A method for screening probiotics that achieve antidepressant function through 5-hydroxytryptophan-related pathway based on cellular level

A hydroxytryptophan, cell-level technology, applied in biochemical equipment and methods, biological testing, microbial determination/inspection, etc., can solve problems such as unexplainable, high cost, and long time-consuming
CN109055583BActive Publication Date: 2022-02-01JIANGNAN UNIV
6 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2022-02-01

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a method for screening probiotics that realize antidepressant function through 5-hydroxytryptophan-related pathways based on cell level. Using RIN14B cells as a screening model, through the co-incubation of probiotics and cells, the secretion of 5-hydroxytryptophan in the cell supernatant and the mRNA level of tryptophan hydroxylase 1 in the cells were detected to determine the ability to pass 5-hydroxytryptophan Hydroxytryptophan probiotics exert antidepressant effects. The effective bacterial strain screened by the present invention can alleviate the depression-like behavior of depressed mice, improve the levels of neurotransmitters and neurotrophic factors in the brain, and reduce the hyperfunction of the hypothalamus-pituitary-adrenal axis caused by stress, and the results of cell model screening are consistent with those of animals. The experimental results have a significant correlation. The antidepressant potential of different bacterial strains can be measured in batches in a short time by using the invention, and a more rapid and efficient method is provided for the screening of probiotics with antidepressant function.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the technical field of probiotic screening, in particular to a cell-based screening method for probiotics that achieve antidepressant functions through 5-hydroxytryptophan-related pathways. Background technique

[0002] Depression, also known as depressive disorder, is characterized by significant and persistent depression as the main clinical feature. Currently, more than 350 million people worldwide suffer from depression, and it is estimated that by 2030, depression will rank first in the global disease burden. A large number of studies have shown that the occurrence of depression is related to the imbalance of monoamine neurotransmitters in the brain, mainly manifested as a decrease in the concentration of 5-hydroxytryptamine (5-HT) in the synaptic gap of the brain. The current treatment for depression mainly focuses on increasing the level of 5-HT in the brain, such as selective serotonin reuptake inhibitors (SSRIs), the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More