Serum and plasma drug extraction composition and application thereof
A composition and extraction technology, applied in the field of medicine, can solve the problems affecting the response value and the efficiency of drug detection, etc.
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Embodiment 1
[0022] 1. Materials
[0023] 1.1 Equipment: 10mL measuring flask, volumetric flask, vortex instrument, centrifuge, liquid chromatography system, triple quadrupole mass spectrometer.
[0024] 1.2 Reagents; pharmaceutical standards, extraction compositions, internal standard compounds, blank serum, and serum for detection are taken from real patient data.
[0025] 2. Test sample preparation steps:
[0026] Preparation of standard solution: Accurately weigh 10 mg of each drug standard, place it in a 10 mL volumetric bottle, dissolve the plasma drug standard with the extraction composition, constant volume, and dilute to make a standard with a concentration of 1.0 mg / mL solution, wherein the pharmaceutical standard includes mirtazapine, O-desvenlafaxine, hydroxybupropion, bupropion, 9-hydroxy-risperidone, risperidone, trazodone, ammonia Sulpride, clonazepam, alprazolam, sertraline, and duloxetine correspond to: MDP, O-QJWLFX, QAFTT, AFTT, 9-OH-LPT, LPT, QZT, AHBL, LXXP, APZL, S...
Embodiment 2
[0036] 1. Materials
[0037] 1.1 Equipment: 10mL measuring flask, volumetric flask, vortex instrument, centrifuge, liquid chromatography system, triple quadrupole mass spectrometer.
[0038] 1.2 Reagents; pharmaceutical standards, extraction compositions, internal standard compounds, blank plasma, and plasma for testing are taken from real patient data.
[0039] 2. Test sample preparation steps:
[0040] Preparation of the internal standard solution: dissolve and transfer the internal standard compound (here, the internal standard corresponding to the pharmaceutical standard) to the measuring bottle with the extraction composition to obtain an internal standard solution with a concentration of 100 μg / mL, and obtain the concentration after dilution 25.0ng / mL internal standard working solution;
[0041] Preparation of serum test samples: Precisely draw 100 μL of plasma sample, add 400 μL of internal standard solution, vortex mix for 3 minutes, centrifuge at 11,000 rpm at 4°C for...
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Description
Claims
Application Information
- IPC
- G01N30/02; G01N30/06
- CPC
- G01N30/02; G01N30/06; G01N2030/062
- Inventors
- 彭军; 吴超超