A medicine for treating bone marrow hematopoietic system injury and repairing

A technology of medicine and bone marrow transplantation, applied in the field of medicine

Inactive Publication Date: 2019-01-11
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the effect of ABC294640 on hematopoietic stem cells has not been reported

Method used

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  • A medicine for treating bone marrow hematopoietic system injury and repairing
  • A medicine for treating bone marrow hematopoietic system injury and repairing
  • A medicine for treating bone marrow hematopoietic system injury and repairing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] After bone marrow transplantation of hematopoietic stem cells, injection of SphK2 gene inhibitor ABC294640 significantly increased the number of hematopoietic stem cells.

[0017] Twenty-five 8-week-old C57 mice (purchased from the Experimental Animal Center of Sun Yat-Sen University) were selected as experimental mice and raised in a specific pathogen-free (SPF) environment. Take 5 of the C57 mice, kill them by neck dislocation, take their femurs and tibias, use a 1ml syringe to flush out the bone marrow in the bone marrow cavity, blow it into a single cell suspension, and lyse the red blood cell lysate for 2 minutes to remove the red blood cells, filter them with 70um Filter through a mesh to obtain a bone marrow single cell suspension. Another 20 C57 mice were subjected to X-ray radiation injury with a dose of 900cGy, and then each irradiated mouse was injected with 400,000 bone marrow single cell suspensions through the tail vein. Next, the mice that had undergone ...

Embodiment 2

[0021] Inhibitor ABC294640 injection significantly accelerated platelet recovery after hematopoietic stem cell bone marrow transplantation.

[0022] The preparation of the SphK2 inhibitor was consistent with that in Example 1. Twenty 8-week-old C57 mice purchased from the Animal Center of Sun Yat-sen University were selected and divided into two groups, each with 10 mice. Same as in Example 1, the injection of the inhibitor ABC294640 and the solvent control was the same as in Example 1. The injection was also started on the second day after irradiation, and then every two days at a dose of 10 mg / kg until the end of the experiment. In the first week, the second week, the third week, the fourth week and the fifth week (the 7th, 14th, 21st, 28th, and 35th days), blood was taken from the tail vein of the mice in an anticoagulant tube, and blood routine detection was used Peripheral blood platelet count. We used Graphpad6.0 for t-test analysis, and marked the significance of the d...

Embodiment 3

[0025] Inhibitor ABC294640 injection significantly accelerated the recovery of white blood cells after hematopoietic stem cell bone marrow transplantation.

[0026] The preparation of the SphK2 inhibitor was consistent with that in Example 1. Twenty 8-week-old C57 mice purchased from the Animal Center of Sun Yat-sen University were selected and divided into two groups, each with 10 mice. Same as in Example 1, the injection of the inhibitor ABC294640 and the solvent control was the same as in Example 1. The injection was also started on the second day after irradiation, and then every two days at a dose of 10 mg / kg until the end of the experiment. In the first week, the second week, the third week, the fourth week and the fifth week (the 7th, 14th, 21st, 28th, and 35th days), blood was taken from the tail vein of the mice in an anticoagulant tube, and blood routine detection was used Peripheral blood leukocyte count. We used Graphpad6.0 for t-test analysis, and marked the sign...

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Abstract

The present invention provides the use of SphK2 inhibitor ABC294640 in the preparation of a drug for promoting hematopoietic and immune system reconstruction after bone marrow transplantation. ABC294640 can increase the number and function of hematopoietic stem, and enhance its ability to differentiate into many blood cells and immune cells, which can help patients quickly through the critical period after transplantation. The invention provides a novel drug source for treating complications of various hematopoietic dysfunction of bone marrow transplantation patients.

Description

technical field [0001] The invention belongs to the field of medicine, and specifically relates to the application of ABC294640 in the treatment of hematopoietic and immune system function recovery disorders in bone marrow transplantation patients. Background technique [0002] Hematopoietic stem cells are a group of primitive hematopoietic cells that exist in hematopoietic tissues. Their main function is to differentiate and produce various blood immune cells such as red blood cells, platelets, and white blood cells, and support the renewal of the body's hematopoiesis and immune system. Hematopoietic stem cells play an important role in maintaining normal hematopoietic function and repairing damage. Primary lesions, radiation in the environment, chemical drugs in clinical treatment, and radiation all have serious damage to hematopoietic stem cells, leading to hematopoietic dysfunction, decreased blood count, decreased white blood cells, immune dysfunction, and even reduced ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4409A61P7/00A61P35/00A61P37/02
CPCA61K31/4409A61P7/00A61P35/00A61P37/02
Inventor 赵萌
Owner SUN YAT SEN UNIV
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