Preparation method of ginsenoside enzymatic hydrolysate
A technology of ginsenosides and enzymatic hydrolysis products, which is applied in the field of preparation of ginsenosides enzymatic hydrolysis products, can solve the problems of complicated process, enzyme inactivation, and low product recovery rate, and achieve simple process, low cost, and reduced high-temperature enzyme removal The effect of steps
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[0019] The invention provides a preparation method of ginsenoside enzymatic hydrolysis product, comprising:
[0020] Step 1: reacting raw materials containing ginsenoside with ginsenoside degrading enzyme to obtain ginsenoside enzymatic hydrolysis reaction product;
[0021] Step 2: adding the ginsenoside enzymatic hydrolysis reaction product of step 1 into a solvent for precipitation, the precipitation temperature is preferably at room temperature, and the precipitation time is preferably more than 30 minutes, more preferably 30-60 minutes, to obtain the precipitation product;
[0022] Step 3: filtering the precipitated product obtained in step 2, and performing rotary evaporation on the obtained filtrate to obtain a rotary evaporation concentrate;
[0023] Step 4: Dry the concentrated solution obtained in Step 3 by rotary evaporation to obtain ginsenoside enzymatic hydrolysis products.
[0024] According to the present invention, the ginsenoside-containing raw material is re...
Embodiment 1
[0030] Step 1: Add 200 μL Thermotoga neapolitana DSM 4359 β-glucosidase enzyme solution to 200 μL pH 5.0 phosphate buffer, then add 1 g ginsenoside to make the final concentration of ginsenoside 5 mg / mL for reaction, and react at 85 ° C for 180 min to obtain ginsenoside Enzymatic hydrolysis reaction products;
[0031] Step 2: Add the ginsenoside enzymatic hydrolysis reaction product of Step 1 into 4 times ethanol for precipitation at room temperature for 1 hour to obtain the precipitated product;
[0032] Step 3: Filter the precipitated product obtained in Step 2, discard the solid, and recover the filtrate with a rotary evaporator, and rotary evaporate until it tastes free of organic solvents, and obtain a rotary evaporated concentrate;
[0033] Step 4: Spray-dry the concentrated solution obtained in Step 3 to obtain the enzymatic hydrolysis product of ginsenoside.
[0034] The experimental results show that the enzymatic hydrolysis product of ginsenoside prepared in Example...
Embodiment 2
[0036] Step 1: Add 200 μL Thermotoga neapolitana DSM 4359 β-glucosidase enzyme solution to 200 μL pH 5.0 phosphate buffer, then add 1 g ginsenoside to make the final concentration of ginsenoside 5 mg / mL for reaction, and react at 85 ° C for 180 min to obtain ginsenoside Enzymatic hydrolysis reaction products;
[0037] Step 2: adding the ginsenoside enzymatic hydrolysis reaction product of step 1 into 3 times ethanol for precipitation at room temperature for 1 hour to obtain the precipitated product;
[0038] Step 3: Filter the precipitated product obtained in Step 2, discard the solid, and recover the filtrate with a rotary evaporator, and rotary evaporate until it tastes free of organic solvents, and obtain a rotary evaporated concentrate;
[0039] Step 4: Spray-dry the concentrated solution obtained in Step 3 to obtain the enzymatic hydrolysis product of ginsenoside.
[0040] The experimental results show that: the ginsenoside enzymatic hydrolysis product prepared in Exampl...
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