High-quality oligomeric proanthocyanidins and preparation method thereof

A technology of oligomeric proanthocyanidins and proanthocyanidins, which is applied in the direction of organic chemistry, etc., can solve the problems of high concentration of hydrogen peroxide, high molecular weight, difficult to absorb, etc., and achieves the effects of wide demand, simple operation and strong anti-oxidation.

Active Publication Date: 2018-12-14
HUNAN DASANXIANG TEA OIL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This patent has the following disadvantages: no reduction polymerization is used, the degree of polymerization of proanthocyanidins is high, the molecular weight is high, it is not easy to be absorbed by the human body and human microorganisms, and the antioxidant capacity is weak
This patent has the following disadvantages: the concentration of hydrogen peroxide is high, and the amount of hydrogen peroxide added is large, it is easy to oxidize the oligomeric proanthocyanidins just produced, and the yield of oligomeric proanthocyanidins is low
A large amount of water is used to extract raw materials at high temperature, which increases production costs, and the yield of oligomeric proanthocyanidins is low, and it is difficult to obtain high-purity proanthocyanidins

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039]Take 200 grams of crushed grape seeds, add 1000mL of 39% ethanol solution, extract at 50°C for 90 minutes, filter and concentrate the extract to obtain a concentrated solution, put the concentrated solution on HPD-100 macroporous adsorption resin, the flow rate of the upper column is 3BV / h, and the upper The sample flow rate is 5BV / h, first wash the column with distilled water, the water washing flow rate is 3BV / h, let the adsorption time stand for 1 hour, use 39% alcohol aqueous solution as the eluent, the elution flow rate is 3BV / h, the eluent dosage is 3BV, collect the alcohol eluent. Concentrate the ethanol eluate until it has no alcohol smell, then adjust the concentration of proanthocyanidins to 5 mg / mL with water, adjust the pH of the proanthocyanidin solution to 9.0 with sodium carbonate solution, add 0.5% ozone of the mass of the proanthocyanidin solution, and reduce the concentration of proanthocyanidin at 20°C for 30 minutes. The polymerized proanthocyanidin s...

Embodiment 2

[0041] Take 200 grams of crushed pine bark, add 1400mL30% ethanol solution, extract at 59°C for 60 minutes, filter and concentrate the extract to obtain a concentrated solution, apply the concentrated solution to HPD-100 macroporous adsorption resin, and the flow rate of the upper column is 3BV / h. The sample flow rate is 5BV / h, first wash the column with distilled water, the water washing flow rate is 2BV / h, let the adsorption time stand for 2 hours, use 20% alcohol aqueous solution as the eluent, the elution flow rate is 3BV / h, the eluent dosage is 5BV, collect the alcohol eluent. Concentrate the alcohol eluent until it has no alcohol smell, then adjust the concentration of proanthocyanidins to 8 mg / mL with water, adjust the pH of the proanthocyanidin solution to 8.0 with sodium bicarbonate solution, add 0.3% ozone of the mass of the proanthocyanidin solution, and polymerize at 25°C for 20 minutes. The depolymerized proanthocyanidin solution is spray-dried to obtain oligomeri...

Embodiment 3

[0043] Take 200 grams of crushed tea seed shells, add 1600mL of 30% ethanol solution, extract at 40°C for 120 minutes, filter and concentrate the extract to obtain a concentrated solution, put the concentrated solution on D101 macroporous adsorption resin, the column flow rate is 3BV / h, and load the sample The flow rate is 5BV / h, first wash the column with distilled water, the flow rate of water washing is 3BV / h, and the adsorption time is 0.5 hours. Use 30% alcohol aqueous solution as the eluent, the elution flow rate is 3BV / h, the amount of eluent is 4.5BV, and the alcohol is collected eluent. Concentrate the alcohol eluate until it has no alcohol smell, then adjust the concentration of proanthocyanidins to 10 mg / mL with water, adjust the pH of the proanthocyanidin solution to 8.5 with sodium hydroxide solution, add 1% ozone of the mass of the proanthocyanidin solution, and polymerize at 20°C for 40 minutes. The depolymerized proanthocyanidin solution is spray-dried to obtai...

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Abstract

The invention relates to the technical field of natural plant extraction processing, and concretely relates to a high-quality oligomeric proanthocyanidins and a preparation method thereof. The high-quality oligomeric proanthocyanidins can be obtained by the steps of extraction, concentration, purification, re-concentration, water dissolution, depolymerization, and spray drying. The raw materials and auxiliary materials selected by the method are natural materials, the method employs physical synthesis and biological fermentation methods, the oligomeric proanthocyanidins can be completely usedfor food processing and is safe and quality-assured, the operation is simple, the prepared oligomeric proanthocyanidins has strong anti-oxidizability.

Description

technical field [0001] The invention relates to the technical field of natural plant extraction and processing, in particular to a method for preparing high-quality oligomeric proanthocyanidins. technical background [0002] Proanthocyanidins are polyphenolic compounds of a class of flavanol monomers and their polymers, also known as condensed tannins. Proanthocyanidin is a natural biologically active substance widely distributed in nature. It has the functions of regulating immunity and anti-inflammation, protecting liver and lowering fat, and protecting intestinal damage. ] , prevention and treatment of atherosclerosis, anti-ultraviolet rays, protection of optic nerve, anti-oxidation and other pharmacological activities, it has many applications in the fields of food, health products, pharmaceuticals and cosmetics. [0003] Proanthocyanidins are polymers formed by the polymerization of catechins or / and epicatechins in different quantities. Generally speaking, proanthocyan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62
CPCC07D311/62
Inventor 赖琼玮陈劲松杨耀学邓一军凌小辉黄闺
Owner HUNAN DASANXIANG TEA OIL CO LTD
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