A kind of extracellular matrix composite film with photothermal effect and preparation method thereof

A technology of composite film and photothermal effect, which is applied in the direction of medical preparations containing active ingredients, pharmaceutical formulas, drug combinations, etc., can solve problems affecting cell proliferation and differentiation, changes in the ultrastructure of extracellular matrix, etc., and achieve good photoluminescence. Thermal effect, good light and heat characteristics, the effect of avoiding direct contact

Active Publication Date: 2020-10-16
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, simple mechanical peeling will lead to certain changes in the ultrastructure of the extracellular matrix, which in turn will affect its subsequent effect on the proliferation and differentiation of other cells.

Method used

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  • A kind of extracellular matrix composite film with photothermal effect and preparation method thereof
  • A kind of extracellular matrix composite film with photothermal effect and preparation method thereof
  • A kind of extracellular matrix composite film with photothermal effect and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1). Sterilize the cell culture surface on which the visible light-induced cell detachment is performed by autoclaving;

[0028] (2). The cells previously cultured in the culture flask were detached, centrifuged at 1000r / min for 4min, suspended in high-sugar DMEM medium, and counted with a counting plate at 1×10 5 piece / cm 2 The cell density was seeded on the above-mentioned treated cell culture surface, and placed in a constant temperature of 37 ° C, 5% CO 2 Cultured in a cell incubator, the medium was changed once every 2 days, and the culture period was 9 days. In the last day of the culture period, 100 μg / mL nano-graphene particles with a particle diameter of 10 nm were added to form a complete cell culture surface. cell sheet;

[0029] (3). The cultured surface after the above culture was washed with PBS, then soaked in deionized water and then freeze-dried to obtain a cultured surface loaded with an extracellular matrix composite film, and the surface was steril...

Embodiment 2

[0035] (1). Sterilize the cell culture surface on which the visible light-induced cell detachment is performed by autoclaving;

[0036] (2). The cells previously cultured in the culture flask were detached, centrifuged at 800r / min for 6min, suspended in high-sugar DMEM medium, and counted with a counting plate at 2×10 5 piece / cm 2 The cell density was seeded on the above-mentioned treated cell culture surface, and placed in a constant temperature of 37 ° C, 5% CO 2 Cultured in a cell incubator, the medium was changed once every 2 days, and the culture period was 8 days. 90 μg / mL nano-graphene particles with a particle diameter of 15 nm were added on the second-to-last day of the culture period, and finally formed on the surface of the cell culture. the cell sheet;

[0037] (3). The cultured surface after the above culture was washed with PBS, then soaked in deionized water and then freeze-dried to obtain a cultured surface loaded with an extracellular matrix composite film, ...

Embodiment 3

[0042] (1). Sterilize the cell culture surface on which the visible light-induced cell detachment is performed by autoclaving;

[0043] (2). The cells previously cultured in the culture flask were detached, centrifuged at 900r / min for 5min, suspended in high-sugar DMEM medium, and counted with a counting plate at 5×10 5 piece / cm 2 The cell density was seeded on the above-mentioned treated cell culture surface, and placed in a constant temperature of 37 ° C, 5% CO 2 Cultured in a cell culture box, the medium was changed once every 2 days, and the culture period was 7 days. 80 μg / mL nano-graphene particles with a particle diameter of 20 nm were added on the penultimate day of the culture period, and finally formed on the surface of the cell culture. the cell sheet;

[0044] (3). The cultured surface after the above culture was washed with PBS, then soaked in deionized water and then freeze-dried to obtain a cultured surface loaded with an extracellular matrix composite film, a...

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Abstract

The invention discloses an extracellular matrix composite film with a photothermal effect and a preparation method of the extracellular matrix composite film with the photothermal effect. The method comprises the following steps: culturing cells on a cell culture surface for visible light-induced cell desorption, and adding nanoparticles having the photothermal effect during the culture process; after forming a cell lamella, washing and lyophilizing, then culturing the cells on the cell lamella again to form a cell lamella, obtaining a complete cell lamella by light-induced cell desorption, washing and decellularizing to obtain the extracellular matrix composite film having the photothermal effect. The composite film finally obtained by the method disclosed by the invention has the photothermal effect and is formed by stacking extracellular matrixes, and the composite nanoparticles are completely covered in the composite film, thereby avoiding direct exposure of the particles to affectthe cell compatibility of the film, and the composite film can be applied to bone tissue defect repair engineering and other fields. In addition, the preparation method disclosed by the invention hasa simple process, is easy to implement, and is favorable for popularization and application.

Description

technical field [0001] The invention relates to the field of bone tissue defect repair engineering, in particular to an extracellular matrix composite film with photothermal effect and a preparation method thereof. Background technique [0002] Cells in tissues and organs are surrounded by extracellular matrix, which is composed of various proteins and proteoglycans, which not only provide support and protection for cell growth, but more importantly, interact with cells to regulate the morphogenetic process of cells, affecting Cell survival, migration, proliferation and functional metabolism. Studies have shown that mild heat stimulation induces the early differentiation of human mesenchymal stem cells and enhances the maturation of osteoblasts differentiated from human mesenchymal stem cells, mainly manifested in the enhancement of alkaline phosphatase activity in the early stage of differentiation, and the activation of bone-specific genes in the middle and late stages of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/50A61K41/00A61P19/08
CPCA61K41/0052A61L27/3633A61L27/365A61L27/3691A61L27/50A61L2300/412A61L2400/12A61L2430/02A61L2430/40A61P19/08
Inventor 程逵孙媛翁文剑
Owner ZHEJIANG UNIV
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