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Serum and plasma drug extraction composition and application thereof

A composition and extraction technology, applied in the field of medicine, can solve the problems affecting the response value and the efficiency of drug detection, etc.

Inactive Publication Date: 2018-12-25
HANGZHOU BAICHEN MEDICAL INSTR CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after the patient takes the drug, the drug enters the blood in the form of the original drug or metabolite after absorption, and then binds to the protein in the plasma, and then transports in the body to the site of action of the drug in the form of protein binding, so the liquid chromatography tandem Before mass spectrometry detection, it is necessary to extract the drug and / or drug metabolites in the test sample. The extraction efficiency will greatly affect the response value of the detection instrument to the detection target, that is, it will greatly affect the drug detection. efficiency

Method used

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  • Serum and plasma drug extraction composition and application thereof
  • Serum and plasma drug extraction composition and application thereof
  • Serum and plasma drug extraction composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Materials

[0023] 1.1 Equipment: 10mL measuring flask, volumetric flask, vortex instrument, centrifuge, liquid chromatography system, triple quadrupole mass spectrometer.

[0024] 1.2 Reagents; pharmaceutical standards, extraction compositions, internal standard compounds, blank serum, and serum for detection are taken from real patient data.

[0025] 2. Test sample preparation steps:

[0026] Preparation of standard solution: Accurately weigh 10 mg of each drug standard, place it in a 10 mL volumetric bottle, dissolve the plasma drug standard with the extraction composition, constant volume, and dilute to make a standard with a concentration of 1.0 mg / mL solution, wherein the pharmaceutical standard includes mirtazapine, O-desvenlafaxine, hydroxybupropion, bupropion, 9-hydroxy-risperidone, risperidone, trazodone, ammonia Sulpride, clonazepam, alprazolam, sertraline, and duloxetine correspond to: MDP, O-QJWLFX, QAFTT, AFTT, 9-OH-LPT, LPT, QZT, AHBL, LXXP, APZL, S...

Embodiment 2

[0036] 1. Materials

[0037] 1.1 Equipment: 10mL measuring flask, volumetric flask, vortex instrument, centrifuge, liquid chromatography system, triple quadrupole mass spectrometer.

[0038] 1.2 Reagents; pharmaceutical standards, extraction compositions, internal standard compounds, blank plasma, and plasma for testing are taken from real patient data.

[0039] 2. Test sample preparation steps:

[0040] Preparation of the internal standard solution: dissolve and transfer the internal standard compound (here, the internal standard corresponding to the pharmaceutical standard) to the measuring bottle with the extraction composition to obtain an internal standard solution with a concentration of 100 μg / mL, and obtain the concentration after dilution 25.0ng / mL internal standard working solution;

[0041] Preparation of serum test samples: Precisely draw 100 μL of plasma sample, add 400 μL of internal standard solution, vortex mix for 3 minutes, centrifuge at 11,000 rpm at 4°C for...

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Abstract

The invention provides a serum and plasma drug extraction composition. The extraction composition comprises methanol, acetonitrile, isopropyl alcohol and water, wherein the volume ratio of methanol toacetonitrile to isopropyl alcohol to water is 6:2:1:1. The extraction composition is suitable for the extraction of serum plasma drugs and drug metabolites to obtain released drugs. The released drugs are detected by liquid chromatography tandem mass spectrometry, and the sensitivity and specificity of the detection are improved.

Description

technical field [0001] The present invention relates to the field of medical technology, in particular to a serum plasma drug extraction composition and its application. The serum plasma drug extraction composition is used to release drugs and drug metabolites in human serum / plasma so that these released products can be released in the liquid subsequently. Determination in phase chromatography-tandem mass spectrometry. Background technique [0002] In order to prevent, treat and diagnose diseases, people need to take many drugs in their daily life, including antipsychotic drugs, antidepressant drugs, sedative drugs, antineoplastic drugs, antiviral drugs, etc. However, drugs are Chemical substances that affect the physiological functions of the body’s organs and cell metabolism must be strictly controlled when the drug is used by patients. If the drug concentration is low, the drug effect will not be achieved, and if the drug concentration is high, it will cause side effects ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 彭军吴超超高强
Owner HANGZHOU BAICHEN MEDICAL INSTR CO LTD
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