Preparation and amplification method for T cells with terminal malignant tumor lung metastasis with malignant pleural effusion and application
A technology for malignant pleural effusion and malignant tumors, which is applied in the field of preparation and expansion of T cells with malignant tumor lung metastasis and malignant pleural effusion, achieving convenient technology, high cytotoxicity, high tumor targeting and killing ability Effect
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Embodiment 1
[0065] The acquisition of embodiment 1 PEMC, expansion, the acquisition of primary tumor cell
[0066] 1. Acquisition of PEMC
[0067] The obtaining of PEMC refers to separating from interstitial fluid after being treated and cultured in malignant pleural effusion of patients with lung metastases.
[0068] (1) The pleural cavity drainage fluid collected clinically removes impurities in the pleural effusion through a 40 μm cell strainer under aseptic conditions. The index of the present invention is the closed pleural cavity drainage institute for patients with bone and soft tissue malignant tumor lung metastasis and malignant pleural effusion. The tissue fluid collected from drainage;
[0069] (2) According to the requirements of step (1), the drained pleural tissue fluid was aseptically divided into 50ml centrifuge tubes, and the supernatant was removed after centrifugation at 300G for 5 minutes, and the bloody liquid deposition in the lower layer was collected; the collecte...
Embodiment 2
[0100] The effect evaluation of embodiment 2PEMC
[0101] 1 information
[0102] 1.1 General information
[0103] 3 clinical patients. The first patient was a 19-year-old male with pulmonary metastasis of left femoral osteosarcoma and left pleural effusion (Enneking stage III); the second patient was a 23-year-old female with pulmonary metastasis of right humeral osteosarcoma and right pleural effusion (Enneking stage III); the third patient was a 25-year-old male diagnosed with pulmonary metastasis of pelvic osteosarcoma with right pleural effusion (Enneking stage III).
[0104] 1.2 Diagnostic criteria
[0105] Chest X-ray often presents as a solitary spherical lesion, generally less than 2 cm, mostly round, with smooth edges and light density, without obvious burrs and lobulation features.
[0106] 1.3 Inclusion criteria
[0107] (1) Meet the above diagnostic criteria for lung metastatic tumors;
[0108] (2) Between the ages of 18 and 60, male or female;
[0109] (3) ...
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