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Method for extracting and separating mulberry red pigments by liquid-liquid-solid system enzymatic hydrolysis

A technology that catalyzes hydrolysis and red pigment, which is applied in fermentation and other directions, to achieve the effects of environmental friendliness, increased production, and easy industrial application

Inactive Publication Date: 2018-12-25
JIANGSU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report about the enzymatic hydrolysis-extraction of ethanol-salt-immobilized enzyme liquid-liquid-solid system for the separation of mulberry red pigment

Method used

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  • Method for extracting and separating mulberry red pigments by liquid-liquid-solid system enzymatic hydrolysis
  • Method for extracting and separating mulberry red pigments by liquid-liquid-solid system enzymatic hydrolysis
  • Method for extracting and separating mulberry red pigments by liquid-liquid-solid system enzymatic hydrolysis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Take free α-L-rhamnosidase and 9 kinds of immobilized materials: multi-walled carbon nanotubes, carboxylated multi-walled carbon nanotubes, hydroxyl multi-walled carbon nanotubes, short carboxylated multi-walled carbon nanotubes, short multi-walled carbon nanotubes Walled carbon nanotubes, carboxylated carbon nanotubes, carbon nanotube nickel, short hydroxylated multi-walled carbon nanotubes, and graphene were added to the two-phase aqueous system at a ratio of 5wt.% to form a three-phase system, at pH 4.5 , and reacted for 1 h at a temperature of 35°C. After the reaction, centrifuge and stand still, take samples from the upper and lower phases respectively, record the volume of the upper and lower phases, and use HPLC to detect the C in the upper and lower phases. 3 G and C 3 R content, the results are shown in Table 1. The results showed that all the experimental groups added immobilized α-L-rhamnosidase C 3 The total recoveries of G are higher than those of free e...

Embodiment 2

[0035] Take fresh mulberries, squeeze the juice, filter and centrifuge to obtain mulberry juice. Weigh 35wt.% ethanol, 15wt.% ammonium sulfate, 5wt.% mulberry juice and the rest of water and mix well to form a two-phase aqueous system, and react at pH 3.5 and temperature 20°C for 0.5h. After the reaction, centrifuge and stand still, take samples from the upper and lower phases respectively, record the volume of the upper and lower phases, and use HPLC to detect the C in the upper and lower phases. 3 G and C 3 R content. Calculated, C 3 The recovery of G was 53.91 ± 0.74%, C 3 The recovery of R was 52.74 ± 0.64%.

[0036] Take the same system as above, and after it is stabilized, add 2wt.% immobilized α-L-rhamnosidase (enzyme activity 15.73±0.03U / mg) to form a liquid-liquid-solid three-phase system at pH 3.5 and temperature 30°C Under the condition of reaction 1h. After the reaction, centrifuge and stand still, take samples from the upper and lower phases respectively, re...

Embodiment 3

[0038] Take fresh mulberries, squeeze the juice, filter and centrifuge to obtain mulberry juice. Weigh 27.12wt.% of ethanol, 18.10wt.% of ammonium sulfate, 12.93wt.% of mulberry juice and the rest of the water and mix thoroughly to form a two-phase system, and react at pH 4.5 and temperature 35°C for 0.5h . After the reaction, centrifuge and stand still, take samples from the upper and lower phases respectively, record the volume of the upper and lower phases, and use HPLC to detect the C in the upper and lower phases. 3 G and C 3 R content. Calculated, C 3 The recovery of G was 73.71 ± 1.02%, and C 3 The recovery of R was 68.37±1.13%.

[0039] Take the same system as above, after it is stabilized, add 4.24wt.% immobilized α-L-rhamnosidase (enzyme activity 15.73±0.03U / mg) to form a liquid-liquid-solid three-phase system, at pH 5, temperature 45 Under the condition of ℃, react for 1h. After the reaction, centrifuge and stand still, take samples from the upper and lower p...

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Abstract

The invention discloses a method for extracting and separating mulberry red pigments by liquid-liquid-solid system enzymatic hydrolysis. A three-phase system comprises, by total weight, 15-30wt.% of ethyl alcohol, 15-25wt.% of ammonium sulfate, 5-20wt.% of mulberry juice, 2-10wt.% of immobilized alpha-L-rhamnosidase (with enzyme activity of 15.73+ / -0.03U / mg) and balance water. Under the conditionsof the pH (potential of hydrogen) of 3.5-5.5, the temperature of 20-50 DEG C and the reaction time of 1-3 hours, the mulberry red pigments are extracted and separated. Cornflower-3-O-rutinoside (C3R)in the mulberry red pigments is catalyzed by the immobilized alpha-L-rhamnosidase, rhamnoside bonds are specifically fractured and converted into cornflower-3-O-glucoside (C3G) in an oriented manner,and high-concentration C3G is prepared. The generation process is environmentally friendly, pollution is avoided, enzyme can be reused, the method is low in cost and high in recovery rate, enrichmentof a single component is realized, and products are good in water solubility and pigmenting property and have quite wide market prospects.

Description

technical field [0001] The invention belongs to the field of biochemical industry, and in particular relates to a liquid-liquid-solid system enzyme-catalyzed hydrolysis-extraction method for separating mulberry red pigment Background technique [0002] Mulberry (Mulberry fruit) has another name called mulberry fruit, is the fruit of the deciduous tree mulberry (Morus alba L.) of the family Moraceae, and matures in April-June every year. In 1993, the National Ministry of Health listed mulberry as one of the agricultural products of "both food and medicine". Edible mulberry fruit can enhance the weight of immune organs, enhance non-specific immune function and humoral immune function, prevent cell mutation, and also have anti-virus effects such as hepatitis B and AIDS (Journal of Nutritional Biochemistry, 2015, 26 (8): 860-867). Mulberry is rich in red pigment, which is one of the 48 kinds of natural pigments officially approved by my country. Mulberry red pigment is an ant...

Claims

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Application Information

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IPC IPC(8): C12P19/60C12P19/14
CPCC12P19/14C12P19/60
Inventor 王俊周雪皎朱长通张露月盛晟吴福安
Owner JIANGSU UNIV OF SCI & TECH
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