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Antibacterial peptide composite material and preparation method and application thereof

A composite material and antimicrobial peptide technology, applied in the field of biomedicine, to achieve fast and efficient insertion and pore formation, low hemolytic toxicity, and good antibacterial ability

Active Publication Date: 2019-01-04
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, related materials require complex and delicate preparation and purification processes

Method used

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  • Antibacterial peptide composite material and preparation method and application thereof
  • Antibacterial peptide composite material and preparation method and application thereof
  • Antibacterial peptide composite material and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Preparation of Melittin / Graphene Oxide Nanodot Composite Materials by Chemical Modification

[0034] 1. Activate the amino group of melittin

[0035] Melittin was dissolved in PBS buffer to prepare a 2 mg / mL solution. Take 400 μL of this solution (containing 800 μg of melittin), dilute 75 mM TEPA 25 times and add 1 mL into the melittin solution, then add 10 μL of EDC with a concentration of 50 mM, and 10 μL of NHS with a concentration of 100 mM. Rotate and react at 250 rpm for 10 hours at 22°C. Solution A is obtained.

[0036] 2. Preparation of aqueous dispersion of graphene oxide nanodots

[0037] Disperse graphene oxide nanodots (GO NDs, size 39±10nm, about 4-5 layers of graphene oxide single layer) powder in water, prepare 0.5mg / mL aqueous dispersion, and ultrasonically for more than 2 hours to make it uniformly dispersed . Solution B is obtained.

[0038] 3. Chemical modification

[0039] Mix solution A with 200 μL of solution B, and continue to re...

Embodiment 2

[0040] Example 2: Preparation of melittin / graphene oxide nanocrystal composites by chemical modification

[0041] 1. Activate the amino group of melittin

[0042] Melittin was dissolved in PBS buffer to prepare a 2 mg / mL solution. Take 400 μL of this solution (containing 800 μg of melittin), dilute 75 mM TEPA 25 times and add 1 mL into the melittin solution, then add 10 μL of EDC with a concentration of 50 mM, and 10 μL of NHS with a concentration of 100 mM. Rotate and react at 250 rpm for 10 hours at 22°C. Solution A is obtained.

[0043] 2. Preparation of aqueous dispersion of graphene oxide nanocrystals

[0044] Disperse graphene oxide nanocrystal (GO NCs, size 90±10nm, containing about 2-3 layers of graphene oxide monolayer) powder in water to prepare a 0.5mg / mL aqueous dispersion, and ultrasonicate for more than 2 hours to make it uniformly dispersed . Solution B is obtained.

[0045] 3. Chemical modification

[0046]Mix solution A with 200 μL of solution B, and co...

Embodiment 3

[0047] Example 3: Preparation of melittin / graphene oxide nano-dot composite material by physical adsorption method

[0048] 1. Preparation of melittin solution

[0049] Dissolve melittin in PBS buffer to prepare solution A at 2 mg / mL.

[0050] 2. Preparation of aqueous dispersion of graphene oxide nanodots

[0051] Disperse graphene oxide nanodots (GO NDs, size 39±10nm, about 4-5 layers of graphene oxide single layer) powder in water, prepare 0.5mg / mL aqueous dispersion, and ultrasonically for more than 2 hours to make it uniformly dispersed . Solution B is obtained.

[0052] 3. Physical adsorption

[0053] Mix solution A with 200 μL of solution B, and continue to react on a shaker at 250 rpm for 2 h. The reacted liquid is freeze-dried to make an appropriate concentration according to the concentration requirements of melittin / GO.

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Abstract

The invention discloses an antibacterial peptide composite material and a preparation method and application thereof and belongs to the technical field of biomedicine. An antibacterial peptide-graphene composition material is prepared by using natural antibacterial peptide and a small-size graphene material as raw materials and adopting a chemical modification or physical adsorption method. The material has good antibacterial ability and low hemolytic toxicity within an activity concentration range. Especially, antibacterial efficiency of a melittin / graphene oxide composite material prepared by the method is higher than more than 20 times than that of original melittin or graphene oxide. The characteristic that antibacterial peptide and graphene material have similar mechanism of damagingcell membranes is utilized, the antibacterial peptide and the graphene material are synergistic to realize quick and efficient inserting and pore-forming of the cell membranes so as to cause leakage of bacterial content, and effective bacteria killing at extremely low drug concentration is realized.

Description

technical field [0001] The invention relates to an antibacterial peptide composite material and its preparation method and application, belonging to the technical field of biomedicine. Background technique [0002] Bacterial infection has always been a fatal problem threatening human health. Since the discovery of penicillin in 1928, people have mainly used antibiotics to suppress bacteria. However, the overuse of antibiotics has led to the emergence of bacterial resistance. In recent years, bacterial drug resistance has become one of the most serious problems threatening human public health. The World Health Organization estimates that about 700,000 people worldwide die each year due to bacterial resistance, and the situation will become even worse in the future. Therefore, it is imminent to adopt a new idea to develop new antibacterial drugs. [0003] Antimicrobial peptides are expected to fundamentally solve the problem of bacterial resistance. As the first line of d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/16A61K47/52A61P31/04
CPCA61K38/16A61K47/52A61P31/04Y02A50/30
Inventor 元冰杨恺卢雪梅刘娇娇
Owner SUZHOU UNIV
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