A method for preparing polysaccharides from endophytic fungi of Dendrobium officinale with high immune activity by solid-state fermentation
An endophytic fungus, solid-state fermentation technology, applied in microorganism-based methods, biochemical equipment and methods, fermentation, etc., to achieve the effect of simple and easy process, low equipment requirements, and mild conditions
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Embodiment 1
[0047] (1) The endophytic fungus Fusarium solani DO7 strain of Dendrobium candidum was activated and cultured in PDB medium with pH 6.5 at 28°C for 7 days to obtain the seed liquid of the fermented strain;
[0048] (2) Configure the solid fermentation medium according to the determined composition (80.0wt% rice, 5.0wt% bean dregs, 10.0wt% rice bran, 5.0wt% corn bran, the above percentages are based on lignocellulose), and adjust the moisture content to 55wt% , adjusted pH 6.5 with 1M HCl, dispensed into 1L Erlenmeyer flasks, 200mL per bottle, sterilized at 121°C for 20min after sealing, cooled after taking out, inoculated with 15% (v / v) seed liquid, and fermented at room temperature for 30 days; After the fermentation is complete, collect the mycelium, vacuum dry and grind, then extract in hot water at 90°C for 4 hours, collect the bacteria by filtration, repeat the hot water extraction twice, combine the extracts, concentrate under reduced pressure, precipitate with alcohol at...
Embodiment 2
[0052] (1) The endophytic fungus Fusarium solani DO7 strain of Dendrobium candidum was activated and cultured in PDB medium with pH 6.5 at 25°C for 7 days to obtain the seed liquid of the fermented strain;
[0053] (2) Configure the solid fermentation medium (rice 80.0wt%, bean dregs 5.0wt%, rice bran 10.0wt%, corn bran 5.0wt%) according to the determined composition, adjust the moisture content to 55wt%, adjust the pH to 6.5 with 1M HCl, and pack In a 1L Erlenmeyer flask, 200mL per bottle, sterilized at 121°C for 20min after sealing, cooled after taking out, inoculated with 15% (v / v) seed liquid, and fermented at room temperature for 30 days; after the fermentation was completed, the mycelium was collected, Vacuum drying and grinding, then leaching in hot water at 90°C for 4 hours, collecting the bacteria by filtration, repeating hot water extraction twice, combining the extracts, concentrating under reduced pressure, alcohol precipitation at 4°C for 12 hours, centrifugation a...
Embodiment 3
[0055] (1) The endophytic fungus Fusarium solani DO7 strain of Dendrobium officinale was activated and cultured in PDB medium with pH 6.5 at 28°C for 5 days to obtain the seed liquid of the fermented strain;
[0056] (2) Configure the solid fermentation medium (rice 80.0wt%, bean dregs 5.0wt%, rice bran 10.0wt%, corn bran 5.0wt%) according to the determined composition, adjust the moisture content to 55wt%, adjust the pH to 6.5 with 1M HCl, and pack In a 1L Erlenmeyer flask, 200mL per bottle, sterilized at 121°C for 20min after sealing, cooled after taking out, inoculated with 15% (v / v) seed liquid, and fermented at room temperature for 30 days; after the fermentation was completed, the mycelium was collected, Vacuum drying and grinding, then leaching in hot water at 90°C for 4 hours, collecting the bacteria by filtration, repeating hot water extraction twice, combining the extracts, concentrating under reduced pressure, alcohol precipitation at 4°C for 12 hours, centrifugation...
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