Efficient synthesis of solvents and organic acids via butanol fermentation

A technology of butanol fermentation and organic acid, which is applied in the field of microbial fermentation, can solve the problems of low butyric acid concentration, low butyric acid/butanol, inability to obtain high value-added products of in-situ synthesis of butyl butyrate, and achieve high-efficiency synthesis , increase production and reduce the cost of fermentation raw materials

Active Publication Date: 2020-02-14
HUAIYIN INSTITUTE OF TECHNOLOGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the concentration of butyric acid is low at the end of fermentation, and the butyric acid / butanol ratio is low, so it is impossible to obtain in-situ synthesis of butyl butyrate or other high value-added products

Method used

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  • Efficient synthesis of solvents and organic acids via butanol fermentation
  • Efficient synthesis of solvents and organic acids via butanol fermentation
  • Efficient synthesis of solvents and organic acids via butanol fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 2

[0039] Embodiment 2: Butanol fermentation process with 0.2 g / L vanillin added

[0040] The preparation process of the activation solution is exactly the same as that in Embodiment 1, and will not be repeated here.

[0041] The preparation process of the Clostridium acetobutylicum seed solution is exactly the same as that in Embodiment 1, and will not be repeated here.

[0042] The Clostridium acetobutylicum seed solution was transferred to a 5 L anaerobic fermentation tank with an inoculum of 10% (v / v), and the anaerobic fermentation tank was filled with 3 L Clostridium fermentation medium (where the glucose concentration was 60 g / L, the vanillin concentration is 0.2 g / L), the fermentation temperature is 37°C, and the pH control strategy during the fermentation process is expressed as follows: During the acid production period (~0-15 h), the pH is automatically controlled to 5.0 by the control cabinet , using 14% (w / v) ammonia water; during the solventogenic phase (~15-48 h)...

Embodiment approach 3

[0046] Embodiment 3: Butanol fermentation process with 0.2 g / L vanillic acid added

[0047] The preparation process of the activation solution is exactly the same as that in Embodiment 1, and will not be repeated here.

[0048] The preparation process of the Clostridium acetobutylicum seed solution is exactly the same as that in Embodiment 1, and will not be repeated here.

[0049] The Clostridium acetobutylicum seed solution was transferred to a 5 L anaerobic fermentation tank with an inoculum of 10% (v / v), and the anaerobic fermentation tank was filled with 3 L Clostridium fermentation medium (where the glucose concentration was 60 g / L, the concentration of vanillic acid is 0.2 g / L), the fermentation temperature is 37°C, and the pH control strategy during the fermentation process is expressed as follows: During the acid production period (~0-15 h), the pH is automatically controlled to 5.0 by the control cabinet, 14% (w / v) ammonia was utilized; during the solventogenic pha...

Embodiment approach 4

[0053] Embodiment 4: Butanol fermentation process with addition of 0.1 g / L vanillin and 0.1 g / L vanillic acid

[0054] The preparation process of the activation solution is exactly the same as that in Embodiment 1, and will not be repeated here.

[0055] The preparation process of the Clostridium acetobutylicum seed solution is exactly the same as that in Embodiment 1, and will not be repeated here.

[0056]The Clostridium acetobutylicum seed solution was transferred to a 5 L anaerobic fermentation tank with an inoculum of 10% (v / v), and the anaerobic fermentation tank was filled with 3 L Clostridium fermentation medium (where the glucose concentration was 60 g / L, the concentration of vanillin is 0.1 g / L, the concentration of vanillic acid is 0.1 g / L), the fermentation temperature is 37°C, and the pH control strategy during the fermentation process is expressed as follows: During the acid production period (~0-15 h) , the pH is automatically controlled to 5.0 through the con...

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Abstract

The present invention involves the field of microbial fermentation, and a method of high -efficiency synthetic solvents and organic acids through butanol fermentation is disclosed, including the following steps: S1: Patorateraxol in the spore tubeRepeat it, then transfer to the cultivation of bacteria growth culture medium to obtain high -vibrant acetorobitanol spinthromes; S2: Transfer the acetorob -orblobacrerobacterium melterol to the measonal liquidAlcohol, acetone, ethanol, ethanol, ethidic acid, and butyl acid; the first stage of fermentation is the acid production period, the pH of the fermentation liquid of the acid production period is controlled at 5.0; the fermentation is performed until the solvent period, and the pH is not controlled; S3: fermentation in bacteria fermentationThe phenolic substance is added to the medium, and the dynamic characteristics of the cytanol fermentation process are examined in the 5 L anaerobic fermentation tank.The present invention simply adds a small amount of fermentation inhibitory -phenols to achieve the goals of solvents and organic acids at the same time, and ultimately improve the fermentation performance of butanol.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a method for efficiently synthesizing solvents and organic acids through butanol fermentation. Background technique [0002] Acetone-butanol-ethanol fermentation, also known as ABE (Acetone-Butanol-Ethanol) fermentation or butanol fermentation, is the use of Clostridium difficile ( Clostridia ) fermentation process for solvent production of acetone, butanol and ethanol under strictly anaerobic conditions. Butanol, the main product of fermentation, is an important platform compound, and at the same time, it is also a new type of clean and renewable liquid fuel and diesel combustion improver with great potential. For a long time, people prefer to use ABE fermentation to obtain high-purity butanol solvent. However, the lipophilicity of butanol will destroy the phospholipid structure of the cell membrane, enhance the fluidity of the cell membrane, and greatly damage the cells....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/06C12P7/16C12P7/28C12P7/52C12P7/54C12R1/145
CPCC12P7/065C12P7/16C12P7/28C12P7/52C12P7/54Y02E50/10
Inventor 罗洪镇郑盼丽谢方杨荣玲刘帅曾庆伟
Owner HUAIYIN INSTITUTE OF TECHNOLOGY
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