Preparation and amplification method for T cells with terminal malignant tumor lung metastasis with malignant pleural effusion and application

A technology for malignant pleural effusion and malignant tumors, which is applied in the field of preparation and expansion of T cells with malignant tumor lung metastasis and malignant pleural effusion, achieving convenient technology, high cytotoxicity, high tumor targeting and killing ability Effect

Inactive Publication Date: 2018-12-04
SHANGHAI FIRST PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report about the T cell preparation and expansion method and application of...

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  • Preparation and amplification method for T cells with terminal malignant tumor lung metastasis with malignant pleural effusion and application
  • Preparation and amplification method for T cells with terminal malignant tumor lung metastasis with malignant pleural effusion and application
  • Preparation and amplification method for T cells with terminal malignant tumor lung metastasis with malignant pleural effusion and application

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Experimental program
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Embodiment 1

[0065] The acquisition of embodiment 1 PEMC, expansion, the acquisition of primary tumor cell

[0066] 1. Acquisition of PEMC

[0067] The obtaining of PEMC refers to separating from interstitial fluid after being treated and cultured in malignant pleural effusion of patients with lung metastases.

[0068] (1) The pleural cavity drainage fluid collected clinically removes impurities in the pleural effusion through a 40 μm cell strainer under aseptic conditions. The index of the present invention is the closed pleural cavity drainage institute for patients with bone and soft tissue malignant tumor lung metastasis and malignant pleural effusion. The tissue fluid collected from drainage;

[0069] (2) According to the requirements of step (1), the drained pleural tissue fluid was aseptically divided into 50ml centrifuge tubes, and the supernatant was removed after centrifugation at 300G for 5 minutes, and the bloody liquid deposition in the lower layer was collected; the collecte...

Embodiment 2

[0100] The effect evaluation of embodiment 2PEMC

[0101] 1 information

[0102] 1.1 General information

[0103] 3 clinical patients. The first patient was a 19-year-old male with pulmonary metastasis of left femoral osteosarcoma and left pleural effusion (Enneking stage III); the second patient was a 23-year-old female with pulmonary metastasis of right humeral osteosarcoma and right pleural effusion (Enneking stage III); the third patient was a 25-year-old male diagnosed with pulmonary metastasis of pelvic osteosarcoma with right pleural effusion (Enneking stage III).

[0104] 1.2 Diagnostic criteria

[0105] Chest X-ray often presents as a solitary spherical lesion, generally less than 2 cm, mostly round, with smooth edges and light density, without obvious burrs and lobulation features.

[0106] 1.3 Inclusion criteria

[0107] (1) Meet the above diagnostic criteria for lung metastatic tumors;

[0108] (2) Between the ages of 18 and 60, male or female;

[0109] (3) ...

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Abstract

The invention relates to a tumor treatment method for patients with terminal malignant tumor lung metastasis with malignant pleural effusion. The method includes the steps: draining and collecting pleural effusion from cancerous hydrothorax appearing in a patient with lung metastasis, centrifuging and enriching, then separating out metastatic pleural effusion mononuclear cells (PEMC), adjusting the concentration of 5*10<5>/ml in pores of a 24-pore plate, placing in an incubator, amplifying the PEMC with combined application of peripheral blood mononuclear cells (PBMC) in healthy adults after irradiation with IL-2, and taking the PBMC after irradiation as trophocytes to stimulate the amplification and cytotoxicity tests of the PEMC. The invention also provides a use of the PEMC prepared bythe method in preparation of drugs for treating terminal malignant tumor lung metastasis with malignant pleural effusion. The advantages show that provided is a PEMC amplification method with higher efficiency and relatively convenient technology, the amplification efficiency reaches 1000 times, and high cytotoxicity killing effect is achieved.

Description

technical field [0001] The invention relates to the technical fields of immunology, molecular biology and cell biology, in particular to a preparation and expansion method of T cells with malignant tumor lung metastasis and malignant pleural effusion and its application. Background technique [0002] Malignant pleural effusion refers to the pleural effusion caused by the metastasis of malignant tumors in the pleura or other parts to the pleura. It is also a common and difficult to control late complication of malignant tumors. It grows rapidly and is often accompanied by chest tightness, shortness of breath, and palpitations. Symptoms, untimely treatment can cause respiratory and circulatory dysfunction, hypoalbuminemia, anemia, and even life-threatening in severe cases, affecting the quality of life of patients. The occurrence of malignant pleural effusion often indicates that the tumor has disseminated or has advanced to an advanced stage. Most of the tumors have been resi...

Claims

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Application Information

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IPC IPC(8): C12N5/0783A61K35/17A61P35/00
CPCA61K35/17A61P35/00C12N5/0636C12N2501/2302
Inventor 王崇任蔡郑东华莹奇孙伟孙梦熊傅泽泽沈嘉康
Owner SHANGHAI FIRST PEOPLES HOSPITAL
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