Application of flavone type allelochemicals in spartina alterniflora to algae growth inhibition

A technology for neutralizing and allelochemical substances in Spartina alterniflora, applied in the direction of sterilization/microdynamic water/sewage treatment, etc., can solve the problems of ineffective effect and high cost, and achieve the effect of good ecological security and no secondary pollution

Active Publication Date: 2019-01-25
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current means of controlling Spartina alterniflora are costly and have little effect. The comprehensive utilization of Spartina alterniflora

Method used

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  • Application of flavone type allelochemicals in spartina alterniflora to algae growth inhibition
  • Application of flavone type allelochemicals in spartina alterniflora to algae growth inhibition
  • Application of flavone type allelochemicals in spartina alterniflora to algae growth inhibition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1～4

[0043] Examples 1 to 4 Spartina alterniflora was divided into four main parts: root, stem, leaf and ear respectively, and dried at 65°C to constant weight, and the components of Spartina alterniflora root, stem, leaf and ear were pulverized, and the 60 mesh.

[0044] Extract with 70% ethanol, the ratio of solid to liquid is 1:30 (g:mL). Weigh 2 g each of Spartina alterniflora root, stem, leaf, and panicle component powder, mix with 60 mL of 70% ethanol, bathe in 70°C water for 2 h, centrifuge at 5000 r / min for 10 min, and collect the supernatant; repeat the extraction three times, and combine the supernatant.

[0045] With rutin as the standard substance and 95% ethanol as the solvent, prepare 0.04mg / mL, 0.08mg / mL, 0.12mg / mL, 0.16mg / mL, 0.20mg / mL rutin standard solutions, and use NaNO 2 -Al(NO 3 ) 3 Method color development, scanning rutin standard solution, the maximum absorption wavelength is measured at 501nm, the absorbance of each standard solution is measured at this ...

Embodiment 5

[0049] Firstly, the leaves of Spartina alterniflora were dried at 65° C. to constant weight, crushed, and passed through 60 meshes.

[0050]Weigh 12g of Spartina alterniflora leaf powder, extract with 70% ethanol, and the ratio of solid to liquid is 1:30 (g:mL). Mix 12g of Spartina alterniflora powder with 360mL of 70% ethanol, put it in a water bath at 70°C for 2h, centrifuge at 5000r / min for 10min, collect the supernatant, repeat the extraction three times, and combine the supernatant.

[0051] Secondly, a rotary evaporator was used to distill the extract of Spartina alterniflora leaves under reduced pressure to remove the solvent to obtain an extract with a mass of 3.36 g. The extract was dissolved with 10.5 mL of dimethyl sulfoxide, dissolved in a 35°C water bath and ultrasonically assisted to obtain a 0.32 g / mL allelochemical solution.

[0052] UPLC-MS is used to detect the extracted allelochemicals, and the UPLC spectrum is as follows figure 1 shown.

[0053] UPLC-MS ...

Embodiment 6

[0060] Firstly, the whole plant of Spartina alterniflora was dried at 65°C to constant weight, crushed, and passed through 60 mesh.

[0061] Weigh 12 g of whole plant powder of Spartina alterniflora, extract with 70% ethanol, and the ratio of solid to liquid is 1:30 (g:mL). Mix 12g of Spartina alterniflora whole plant powder with 360mL of 70% ethanol, bathe in 70°C for 2h, centrifuge at 5000r / min for 10min, collect the supernatant, repeat the extraction three times, and combine the supernatant.

[0062] Secondly, the extract solution of the whole plant of Spartina alterniflora was subjected to vacuum distillation to remove the solvent by using a rotary evaporator to obtain an extract with a mass of 2.98 g. The extract was dissolved with 7.45mL dimethyl sulfoxide, dissolved in a 35°C water bath and ultrasonically assisted to obtain a 0.40g / mL allelochemical solution.

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Abstract

The invention discloses application of flavone type allelochemicals in spartina alterniflora to algae growth inhibition. The application comprises the steps that the flavone type allelochemicals in spartina alterniflora are added into an algae containing water body, so that the concentration of the flavone type allelochemicals in the water body is greater than 0.1g/L. The flavone type allelochemicals capable of inhibiting the algae growth are extracted from the spartina alterniflora; the outbreak of harmful algae can be treated and controlled; the resource utilization of the spartina alterniflora can also be realized.

Description

technical field [0001] The invention relates to the field of water body pollution control, in particular to the application of flavonoid allelochemicals in Spartina alterniflora to inhibit the growth of algae. Background technique [0002] With the rapid development of my country's industrial and agricultural production and economy, a large amount of domestic sewage and industrial and agricultural wastewater are discharged into coastal and nearshore waters, resulting in eutrophication of water bodies, causing the outbreak of harmful algae, destroying marine ecosystems, and seriously affecting seafood. The production and marine environment quality directly or indirectly endanger human health. How to control harmful algal blooms has become a research hotspot in the field of environment. [0003] At present, the methods of controlling red tide mainly include physical methods, chemical methods and biological methods. Physical methods mainly include mechanical or manual salvage,...

Claims

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Application Information

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IPC IPC(8): C02F1/50
CPCC02F1/50
Inventor 徐彩彩肖溪葛志伟万方浩
Owner ZHEJIANG UNIV
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