A method for analyzing the effect of arbuscular mycorrhizal fungi on plant water use efficiency
A technology for arbuscular mycorrhizal fungi and plants, applied in the field of botany, can solve problems such as unclearness, and achieve the effect of improving plant water use efficiency
Active Publication Date: 2021-01-26
GUIZHOU SERICULTURE RES INST GUIZHOU PEPPER RES INST
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Problems solved by technology
However, whether arbuscular mycorrhizal fungi increase plant WUE by promoting plant growth, regulating root-to-shoot ratio, leaf photosynthetic rate or transpiration rate, or promoting nutrient uptake is still unclear.
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Embodiment 1
[0018] In the first step, after the required potting soil is collected, 10KGy of cobalt-60 is used to sterilize the soil, and the sterilized soil is packed into plastic buckets, each bucket is filled with 5.0kg of sterilized soil, and arbuscular bacteria are applied. Root fungal agent 50g.
[0019] The second step is to select two mulberry seedlings with the same growth and move them into the sterilized soil plastic bucket containing the fungicide.
[0020] In the third step, during the inoculation and cultivation of mulberry seedlings, all treatments are supplied with normal water, and 200mL / barrel of sterilized water is regularly poured several times a week, the growth temperature is 25-28°C, and the light time is 8-10h / d. After the mulberry tree was cultured for 40 days to form mycorrhiza, no water was supplied, and it was naturally dry for 7 days; then, parameters such as plant height, ground diameter, dry weight of the aboveground part, dry weight of the root, mycorrhizal...
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The invention discloses a method for analyzing the influence of arbuscular mycorrhizal fungi on plant water use efficiency, comprising the following steps: applying a certain amount of arbuscular mycorrhizal fungi after soil sterilization; transplanting or sowing target plants; After growing for a period of time, stop water supply for more than 7 days, start monitoring various growth indicators, calculate the growth status, root-to-shoot ratio, nutrient and water use efficiency of the target plant, and use SPSS to save the data in a certain format in .sav format; use AMOS The software builds a structural equation model based on the relationship between the independent variables and the relationship between the independent variable and the dependent variable. According to the calculation results of the structural equation model, the reasons for the influence of the inoculation of arbuscular mycorrhizal fungi on the water use efficiency of plants are explained. The present invention adopts the structural equation model analysis method, which can well explain why the water use efficiency of plants is improved, and further adopts artificial control measures for vegetation restoration in arid areas to more effectively improve the water use efficiency of plants and bring obvious effects.
Description
technical field [0001] The invention relates to the field of botany, in particular to a method for analyzing the influence of arbuscular mycorrhizal fungi on plant water use efficiency. Background technique [0002] Water is the main factor limiting plant survival and ecological adaptability. Reasonable and effective use of limited water resources is the prerequisite for long-term stable survival of plants, adaptation to harsh environments, and vegetation restoration and reconstruction. Plant water use efficiency (WUE) can reflect the water use strategy of plants, and is an important physiological and ecological parameter to evaluate the suitability of plants to adversity and measure the drought tolerance of plants. Improving WUE is an urgent problem to be solved in plant production. An important strategic measure for the sustainable development of regional plants. [0003] Arbuscular mycorrhizal fungi can help plants effectively use soil water and nutrients, promote their ...
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IPC IPC(8): G01N33/00
CPCG01N33/0098
Inventor 邢丹杨万荣毛明明罗恒韩世玉张芳
Owner GUIZHOU SERICULTURE RES INST GUIZHOU PEPPER RES INST