Establishment of conversion model of intracranial hemorrhage of dog after autologous thrombus embolic infarction thrombolysis by using intervention method and application thereof

Abstract

The invention relates to an establishment of a conversion model of the intracranial hemorrhage of a dog after autologous thrombus embolic infarction thrombolysis by using an intervention method and anapplication thereof. The method comprises the following steps of: preparation of thrombus, preparation of a cerebral infarction model, thrombolysis and determination of hemorrhage result. The dog hemorrhage conversion model has the advantages that: first, the cerebral structure of a beagle is similar to that of a human being, the variation of the cerebral vascular diameter is small, compared withsmall animals, such as a mouse and the like, the related experimental result can achieve better clinical conversion. Second, in small animal experiments, a laser Doppler blood flow meter is used to monitor the changes of cerebral blood flow, thus judging the occlusion and recanalization of blood vessels. DSA is a gold standard for judging whether the blood flow is smooth or not, the injection ofthe thrombus can be guided, accurate embolism can be carried out, and direct evidence for judging whether the thrombolysis is valid or not is provided. Thirdly, an intervention procedure is used as aminimally invasive technique, other unnecessary operation damage can be reduced to the utmost extent, so that the accuracy and reliability of the experimental result can be improved.

Description

technical field [0001] The invention belongs to the technical field of basic medicine, and specifically relates to an interventional method for establishing a canine autologous thromboembolic cerebral infarction intracranial hemorrhage transformation model after thrombolysis and its application. Background technique [0002] In recent years, acute cerebral infarction has seriously affected human health, and about 10 to 40% of patients after cerebral infarction will naturally transform into intracranial hemorrhage, which further increases the mortality and disability rates. With the gradual increase in clinical application of intravenous injection of tissue plasminogen activator (rtPA) and transvascular thrombectomy in the treatment of acute cerebral infarction, these treatment methods have also increased blood perfusion in ischemic brain tissue. The proportion of intracranial hemorrhage transformation occurred. Especially in thrombolytic therapy, if the blood perfusion of i...

AI Technical Summary

Problems solved by technology

At present, some progress has been made in the experimental research on the correlation of intracranial hemorrhage after thrombolysis in cerebral infarction, but all small animals such as mice are used as experimental animals. The relevant experimental results cannot achieve effective clinical transformation

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