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Carbon-fixing rhodococcus and application thereof

A rhodococcus and carbon source technology, applied in the field of rhodococcus, can solve the problems of unknown microbial functions

Active Publication Date: 2019-01-11
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Previous studies have mostly focused on plant carbon sequestration, but whether microorganisms in the soil have the function of fixing atmospheric carbon dioxide has not been known

Method used

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  • Carbon-fixing rhodococcus and application thereof
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  • Carbon-fixing rhodococcus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Isolation and identification of bacterial strain 201806YC01

[0024] Taking the soil at the southwestern edge of the Mu Us Sandy Land in Inner Mongolia as the target soil, the specific screening scheme is as follows:

[0025] 1. Collect the soil at a depth of 0-20cm, take 1.0g of the collected soil, add it to 9mL sterilized water, and shake it for 10min to make a soil solution. Then, take another 1 mL of soil suspension and put it in 9 mL of sterilized water to dilute the soil solution. Repeat this operation. According to previous research experience, the soil solution was diluted to 10 -3 times. Take 100 μL of the soil dilution, inoculate it into inorganic salt MSM medium, and culture it at 25°C for 7 days. The composition of MSM medium is: Na 2 HPO 4 2H 2 O, 7.8g / L; KH 2 PO 4 , 6.8g / L; Na 2 S·9H 2 O, 0.187g / L; MgSO 4 , 0.2g / L; Ca(NO 3 ) 2 4H 2 O, 0.05g / L; NH 4 NO 3 , 0.085g / L; FeSO 4 ·7H 2 O, 0.4g / L; trace element solution, 1mL / L; NaHCO 3...

Embodiment 2

[0028] Example 2 The process of producing carbonate by bacterial strain 201806YC01

[0029] The process of producing carbon-containing minerals by Rhodococcus sp.201806YC01 strain was tested by using organic carbon source medium. Firstly, 100 mL of liquid medium was prepared and placed in a 250 mL Erlenmeyer flask, and sterilized by moist heat method, that is, sterilized at 121° C. for 15 minutes. The composition of the liquid medium is: yeast extract 5g / L, peptone 10g / L, sodium chloride 5g / L, calcium chloride 2g / L. Then, the bacterial strain was inoculated into a liquid culture medium with an inoculation needle, placed at 25° C., and cultivated under the condition of avoiding light. It was observed every 3 days whether there was precipitation, and the fifth observation found that there was precipitation. In order to obtain sufficient precipitation for subsequent analysis, the process was co-cultivated for 30 days. After the cultivation, the generated precipitates were colle...

Embodiment 3

[0033] Example 3 Verification experiment of bacterial strain 201806YC01 precipitating atmospheric carbon dioxide

[0034] The operation method of Example 2 was adopted to prepare culture medium and inoculate bacterial strains. A total of 6 culture flasks were set up for the experiment, and 3 were placed in 13 CO 2 cultured in the environment to label atmospheric CO 2 the whereabouts of; three put in nothing 13 CO 2 cultured in a labeled environment as a control. After the cultivation, the precipitate was treated with hydrogen peroxide solution, the organic matter was oxidized and removed, and then the inorganic precipitate was measured. 13 C abundance value.

[0035] The result is as Figure 4 shown in 13 CO 2 carbonaceous minerals 13The C abundance value was 953.00‰, which was significantly higher than -11.52‰ in the control group, indicating that part of the C in the carbon-bearing minerals in the marked group was derived from atmospheric CO 2 . showed that the s...

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Abstract

The invention discloses carbon-fixing rhodococcus and an application thereof, and in particular relates to a (Rhodococcus sp.) 201806YC01 having the ability to induce the formation of carbon-containing minerals. The invention also relates to a process for biosynthesis of carbon-containing minerals by the Rhodococcus sp. The invention discloses that the Rhodococcus has the ability of fixing atmospheric carbon dioxide for the first time, and has important scientific significance for slowing down the atmospheric greenhouse effect and alleviating global warming.

Description

technical field [0001] The invention belongs to the technical field of microbial carbon fixation, and in particular relates to rhodococcus with carbon fixation ability and application thereof. Background technique [0002] Atmospheric carbon dioxide can be fixed by plants and stored in soil as organic and inorganic carbon. Previous studies have mostly focused on plant carbon sequestration, but whether microorganisms in the soil have the function of fixing atmospheric carbon dioxide has not been known. In desert ecosystems, due to the sparse vegetation and low productivity, it is of great significance to study the ability of microorganisms to fix atmospheric carbon dioxide for understanding the process of carbon accumulation in desert ecosystems. To this end, it is necessary to explore the potential of microorganisms in desert soils to generate carbon-containing minerals and the ability to transform atmospheric carbon dioxide. Contents of the invention [0003] The object...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P3/00C12R1/01
CPCC12N1/20C12P3/00C12N1/205C12R2001/01
Inventor 张宇清刘振秦树高毛赫楠苗琳孙延菲闫茹王晶
Owner BEIJING FORESTRY UNIVERSITY
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