Preparation method of oligomeric proanthocyanidins

A technology of oligomeric proanthocyanidins and proanthocyanidins, applied in skin care preparations, pharmaceutical formulations, cosmetic preparations, etc., can solve the problems of high hydrogen peroxide concentration, high molecular weight, and increased production costs, and achieve the effect of wide demand and simple operation

Active Publication Date: 2019-01-01
HUNAN DASANXIANG TEA OIL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This patent has the following disadvantages: no reduction polymerization is used, the degree of polymerization of proanthocyanidins is high, the molecular weight is high, it is not easy to be absorbed by the human body and human microorganisms, and the antioxidant capacity is weak
This patent has the following disadvantages: the concentration of hydrogen peroxide is high, and the amount of hydrogen peroxide added is large, it is easy to oxidize the oligomeric proanthocyanidins just produced, and the yield of oligomeric proanthocyanidins is low
A large amount of water is used to extract raw materials at high temperature, which increases production costs, and the yield of oligomeric proanthocyanidins is low, and it is difficult to obtain high-purity proanthocyanidins

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Take 200 grams of crushed grape seeds, add 1000mL of 39% ethanol solution, extract at 50°C for 90 minutes, filter and concentrate the extract to obtain a concentrate, concentrate until the ethanol content in the concentrate is undetectable; put the concentrate on HPD-100 Pore ​​adsorption resin, the column loading flow rate is 3BV / h, the sample loading flow rate is 5BV / h, first wash the column with distilled water, the water washing flow rate is 3BV / h, let the adsorption time stand for 1 hour, use 39% alcohol aqueous solution as the eluent, and the elution flow rate 3BV / h, the amount of eluent is 3BV, and the alcohol eluate is collected. Concentrate the alcohol eluate until it has no alcohol smell, then adjust the proanthocyanidin concentration to 5 mg / mL with water, adjust the pH of the proanthocyanidin solution to 9.0 with sodium carbonate solution, add 19% hydrogen peroxide with 5% of the mass of the proanthocyanidin solution, and polymerize at 60°C for 30 minutes. T...

Embodiment 2

[0035] Take 200 grams of crushed pine bark, add 1400mL30% ethanol solution, extract at 59°C for 60 minutes, filter and concentrate the extract to obtain a concentrated solution, apply the concentrated solution to HPD-100 macroporous adsorption resin, and the flow rate of the upper column is 3BV / h. The sample flow rate is 5BV / h, first wash the column with distilled water, the water washing flow rate is 2BV / h, let the adsorption time stand for 2 hours, use 20% alcohol aqueous solution as the eluent, the elution flow rate is 3BV / h, the eluent dosage is 5BV, collect the alcohol eluent. Concentrate the alcohol eluent until it has no alcohol smell, then adjust the concentration of proanthocyanidins to 8 mg / mL with water, adjust the pH of the proanthocyanidin solution to 8.0 with sodium bicarbonate solution, add 19% hydrogen peroxide with 3% of the mass of the proanthocyanidin solution, and polymerize at 70°C for 20 minutes and spray-drying the depolymerized proanthocyanidin solution...

Embodiment 3

[0037]Take 200 grams of crushed tea seed shells, add 1600mL of 30% ethanol solution, extract at 40°C for 120 minutes, filter and concentrate the extract to obtain a concentrated solution, put the concentrated solution on D101 macroporous adsorption resin, the column flow rate is 3BV / h, and load the sample The flow rate is 5BV / h, first wash the column with distilled water, the flow rate of water washing is 3BV / h, and the adsorption time is 0.5 hours. Use 30% alcohol aqueous solution as the eluent, the elution flow rate is 3BV / h, the amount of eluent is 4.5BV, and the alcohol is collected eluent. Concentrate the alcohol eluent until it has no alcohol smell, then adjust the concentration of proanthocyanidins to 10mg / mL with water, adjust the pH of the proanthocyanidin solution to 8.5 with sodium hydroxide solution, add 19% hydrogen peroxide with 3.5% of the mass of the proanthocyanidin solution, and polymerize at 60°C for 40 minutes and spray-drying the depolymerized proanthocyan...

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Abstract

The invention relates to the technical field of natural plant extraction and processing and specifically relates to a preparation method of oligomeric proanthocyanidins. The preparation method comprises extraction, concentration, purification, re-concentration, water dissolving, aggregation reducing and spray drying to obtain the oligomeric proanthocyanidins. Since the selected raw materials are natural materials, the preparation method provided by the invention can be used for processing food and cosmetics; the selected extraction solvent and dilution solvent are alcohol; moreover, the operation is easy, and the prepared oligomeric proanthocyanidins has quite strong resistance to oxidation.

Description

technical field [0001] The invention relates to the technical field of natural plant extraction and processing, in particular to a preparation method of oligomeric proanthocyanidins. technical background [0002] Proanthocyanidins are polyphenolic compounds of a class of flavanol monomers and their polymers, also known as condensed tannins. Proanthocyanidin is a natural biologically active substance widely distributed in nature. It has the functions of regulating immunity and anti-inflammation, protecting liver and lowering fat, and protecting intestinal damage. ] , prevention and treatment of atherosclerosis, anti-ultraviolet rays, protection of optic nerve, anti-oxidation and other pharmacological activities, it has many applications in the fields of food, health products, pharmaceuticals and cosmetics. [0003] Proanthocyanidins are polymers formed by the polymerization of catechins or / and epicatechins in different quantities. Generally speaking, proanthocyanidins formed...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62A61K8/49A61Q19/08A23L33/105
CPCA61Q19/08A23L33/105A61K8/498C07D311/62A23V2002/00A23V2200/324A23V2200/32A23V2200/30A23V2250/21166A23V2250/21
Inventor 赖琼玮陈劲松杨耀学邓一军凌小辉黄闺
Owner HUNAN DASANXIANG TEA OIL CO LTD
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