unfavorable cultivation of large-scale individuals, low survival rate, and low growth status, and achieve Improve the survival rate, good survival rate, and improve the effect of growth
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[0030] A low-salt cultured Litopenaeus vannamei Ganoderma lucidum feed additive includes Ganoderma lucidum polysaccharides and Ganoderma lucidum amino acids. The mass percentages of Ganoderma lucidum polysaccharides and Ganoderma lucidum amino acids are shown in Table 1.
[0031] The preparation method of Ganoderma lucidum polysaccharide is:
[0032] The Ganoderma lucidum fruit body was crushed into powder, added with 25 times the amount of water, and extracted at 80°C for 2.5 hours to obtain the extract. Add ethanol and alcohol precipitation to a concentration of 80%, wash with ethanol and acetone 3 times, then Add Sevag reagent to deproteinize, dry, add to 80% ethanol for precipitation to obtain Ganoderma lucidum polysaccharide.
[0033] The preparation method of Ganoderma lucidum amino acid is:
[0034] Ganoderma lucidum fruit body was crushed into powder, 15 times the amount of 70% ethanol was added, and extracted for 3 hours to obtain the crude maca amino acid extract. The dilute...
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