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Cultivation culture medium, preparation method and factoryized strain bag cultivation method of phlebopus portentosus

A cultivation method and a culture medium technology, which are applied in the field of industrialized bacterial bag cultivation and cultivation medium of Dictyostelium bruceae, can solve the problems of high pollution rate, long covering time, and slow growth of boletus mycelium and other issues to achieve the effect of reducing pollution rate and saving cost

Pending Publication Date: 2018-12-21
周茂
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Boletus phelicus belongs to high-temperature mushrooms, and its culture conditions are similar to the optimum conditions of many molds. Moreover, the growth rate of boletus mycelium is slow, and the initial covering time is long, resulting in a high pollution rate. The current cultivation methods are as follows: Ignoring this problem leads to high pollution rate, and some use purification culture in the whole process, resulting in high cost and high energy consumption, which is not conducive to commercial production
In addition, the existing culture media are all developed in the direction of complete saprophytic growth. Dictyostelium bursa is an ectomycorrhizal fungus with saprophytic properties. , cellulase and other decomposed raw materials have low biotransformation rate, mycelium is sparse, pollution and production capacity are affected to a certain extent
Therefore, there is an urgent need for a low-cost, low-pollution, high-efficiency cultivation method, and a culture medium that is more suitable for the growth of Dictyostelium punctatus, to solve the current need for large-scale, industrialized cultivation of Dictyostelium punctatus. liver bacteria problem

Method used

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  • Cultivation culture medium, preparation method and factoryized strain bag cultivation method of phlebopus portentosus
  • Cultivation culture medium, preparation method and factoryized strain bag cultivation method of phlebopus portentosus

Examples

Experimental program
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Embodiment 1

[0037] A culture medium, comprising raw materials and the mass percentage of raw materials: 15% fine wood chips, 15% corncobs, 3% bran, 1% gypsum powder, 3% molasses, 0.5% white sugar, KH 2 PO 4 0.1%, MgSO 4 0.1%, yeast extract 0.2%, balance water, pH 5.5.

[0038] A kind of method of utilizing the culture medium to carry out the factory-made bacteria bag cultivation method of reticulum phalaenopsis boletus, comprises the following steps:

[0039] (1) preparing the cultivation medium;

[0040] (2) Cultivation medium bagging and sterilization: the cultivation medium prepared in step (1) is sub-packed in bacteria bags, and then sterilized after sealing;

[0041] (3) Inoculation and bacteria cultivation: inoculate the liquid strains cultivated in the fermenter into the cultivated bacteria bags sterilized in step (2), and insert 30-50 ml of strains into each bag, and then carry out stage-by-stage cultivation. Bacterial culture:

[0042] (4) Covering soil, covering soil cultiva...

Embodiment 2

[0045] Compared with Example 1, the difference is that the composition ratio of the cultivation medium is different: bagasse 20%, corn cob 20%, bran 5%, gypsum powder 3%, molasses 5%, white sugar 1.5%, KH 2 PO 4 0.3%, MgSO 4 0.3%, yeast extract 0.6%, the balance is water, pH6.5.

Embodiment 3

[0047] Compared with Example 1, the difference is that the composition ratio of the cultivation medium is different: fine sawdust and straw 17%, corn cob 17%, bran 4%, gypsum powder 2%, molasses 4%, white sugar 1%, KH 2 PO 4 0.2%, MgSO 4 0.2%, yeast extract 0.5%, the balance is water, PH6.

[0048] The moisture content of the soil-containing medium cannot be higher than 51%, otherwise there will be mud, which will affect the ventilation and high viscosity will cause trouble for mechanized charging. The cultivation medium provided by the invention not only has sufficient nutrition and is easy to absorb, but also solves some problems of low water content of the substrate and actual mechanized loading. In addition, the water content of the culture medium is easy to control accurately, and the consistency between batches is good, so it is suitable for industrial cultivation.

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Abstract

The invention relates to a cultivation culture medium and a preparation method. The culture medium is adopted for culture of mycelia, the mycelia can grow on entire strain bags in 25 days, the yield is up to 150 g, and the cultivation culture medium and the preparation method are more suitable for use in mechanized planting. The invention also relates to a factoryized strain bag cultivation methodof phlebopus portentosus. The factoryized strain bag cultivation method of phlebopus portentosus includes the steps of preparation of the culture medium, bagging, sterilization, inoculation, cultureof the mycelia, soil covering, soil covering culture and culture of fruited mushrooms. Phlebopus portentosu is subjected to fine culture in stages, the pollution rate can be effectively decreased, the cost can be effectively reduced, rapid growth of the mycelia is ensured, high vitality is achieved, the production stability is improved, and the yield is increased; meanwhile, a mushroom fruiting method in a greenhouse is provided, mushroom fruiting can be achieved under simple conditions, the production threshold of phlebopus portentosus is reduced, and the cultivation culture medium, the preparation method and the factoryized strain bag cultivation method of phlebopus portentosus are suitable for large-scale popularization.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a cultivation medium, a preparation method of the medium and a method for industrialized fungus bag cultivation of Dictyostelium bursa. Background technique [0002] Phelebopus portentosus, also known as black boletus, is a rare edible fungus, which is delicious and nutritious, and has a broad market prospect as an edible fungus. However, the number of such bacteria that grow naturally is very limited. In order to meet the needs of consumers for Dictyostelium bruceae, it is necessary to develop an artificial cultivation method. With the development of research on the cultivation and planting technology of Dictyostelium bruceae in recent years, medium-scale cultivation and planting with bottle planting methods have been realized, and great progress has been made in understory bionic cultivation, but it is not yet suitable for large-scale cultivation. , The planting method of commerci...

Claims

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Application Information

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IPC IPC(8): A01G18/00A01G18/20
CPCA01G18/00A01G18/20
Inventor 周茂李庆唐凯唐林
Owner 周茂
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