A production method of a compound bacterial preparation and an application thereof in sewage treatment

A production method and composite bacteria technology, applied in biological water/sewage treatment, water/sludge/sewage treatment, microbial-based methods, etc., can solve problems such as long domestication cycle, lack of ammonia nitrogen, and weak shock load resistance. It achieves the effect of low requirements for storage conditions, large effective biomass and easy operation

Inactive Publication Date: 2019-01-18
宜兴市永洁环保设备有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] A large amount of chloride ions contained in high-salt wastewater can inhibit the growth of microorganisms and dehydrate microbial cells; reduce the activity of dehydrogenase and cause toxicity to cells; at the same time, due to the increase in water density, activated sludge is easy to float and lose
These adversely affect the traditional biochemical treatment of high-salt wastewater
Ordinary activated sludge can be used to treat high-salt wastewater after a certain period of salt-tolerant acclimation, but the acclimatization period is long, the shock load resistance is weak, and the treatment effect cannot meet actual needs
[0004] At present, most of the salt-tolerant microorganisms screened are functional strains for COD degradation, and there is a lack of salt-tolerant nitrifying bacteria and salt-tolerant denitrifying bacteria for the removal of ammonia nitrogen, especially nitrate nitrogen.

Method used

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  • A production method of a compound bacterial preparation and an application thereof in sewage treatment
  • A production method of a compound bacterial preparation and an application thereof in sewage treatment

Examples

Experimental program
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Effect test

Embodiment 1

[0009] Example 1: Obtaining Salt Tolerance Genes

[0010] The strain of Haloferax mediterranei was purchased from China General Microorganism Culture Collection and Management Center, with the preservation number CGMCC1.3716. Previous studies have proved that the bacteria has strong salt tolerance. Although it has a strong desalination effect, it is not easy to cultivate and does not have the functions of carbon removal, sulfur removal, ammonia nitrogen removal, etc., and cannot be directly applied to the treatment of high-salt wastewater. middle.

[0011] In order to excavate the salt-tolerance-related genes of Mediterranean halobacteria, explore the molecular mechanism of salt-tolerance, and extract its genomic DNA for whole-genome sequencing, the genome sequencing was commissioned by Nanjing GenScript Company through the SOLEXA sequencing platform, using the Escherichia coli expression system (E. .coli DH5a) to construct the cDNA library of Mediterranean halobacteria after...

Embodiment 2

[0013] Example 2: Transferring Salt Tolerance Genes into Marine Facultative Bacillus

[0014] The nucleotide sequences of AQE1, AQE2, AQE3, AQE4, AQE5, AQE6, AQE7, AQE8, AQE9, and AQE10 were synthesized by Nanjing GenScript Biotechnology Co., Ltd., and the 5' end of the synthesized sequence was also connected with an NcoI restriction site point, the 3' end is also connected with a SwaI restriction site. The synthetic AQE1, AQE2, AQE3, AQE4, AQE5, AQE6, AQE7, AQE8, AQE9, AQE10 nucleotide sequences were respectively connected into the cloning vector pGEM-T (Promega, Madison, USA, CAT: A3600), and the operation steps were as follows: Promega company's product pGEM-T vector instructions, obtained recombinant cloning vectors pGEM-T01, pGEM-T02, pGEM-T03, pGEM-T04, pGEM-T05, pGEM-T06, pGEM-T07, pGEM-T08, pGEM-T09, pGEM-T10, the vector structure includes: Amp means the ampicillin resistance gene; f1 means the replication origin of phage f1; LacZ is the LacZ start codon; SP6 is the S...

Embodiment 3

[0023]Prepare a new type of hydrogel carrier composite bacteria preparation system, specifically, according to the number of parts by mass, first dissolve 50 parts of sodium hydroxide in water, and add 50 parts of acrylic acid for pretreatment; then add 20 parts of sodium polyacrylate, corn 10 parts of starch, 10 parts of acrylamide and 2.5 parts of calcium carbonate, after stirring and heating reaction, add initiator to carry out graft polymerization reaction; then put the primary liquid obtained from the reaction into a sterilizer to sterilize to room temperature, and then add to the primary Add composite bacteria preparation suspension and photoinitiator into the liquid, stir evenly, pour into advection vessel, irradiate with weak ultraviolet rays for 10 minutes to make it gel, and obtain a new type of hydrogel carrier composite bacteria preparation system, the composite bacteria preparation The formula includes 25 parts of genetically modified marine facultative bacillus, 9...

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Abstract

The invention provides a production method of a compound bacterial preparation and an application thereof in sewage treatment. The compound bacterial preparation comprises 25 parts of transgenic marine facultative bacillus, 9 parts of magnetotactic bacteria, 2.5 parts of Halobacterium cordiformis, 3.8 parts of starch salt-solubilizing granular bacteria, 2.5 parts of mycobacterium halophilum, 5 parts of Mycobacterium sporangii, 6.5 parts of salt denitrifying mycobacterium and 2 parts of denitrifying kastraniella. The compound bacterial preparation provided by the invention has strong nitrate nitrogen and ammonia nitrogen removal effect in addition to salt tolerance, and is suitable for the treatment of high-salt wastewater.

Description

technical field [0001] The invention relates to the field of sewage treatment, in particular to a production method of a composite bacteria preparation and its application in sewage treatment. Background technique [0002] The salinity of seawater is about 3%. Industrially, wastewater with a salinity equal to or even higher than that of seawater is called high-salt wastewater. A large amount of high-salt wastewater is discharged in textile printing and dyeing, chemical production process, food processing and pesticide production process. At present, the treatment of high-salt wastewater can be divided into two categories: physical chemical method and biological method. Due to the high content of dissolved organic matter in this type of wastewater, general physical and chemical methods are difficult and costly to deal with, while biological methods are economical and efficient. , Harmless characteristics, is the preferred treatment method. [0003] A large amount of chlorid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C12R1/07
CPCC02F3/34C12N1/20
Inventor 周春松
Owner 宜兴市永洁环保设备有限公司
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