Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Spindle-shaped lysine bacillus with degradation ability of ethanethiol and dimethyl disulfide and application thereof

A technology of Bacillus lysine and dimethyl disulfide, applied in the field of microorganisms, can solve the problem of not seeing the simultaneous degradation of Bacillus lysine spindle, and achieve the effect of efficient degradation

Active Publication Date: 2019-01-18
GUANGDONG UNIV OF TECH
View PDF5 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been no relevant reports and patents on the simultaneous degradation of ethanethiol and dimethyl disulfide using Spinosa lysinus.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Spindle-shaped lysine bacillus with degradation ability of ethanethiol and dimethyl disulfide and application thereof
  • Spindle-shaped lysine bacillus with degradation ability of ethanethiol and dimethyl disulfide and application thereof
  • Spindle-shaped lysine bacillus with degradation ability of ethanethiol and dimethyl disulfide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A strain of Lysinibacillus fusiformis GDUTAN6 with the ability to degrade ethanethiol and dimethyl disulfide at the same time, the strain is preserved in the China Center for Type Culture Collection , the deposit address is Wuhan University Campus, No. 299 Bayi Road, Wuchang District, Wuhan City, Hubei Province; the deposit number is CCTCC NO: M 2018416, and the deposit date is July 2, 2018.

[0027] The spindle-shaped lysine bacillus GDUTAN6 of the present embodiment was isolated and screened from a landfill leachate in Guangzhou City, Guangdong Province, and its separation and purification method was as follows:

[0028] The acclimatization medium used is inorganic salt medium (every 1000mL inorganic salt medium contains K 2 HPO 4 ·3H 2 O1.2g, KH 2 PO 4 1.2g, NH 4 Cl 0.4g, MgSO 4 ·7H 2 O 0.2g, FeSO 4 ·7H 2 O 0.01g, 1mL trace element solution (g / L): CaCl 2 2H 2 O 0.2g, MnSO 4 4H 2 O 0.2g, CuSO 4 2H 2 O 0.01g, ZnSO 4 ·7H 2 O 0.2g, CoCl 2 ·6H 2 O 0.09g...

Embodiment 2

[0053] This example is the application of Lysinibacillus fusiformis GDUTAN6 in environmental restoration, which can degrade ethanethiol in the environment. The environment includes the atmosphere, water body or soil.

[0054] The method that the ethanethiol degradation ability of the spindle-shaped lysine bacillus (Lysinibacillus fusiformis) GDUTAN6 obtained by the screening of the present invention is verified as follows below:

[0055] According to the needs of the degradation experiment, the inorganic salt medium needs to be configured, and 45 mL of the inorganic salt solution is added to the 250 mL serum bottle (every 45 mL of the inorganic salt solution contains: K 2 HPO 4 ·3H 2 O 0.054g, KH 2 PO 4 0.054g, NH 4 Cl 0.018g, MgSO 4 ·7H 2 O0.009g, FeSO 4 ·7H 2 O 0.00045g, CaCl 2 2H 2 O 0.009g, MnSO 4 4H 2 O 0.009g, CuSO 4 2H 2 O0.00045g, ZnSO 4 ·7H 2 O 0.009g, CoCl 2 ·6H 2 O 0.00405g, Na 2 MoO 4 2H 2 O 0.054g, H 3 BO 3 0.00027g, double distilled water ...

Embodiment 3

[0061] This example is the application of Lysinibacillus fusiformis GDUTAN6 in environmental remediation, which can also degrade dimethyl disulfide in the environment. The environment includes the atmosphere, water body or soil.

[0062] The method that the dimethyl disulfide degrading ability of the spindle-shaped lysine bacillus (Lysinibacillus fusiformis) GDUTAN6 obtained by the screening of the present invention is verified as follows below:

[0063] According to the needs of the degradation experiment, the inorganic salt medium needs to be configured, and 45 mL of the inorganic salt solution is added to the 250 mL serum bottle (every 45 mL of the inorganic salt solution contains: K 2 HPO 4 ·3H 2 O 0.054g, KH 2 PO 4 0.054g, NH 4 Cl 0.018g, MgSO 4 ·7H 2 O0.009g, FeSO 4 ·7H 2 O 0.00045g, CaCl 2 2H 2 O 0.009g, MnSO 4 4H 2 O 0.009g, CuSO 4 2H 2 O0.00045g, ZnSO 4 ·7H 2 O 0.009g, CoCl 2 ·6H 2 O 0.00405g, Na 2 MoO 4 2H 2 O 0.054g, H 3 BO 3 0.00027g, double...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a spindle-shaped lysine bacillus with the degradation ability of ethanethiol and dimethyl disulfide and the application thereof. The strain was named Lysinibacillus fusiformisGDUTAN6, Deposited at China Typical Culture Collection, Wuhan University, Wuhan City, Hubei Province, CCTCC NO: M 2018416, dated 2 July 2018. GDUTAN6 is gram-positive, rod-shaped, round, white, transparent, and 1-2mm in diameter, and that bacterial colony of the spindle-shaped lysine bacillus GDUTAN6 is round and transparent. The degradation rate of ethanethiol and dimethyl disulfide was 93.17% and 100%, respectively. The biodegradation of ethanethiol and dimethyl disulfide by Bacillus spindle GDUTAN6 could be used in environmental remediation.

Description

technical field [0001] The invention belongs to the technical field of microbes, and more specifically relates to a spindle-shaped lysine bacillus strain capable of degrading ethanethiol and dimethyl disulfide and application thereof. Background technique [0002] As we all know, organic odor pollution is a very serious air pollution that exists all over the world. It has caused great troubles to people's lives and seriously affected people's physical and mental health. Among them, sulfur-containing odor pollutants are a very important type of odor pollutants. Odor pollution is mainly a kind of olfactory pollution that is perceived by the human olfactory organs through the spread and diffusion of the atmosphere due to the volatility of this kind of odor pollutants. This type of pollutants usually includes volatile inorganic compounds (Volatile Inorganic Compounds, VIC) and volatile organic compounds (Volatile Organic Compounds, VOCs). Wherein ethanethiol (ethanethiol, CH ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20B01D53/84B01D53/72C02F3/34B09C1/10C12R1/01C02F101/34
CPCB01D53/72B01D53/84B09C1/10C02F3/34C02F2101/34C02F2101/40C12N1/205C12R2001/01Y02A50/20
Inventor 李桂英王集军安太成熊举坤
Owner GUANGDONG UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com