Preparation method of microorganism inoculant for domestic waste
A technology of domestic garbage and fermented bacterial agents, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of adverse effects of the surrounding environment, low fermentation degradation efficiency, long fermentation fermentation time, etc., and achieve maintenance The effects of high composting temperature, improved fermentation degradation efficiency, and short fermentation cycle
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[0020] Take 15-24g glucose, 5-10g peptone, 10-12g yeast extract, 2-3g beef extract, 3-5g sodium chloride, 1-2g triammonium citrate, 1-3g ammonium chloride, 0.5-0.6g phosphoric acid Dipotassium hydrogen, add 1.0~1.2L deionized water, stir at 300~400r / min for 20~30min, then use 10% sodium bicarbonate solution to adjust the pH to 6.5~7.5, and put it into an autoclave , Sterilize at 120-125°C for 20-30 minutes, then add 120-200g of landfill leachate, mix evenly to obtain a medium, use an inoculation loop to select 1-2 rings of Frankia, and inoculate in 100-120mL of liquid medium and placed in a shaker incubator, shake culture at 120-130r / min at 28-32°C for 3-4 days, then transfer to a centrifuge, and centrifuge at 3000-5000r / min to obtain a domestic waste fermentation agent. Add the stock solution of domestic waste fermentation bacteria agent into deionized water to dilute 10-100 times and then spray it directly on the surface of domestic waste. Spray 300-500g of liquid bacterial ...
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