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Cage structure microporous culture dish for forming three-dimensional microtissue of cells in vitro and preparation method thereof

A culture dish and micro-tissue technology, applied in the field of in vitro three-dimensional culture of biological cells, can solve the problems of cumbersome process, limited biological materials, high price, etc., and achieve the effects of simplifying the preparation process, simple preparation process and cost saving.

Active Publication Date: 2021-10-08
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in these three-dimensional cell culture models in vitro, the former requires the construction of cell scaffolds, the process is cumbersome, the applicable biological materials are limited, and the price is expensive; the cell microstructure produced by the hanging drop method is not easy to manipulate, and it is difficult to apply to more complex organisms. Therefore, the development of a simple model for the formation of a new type of three-dimensional microtissue in vitro has become one of the urgent problems to be solved.

Method used

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  • Cage structure microporous culture dish for forming three-dimensional microtissue of cells in vitro and preparation method thereof
  • Cage structure microporous culture dish for forming three-dimensional microtissue of cells in vitro and preparation method thereof
  • Cage structure microporous culture dish for forming three-dimensional microtissue of cells in vitro and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] In this example, see Figure 1 ~ Figure 3 , a cage structure microporous culture dish for forming three-dimensional cell microtissues in vitro, the cage structure microporous culture dish is composed of four basic parts spliced ​​and assembled:

[0044] The first basic part is the petri dish 4 after the bottom surface is modified, that is, the bottom of the petri dish 4 is combined with a biocompatible material film layer to form the inner layer of the petri dish 4 container, and the inner surface of the petri dish 4 is modified, see figure 1 ;

[0045] The second basic part is the modified polymer microwell array component 3, which adopts a biocompatible material film with a hollow micropore array structure, so that the polymer microwell array component 3 has cell growth micropore grooves distributed in an array , the size of the outer edge of the polymer microwell array assembly 3 is smaller than the size of the inner cavity of the container of the petri dish 4, and ...

Embodiment 2

[0064] This embodiment is basically the same as Embodiment 1, especially in that:

[0065] In this example, see Figure 4 , a cage-structured microporous culture dish for forming three-dimensional cell microtissues in vitro and its preparation method are the same as in Example 1.

[0066] Experimental test analysis:

[0067] The cage-structured microporous culture dish used in this embodiment for the formation of three-dimensional cell microtissues in vitro was used to conduct cell culture experiments in vitro. Utilize present embodiment to prepare cage structure microporous culture dish, its cell mesh 2 is the nylon mesh of pore 100 microns, as Figure 4 As indicated, the research on three-dimensional microtissue formation of lung tumor cells in vitro was carried out. In the experiment, 1×10 5 Individual / mL large cell carcinoma NCI-H460 cells in lung non-small cell lung cancer are placed in a cage-structured microwell culture dish, and the cells enter the cage-structured po...

Embodiment 3

[0070] This embodiment is basically the same as the previous embodiment, and the special features are:

[0071] In this example, see Figure 5 , a cage-structured microporous culture dish for forming three-dimensional cell microtissues in vitro and its preparation method are the same as in Example 1.

[0072] Experimental test analysis:

[0073] The cage-structured microporous culture dish used in this embodiment for the formation of three-dimensional cell microtissues in vitro was used to conduct cell culture experiments in vitro. Utilize present embodiment to prepare cage structure microporous culture dish, its cell mesh 2 is the nylon mesh of pore 100 microns, as Figure 5 As shown, a three-dimensional co-culture study of lung tumor cells and vascular endothelial cells was carried out. In the experiment, inoculate 1×10 5 Large cell carcinoma NCI-H460 cells / mL of lung non-small cell lung cancer were cultured in a cage microwell culture dish for 3 days to obtain NCI-H460 ...

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Abstract

The invention discloses a cage structure microporous culture dish for forming three-dimensional microtissues of cells in vitro and a preparation method thereof. The cage structure microporous culture dish is composed of four basic parts, and the first basic part is a modified bottom surface. Petri dish, the second basic part is the modified polymer microwell array, the third basic part is the cell screen, and the fourth basic part is the cell culture medium storage pool; the cage structure microporous culture dish can realize cell Inoculation, long-term cell culture, three-dimensional micro-tissue formation, online detection, multi-cell three-dimensional co-culture. The process of the invention is simple, easy to control and low in cost. When the cage structure microporous culture dish of the present invention is used for biological experiments, reagents are saved, the throughput is high, the operation is easy, and the three-dimensional culture of cells in vitro has high efficiency and good quality.

Description

technical field [0001] The invention relates to a three-dimensional micro-tissue formation model in vitro and a preparation method thereof, in particular to a culture dish device for three-dimensional micro-tissue formation in vitro and a preparation method thereof, which are applied in the technical field of three-dimensional in vitro culture of biological cells. Background technique [0002] The in vitro culture mode of cells can be simply divided into two-dimensional culture and three-dimensional culture, among which the more traditional and easy to implement is the two-dimensional culture of cells in vitro, such as well plates, culture flasks, dishes, etc., although they have significant value in biomedical research , but this model cannot fully simulate the cell microenvironment with complex biochemical and biophysical factors, and it is also difficult to reflect the complex physiological or pathological environment of human tissues and organs, which has considerable lim...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/22C12M1/26
CPCC12M23/10C12M33/02
Inventor 高兴华李开艳杨兴远
Owner SHANGHAI UNIV
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