Attenuated virus for prevention of infectious bursal disease and use

A bursal disease and infectious technology, applied in the field of attenuating viruses, can solve the problems of bursal damage, changing the new strain of virus immunogenicity, and delay in vaccine development, so as to achieve the effect of preserving immunogenicity

Active Publication Date: 2018-12-04
ZHEJIANG FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, immunization is still the most important method to prevent and control IBD, but the developed IBD vaccine has the following problems: live vaccines can still cause certain damage to the bursa of Fabricius, affect the growth and development of chickens, and reduce the ratio of meat to feed; Under pressure, mutated strains continue to emerge, and vaccine development lags behind
[0005] At present, the existing attenuated strains all change or modify the amino acid sequence of the neutralizing antigen VP2 protein of the IBDV virus to achieve the purpose of attenuation. Possibility of recombination with other strains to produce new strains

Method used

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  • Attenuated virus for prevention of infectious bursal disease and use
  • Attenuated virus for prevention of infectious bursal disease and use
  • Attenuated virus for prevention of infectious bursal disease and use

Examples

Experimental program
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Embodiment 1

[0023] Embodiment 1, the acquisition of attenuated strain of IBDV:

[0024] The clinically isolated parental strain IBDV / wt (Genbank accession numbers: MF083701 and MF083702) was amplified on chicken embryonic fibroblast cell line DF-1, and its genomic RNA was extracted to construct an IBDV reverse genetics operating system. The nucleotide sequence encoding the Flag tag was fused to the downstream of the vp1 gene by genetic engineering, and the obtained plasmid was transfected into 293T cells for IBDV rescue, and the cell supernatant after 72 hours of transfection was passaged on DF-1 cells for 3 times Afterwards, the cells were lysed by repeated freezing and thawing three times, and the cell debris was removed by centrifugation, and finally the attenuated IBDV strain IBDV / 1F was obtained.

[0025] IBDV attenuated strain IBDV / 1F has been preserved, and the preservation information is as follows:

[0026] Deposit name: Infectious Bursal Disease Virus IBDV / 1F, depository unit: ...

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Abstract

The invention discloses an attenuated infectious bursal disease virus, which has a preservation name of: infectious bursal disease virus IBDV/1F, and is preserved in China Center for Type Culture Collection at Wuhan University in Wuhan, China. The preservation number is CCTCC NO: V201834, and the preservation date: July 3, 2018. The invention also provides the use of the attenuated infectious bursal disease virus in preparation of vaccines. The viral strain does not cause bursal damage in chickens and can induce high titer antibodies directed at infectious bursal disease virus. The vaccine canresist the attack of virulent strains and has no influence on the development of chickens.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a strain of attenuated virus capable of effectively preventing infectious bursal disease virus infection and its application. Background technique [0002] Chicken infectious bursal disease (Infectious Bursal Disease, IBD) is caused by Infectious Bursal Disease Virus (Infectious Bursal Disease Virus, IBDV), an acute and highly contagious infection that mainly harms 3-6 week-old chicks The disease mainly invades the central immune organ of sick chickens—the bursa of Fabricius. The main pathological features are inflammation, necrosis and atrophy of the bursa of Fabricius. The B lymphocytes in the bursa of Fabricius are severely damaged and lead to severe immunosuppression. [0003] The IBDV virion has no envelope and is icosahedrally symmetrical. The genome consists of two dsRNA segments, A and B, encoding five viral proteins, VP1-5, among which VP1, VP2 and VP3 are recognized structura...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/04A61K39/12A61P31/14C12R1/93
CPCA61K39/12A61K2039/552A61P31/14C12N7/00C12N2720/10021C12N2720/10034
Inventor 叶承金刘合宾王宇陈绵绵
Owner ZHEJIANG FORESTRY UNIVERSITY
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