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Method for inductively differentiating adipose derived mesenchymal stem cells into vascular endothelial cells

A technology of stem cells and vascular endothelium, applied in the field of stem cells and regenerative medicine, can solve problems such as no obvious therapeutic effect, decreased blood flow, and impact on erectile function, and achieve the effect of promoting family and social stability and improving quality of life

Pending Publication Date: 2019-01-01
GUANGDONG VITALIFE BIOTECHNOLOGY CO LTD
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Problems solved by technology

However, the drug only works on vascular-derived ED, while diabetes-induced ED patients have damage to the vascular sinuses in the penile cavernous body, atherosclerotic plaque formation, and reduced blood flow. Structural or functional damage, affecting erectile function, at this time, traditional PDE5i has no obvious therapeutic effect

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  • Method for inductively differentiating adipose derived mesenchymal stem cells into vascular endothelial cells
  • Method for inductively differentiating adipose derived mesenchymal stem cells into vascular endothelial cells
  • Method for inductively differentiating adipose derived mesenchymal stem cells into vascular endothelial cells

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Embodiment Construction

[0028] The technical solutions of the present invention will be described in further detail below in conjunction with specific examples, but the present invention is not limited to the following examples.

[0029] To induce the differentiation of adipose-derived mesenchymal stem cells into vascular endothelial cells, the steps are as follows:

[0030] 1. 30ml of adipose tissue comes from the abdomen of a healthy adult donor who has undergone liposuction and has signed an informed consent;

[0031] 2. After the above-mentioned adipose tissue is collected in the surgical operating room of the plastic surgery hospital, it is sealed in a transport bottle for refrigerated transportation (4-8°C). 50ml of tissue protection solution needs to be added to the transport bottle, and the final concentration of the tissue protection solution is 25 μg / ml of gentamicin sulfate and 5 μg / ml of amphotericin B to ensure no bacterial and fungal contamination during transportation;

[0032] 3. Af...

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Abstract

The invention provides a method for inductively differentiating adipose derived mesenchymal stem cells into vascular endothelial cells. The method comprises the following steps: washing adipose tissues; adding a tissue digestive enzyme which has an equal volume with the adipose tissues for digestion; after digestion is finished, adding trypsin liquid which has the equal volume with the tissue digestive enzyme for digestion; after the digestion is finished, centrifuging to remove upper-layer oil and digestion liquid, wherein sediment is adipose tissue stromal vascular fraction cells; carrying out re-suspension on the stromal vascular fraction cells again through normal saline and sieving to remove large tissues; washing with the normal saline again and centrifuging to remove supernatant; inoculating and culturing washed stromal vascular cells through a serum-free multiplication culture medium, so as to obtain the adipose derived mesenchymal stem cells; selecting P3-generation adipose derived mesenchymal stem cells and digesting to obtain single-cell suspension; inoculating and culturing overnight by utilizing the serum-free multiplication culture medium; after culturing for 24 h, removing the serum-free multiplication culture medium; and adding an induction culture solution and carrying out induced culture, so as to obtain the differentiated vascular endothelial cells. The method provided by the invention can be used for inducing to obtain the effective vascular endothelial cells.

Description

technical field [0001] The invention belongs to the field of stem cells and regenerative medicine, and in particular relates to a method for inducing adipose-derived mesenchymal stem cells into vascular endothelial cells to treat erectile dysfunction (commonly known as impotence) caused by diabetes. Background technique [0002] Male erectile dysfunction (ED) refers to the continuous inability of the penis to achieve and maintain a sufficient erection to obtain a satisfactory sexual life. It is one of the diseases that threaten the healthy quality of life of men, especially elderly men. The incidence rate is among the elderly and diabetic patients extremely high. [0003] The etiology of ED is complex. In the 1970s, researchers also believed that the onset of ED was related to the decrease of androgen, natural aging and psychological factors. With the deepening of research, people found that ED is related to various metabolic diseases (including diabetes, hypertension and c...

Claims

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Application Information

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IPC IPC(8): C12N5/071C12N5/0775A61P15/10
CPCA61P15/10C12N5/0667C12N5/069C12N2500/38C12N2501/11C12N2501/115C12N2501/165C12N2506/1384C12N2509/00
Inventor 朱建斌江嘉豪何美弟陈炽峰康斯敏陈碧莹蔡祥胜王明珠王进辉
Owner GUANGDONG VITALIFE BIOTECHNOLOGY CO LTD
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