Stem cell ointment used for treating skin damages, and preparation method thereof

A skin injury and stem cell technology, applied in the field of cell biology, can solve the problems that the survival rate of single cells affects the curative effect, the curative effect, ischemia and hypoxia, etc., to improve the quality of healing, accelerate wound healing, and promote regeneration.

Active Publication Date: 2018-12-07
UNIVERSITY OF MACAU
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  • Abstract
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  • Application Information

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Problems solved by technology

This method can partially improve wound healing, but because the low survival rate of single cells greatly affects the curative effect, how to improve the survival rate of cells after transplantation is the key to increasing the curative effect
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Method used

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  • Stem cell ointment used for treating skin damages, and preparation method thereof
  • Stem cell ointment used for treating skin damages, and preparation method thereof
  • Stem cell ointment used for treating skin damages, and preparation method thereof

Examples

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Effect test

Embodiment 1

[0050] The invention provides a preparation method of stem cell ointment for treating skin damage, and the preparation method comprises the following three steps;

[0051] Step 1, the step of forming stem cell clumps, under the condition of suspension culture, the cell adhesion factor cadherin is highly expressed on the surface of mesenchymal stem cells, so that the stem cells spontaneously aggregate and form clumps;

[0052] Step 2, the preparation step of stem cell ointment, mixing the non-biotoxic matrix into the active energy liquid medium to form an ointment, and placing the stem cell mass formed in step 1 in the ointment to form a stem cell ointment;

[0053] Step 3, the stem cell ointment product packaging and storage step, the stem cell ointment is packaged in a sterile plastic container at room temperature, and can be directly applied to the skin wound before use to exert the biological activity of mesenchymal stem cells and treat skin wounds .

[0054] Specifically,...

Embodiment 2

[0077] The preparation method and steps of this example are basically the same as those of Example 1, except that in the step of forming stem cell clumps, U / V type ultra-low adsorption culture plates are used to cultivate stem cell clumps. The number of stem cells prepares uniform spheres of cell agglomerates of different sizes and specifications. The stem cell agglomerates prepared by U / V ultra-low adsorption culture plates or hanging drop method or glass culture bottle method are mainly due to cost, different size and uniformity, survival rate and There is no significant difference in biological activity; and in the trypsin digestion substep in the stem cell ointment preparation step described in Example 1, the trypsin is replaced by 0.05-0.5% collagenase, and the following will apply the method of the present invention to produce The curative effect of the stem cell ointment on immunodeficiency mice skin injury is described, specifically,

[0078] The stem cell ointment of ...

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PUM

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Abstract

The invention provides a stem cell ointment used for treating skin damages, and a preparation method thereof. The preparation method comprises following steps: mesenchymal stem cells with immunity adjusting and tissue regeneration promoting functions are prepared into blocks with uniform sizes under 3D suspension culture conditions, and are mixed with a biotoxicity-free active energy matrix so asto obtain the ointment. Stem cells are capable of living for as long as one week in the ointment at room temperature, and the biological activity is maintained. The stem cell ointment can be smeared onto skin damages directly, the stem cells in the ointment are capable of migrating into the wounds to inhibite inflammation, promote tissue regeneration, accelerate wound healing, improve healing status, and reducing scars; after restoration, the stem cells are eliminated by self immunity systems, no residual is left, the stem cell ointment is safe and stable, and is high in efficiency.

Description

technical field [0001] The invention relates to the field of cell biology, in particular to mixing stem cells with biologically active functions with a non-biotoxic matrix to make an ointment and a preparation method for the ointment. Background technique [0002] Acute and chronic wounds, such as various ulcers, ischemia-hypoxic wounds and burn residual wounds, endanger human health and affect the quality of life, and have become a huge problem in modern society. Due to the sharp increase in obesity, diabetes, and the aging population, the incidence of various skin injuries, especially chronic wounds, continues to rise. The treatment of these wounds consumes significant socioeconomic resources. The current means of wound treatment mainly include antibiotic intervention, surgery and negative pressure therapy. Although these treatments have made great progress, the healing rate of chronic wounds is still less than 50%. In the past two decades, stem cell research has made gr...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61K9/06A61K47/38A61P17/02C12N5/0775
CPCA61K47/38A61K9/0014A61K9/06A61K35/28C12N5/0663C12N5/0665C12N5/0667C12N2513/00
Inventor 王晓燕蒋斌徐仁和
Owner UNIVERSITY OF MACAU
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