Production method of haemophilus parasuis/mycoplasma hyopneumoniae bivalent inactivated vaccine

A technology of Mycoplasma hyopneumoniae and double inactivated vaccine, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, vaccines, etc., can solve the problems of increasing labor and production costs, antigen interference or influence, and increasing pollution risks, etc. Achieve the effect of reducing labor costs, reducing electrical use costs, and reducing the risk of microbial contamination

Active Publication Date: 2018-12-18
WUHAN KEQIAN BIOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, due to combination vaccines (vaccine compositions), such as double vaccines or triple vaccines, multiple antigens interfere or affect each other, resulting in no dual vaccines for parasuis and mycoplasma pneumoniae currently on the market. The produc

Method used

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  • Production method of haemophilus parasuis/mycoplasma hyopneumoniae bivalent inactivated vaccine
  • Production method of haemophilus parasuis/mycoplasma hyopneumoniae bivalent inactivated vaccine
  • Production method of haemophilus parasuis/mycoplasma hyopneumoniae bivalent inactivated vaccine

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Embodiment 1

[0058] 1. Vaccine preparation

[0059] 1. Preparation of seed bacteria

[0060] (1) First-class seed propagation and identification

[0061] The freeze-dried bacteria of Haemophilus parasuis serotype 4 strain MD0322 and the freeze-dried strain of Haemophilus parasuis serotype 5 SH0165 were streaked and inoculated on TSA agar plates, and cultured at a constant temperature of 37 °C for 20-24 hours. Typical bacterial colonies or bacterial lawns are produced, and after passing the pure inspection, they are used as first-class seeds and stored at 2-8°C for no more than 7 days; freeze-dried strains of Mycoplasma hyopneumoniae XJ03 strain are inoculated with 100ml / bottle of Mycoplasma liquid culture medium medium, cultured at 37°C for 3 to 5 days, when the color of the medium turned yellow, slightly turbid, and the pH value dropped to 6.5-6.8, the bacterial liquid was harvested, and it was regarded as the first-class seed after passing the pure inspection. The specific parameters ar...

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Abstract

The invention belongs to the technical field of vaccine production, and particularly discloses a production method of a haemophilus parasuis/mycoplasma hyopneumoniae bivalent inactivated vaccine. Themethod includes: performing fermenting culture of mycoplasma hyopneumoniae in a bioreactor; performing fermenting culture of serum IV type haemophilus parasuis and serum V type haemophilus parasuis ina fermentation tank; inactivating, concentrating and purifying the microbial liquids after the fermenting cultivation, mixing the microbial liquids according to certain ratio, and adding an immuno-enhancer and a vaccine adjuvant to obtain the haemophilus parasuis/mycoplasma hyopneumoniae bivalent inactivated vaccine. The vaccine composition has excellent specificity and good immunity, can achievethe object of prevention from the two diseases by one injection, is more economical and practicability, can avoid repeated inoculation and reduces vaccine cost and manpower cost. The bivalent inactivated vaccine is very suitable for prevention and treatment on breeding farms with mixed infection of the diseases.

Description

technical field [0001] The invention belongs to the technical field of vaccine production, and in particular relates to the production of a dual inactivated vaccine of Haemophilus parasuis and Mycoplasma hyopneumoniae. Background technique [0002] Haemophilus parasuis has been one of the most important bacterial diseases among swine diseases in recent years, seriously affecting the healthy development of pig industry in my country. Haemophilus parasuis (HPS) can cause polyserositis, arthritis, and meningitis in pigs, which can affect suckling piglets from 2 weeks old to 4-month-old fattening pigs, mainly after weaning and nursery. Stage morbidity, more common in pigs of 5 to 8 weeks of age, the incidence rate is generally 10% to 15%, and the case fatality rate is as high as 50% when severe. The present invention analyzes all bacteria isolated from 2013 to 2017, and the results show that the isolation rate of Haemophilus parasuis reaches about 30.8%, among which the isolate...

Claims

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Application Information

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IPC IPC(8): A61K39/102A61K39/02A61P31/04C12N1/20C12R1/35
CPCA61K39/0241A61K39/102A61K2039/521A61K2039/70A61P31/04C12N1/20A61K2300/00
Inventor 徐高原陈波金建云周明光潘建刚卢强余蕾范金秀
Owner WUHAN KEQIAN BIOLOGY CO LTD
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