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Polynorepinephrine modified magnetic particle and preparation method and application thereof

A technology of noradrenaline and magnetic particles, applied in the preparation of test samples, chemical instruments and methods, and other chemical processes, to achieve good adsorption and elution, mild conditions, and good reproducibility

Active Publication Date: 2018-12-18
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These two methods are mainly directly applied to the purity detection of pharmaceutical raw materials or pharmaceutical preparations with relatively simple sample matrices, and are not suitable for direct analysis of low-concentration enantiomers of β-blockers in biological samples with complex matrices.

Method used

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  • Polynorepinephrine modified magnetic particle and preparation method and application thereof
  • Polynorepinephrine modified magnetic particle and preparation method and application thereof
  • Polynorepinephrine modified magnetic particle and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The preparation of polyNE-MNPs includes the following steps:

[0036] (1) Preparation of COOH-MNPs: 4mM FeCl 3 ·6H 2 O was added to 30mL of diethylene glycol and 10mL of ethylene glycol for ultrasonic dissolution, then 3g of anhydrous sodium acetate and 3g of sodium acrylate were added, and stirred in a constant temperature water bath at 70°C at a speed of 500r / min for 1h to form a homogeneous solution. Pour it into a Teflon high-pressure reactor, put it in an oven, heat up to 200°C, react for 10 hours, cool to room temperature, separate the product under an external magnetic field and wash it with ethanol and deionized water for 3 times, and dry it at 60°C Uniformly dispersed COOH-MNPs were obtained.

[0037] (2) 30 mg of norepinephrine was dissolved in Tris-HCl aqueous solution (10 mL, 50 mM, pH 8.5) to prepare a norepinephrine solution. Then, 25 mg COOH-MNPs were weighed and dispersed in the norepinephrine solution, and stirred at 40 °C for 12 h. The obtained pol...

Embodiment 2

[0045] The preparation of polyNE-MNPs includes the following steps:

[0046] (1) Preparation of COOH-MNPs: 5mM FeCl 3 ·6H 2 O was added to 20mL of diethylene glycol and 20mL of ethylene glycol for ultrasonic-assisted dissolution, then 6g of anhydrous sodium acetate and 3g of sodium acrylate were added, and stirred in a constant temperature water bath at 50°C at a speed of 300r / min for 1h to form a homogeneous solution. Pour it into a Teflon autoclave, put it in an oven, raise the temperature to 180°C, react for 15 hours, cool to room temperature, separate the product under an external magnetic field and wash it three times with de-ethanol and deionized water successively, and dry at 60°C Dry to obtain uniformly dispersed COOH-MNPs.

[0047] (2) 25 mg of norepinephrine was dissolved in Tris-HCl aqueous solution (10 mL, 30 mM, pH 7.5) to prepare a norepinephrine solution. Then, 25 mg COOH-MNPs were weighed and dispersed in the norepinephrine solution, and stirred at 50 °C for...

Embodiment 3

[0055] The preparation of polyNE-MNPs includes the following steps:

[0056] (1) Preparation of COOH-MNPs: 6mM FeCl 3 ·6H 2 O was added to 32mL of diethylene glycol and 8mL of ethylene glycol for ultrasonic-assisted dissolution, then 9g of anhydrous sodium acetate and 3g of sodium acrylate were added, and stirred in a constant temperature water bath at 60°C at a speed of 400r / min for 1h to form a homogeneous solution. Pour it into a Teflon autoclave, put it in an oven, raise the temperature to 190°C, react for 20 hours, cool to room temperature, separate the product under an external magnetic field and wash it three times with de-ethanol and deionized water successively, and dry at 60°C Dry to obtain uniformly dispersed COOH-MNPs.

[0057] (2) 50 mg of norepinephrine was dissolved in Tris-HCl aqueous solution (10 mL, 70 mM, pH 9.5) to prepare a norepinephrine solution. Then, 25 mg COOH-MNPs were weighed and dispersed in the norepinephrine solution, and stirred at 25 °C for ...

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Abstract

The invention discloses a polynorepinephrine modified magnetic particle and a preparation method and application thereof, and the preparation method comprises the steps of: dissolving ferric chloridein a mixed solvent of diethylene glycol and ethylene glycol, adding anhydrous sodium acetate and sodium acrylate to mix well to obtain a mixture, pouring the resulting mixture into a Teflon high pressure reactor, heating to 180-200 DEG C, reacting for 10-20 h to obtain COOH-MNPs; adding the synthesized COOH-MNPs to a norepinephrine base solution, and performing heating, stirring and separating toobtain polyNE-MNPs. The polyNE-MNPs have mild preparation conditions, simple operation, good reproducibility and stability, and can well adsorb and elute beta-receptor antagonists, and can realize separation and enrichment of the beta-receptor antagonists in diluted urine samples.

Description

technical field [0001] The invention belongs to the technical field of magnetic solid-phase extraction, in particular to the preparation of polynephrine-modified magnetic particles (polyNE-MNPs) and its application in the field of biomedicine, and is particularly suitable for the chirality of beta-receptor blockers. Analytical applications of enantiomers. Background technique [0002] β-blockers are a class of chiral drugs widely used clinically to treat cardiovascular diseases, and are commonly used in the treatment of hypertension, arrhythmia, angina pectoris and other related diseases. Most beta-blockers contain a stereoactive center, so each beta-blocker has 2 enantiomers. Studies have shown that different enantiomers of β-receptor blockers generally exhibit different physiological and toxicological activities, and there are huge differences in pharmacodynamics and pharmacological properties. [0003] Currently, methods for determining the enantiomers of β-blockers mai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/26B01J20/28B01J20/30G01N1/34G01N1/40
CPCB01J20/06B01J20/265B01J20/28009B01J20/28016B01J20/3085G01N1/34G01N1/405
Inventor 贾丽吴佳
Owner SOUTH CHINA NORMAL UNIVERSITY
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