A kind of flavonoid compound and its preparation method and application

A technology of flavonoids and plants, applied in organic chemistry, sugar derivatives, antiviral agents, etc., can solve the problem of lack of safe and effective control methods for influenza virus

Inactive Publication Date: 2021-07-02
YUNNAN MINZU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But as of now, the existing human medical level has always lacked a safe and effective control method for influenza virus

Method used

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  • A kind of flavonoid compound and its preparation method and application
  • A kind of flavonoid compound and its preparation method and application
  • A kind of flavonoid compound and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Take 12kg of dried branches, leaves and / or fruits of leguminous trees, coarsely crush them to 20 meshes, and ultrasonically extract them with 90% ethanol water for 4 times, each time for 30 minutes, and combine the extracts; filter the extracts, and concentrate under reduced pressure to volume 1 / 4; stand still, filter out the precipitate, and concentrate to 2767g extract a; add 2760g water to extract a, extract 5 times with chloroform equal to the volume of water, combine the extract phases, and concentrate under reduced pressure to obtain 1430g extract b; use 14000g of 200 mesh silica gel to pack the column, add 1000g of methanol to the extract b to dissolve, then add 1400g of 100 mesh silica gel to mix the sample, and put the sample on the column after mixing; the volume ratio is 1:0, 20:1, 9 respectively :1, 8:2, 7:3, 3:2, 1:1, 1:2, 0:1 dichloromethane-methanol mixed organic solvent gradient elution, the gradient eluate was collected, concentrated, and monitored by TL...

Embodiment 2

[0038] Take 4.8kg of dried branches, leaves and / or fruits of leguminous trees, coarsely crush them to 20 mesh, and extract them three times with 100% ethanol ultrasonically, each time for 30 minutes, and combine the extracts; filter the extracts, and concentrate under reduced pressure to volume 1 / 3; stand still, filter out the precipitate, and concentrate into 560g extract a; add 560g water to extract a, extract 3 times with chloroform equal to the volume of water, combine the extract phases, concentrate under reduced pressure to obtain 320g extract a Extract b; use 4000g of 200-300 mesh silica gel to pack the column, add 340g of ethyl acetate to the extract b to dissolve, then add 320g of 100 mesh silica gel to mix the sample, and put the sample on the column after mixing; use volume ratios of 20:1, 9:1, 8:2, 7:3, 6:4, 1:1, 1:2, 0:1 dichloromethane-ethyl acetate mixed organic solvent gradient elution, collect the gradient eluate, concentrate, After TLC monitoring, the same pa...

Embodiment 3

[0040]Take 6.5kg of dried branches, leaves and / or fruits of leguminous arbor, coarsely crush them to 30 meshes, and ultrasonically extract them with 80% methanol for 4 times, each time for 30 minutes, and combine the extracts; filter the extracts, concentrate under reduced pressure to volume 1 / 2; stand still, filter out the precipitate, and concentrate into 675g extract a; add 700g water to extract a, extract 4 times with diethyl ether equal to the volume of water, combine the extract phases, concentrate under reduced pressure to obtain 342g extract a Extract b; use 2800g of 180 mesh silica gel to pack the column, add 930g of acetone to the extract b to dissolve, then add 360g of 90 mesh silica gel to mix the sample, and put the sample on the column after mixing; the volume ratio is 1:0, 20:1, 9:1, 8:2, 7:3, 3:2, 1:1, 1:2, 0:1 chloroform-acetone mixed organic solvent gradient elution, the gradient eluate was collected, concentrated, and monitored by TLC. Combine the same parts...

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Abstract

The invention discloses a flavonoid compound and its preparation method and application. The molecular formula of the flavonoid compound is C 24 h 24 o 10 , the compound is called kaempferol-3-O-[(4'',5''-O-isopropylidene)-a-L-rhamnopyranoside], which has the following structural formula: the preparation method of the flavonoids is to dry The branch, leaf or fruit of the arbor plant of the genus Fabaceae Cassia is used as raw materials, and it is obtained through the separation steps of extract extract, organic solvent extraction, silica gel column chromatography, and high-pressure liquid chromatography. The application is the application of flavonoids in neuraminidase inhibitory activity. In the neuraminidase inhibitory activity experiment, oseltamivir carboxylate was selected as a control, and kaempferol‑3‑O‑[(4′′,5′′‑O‑isopropylidene)‑a‑L‑rhamnopyranoside] was used as a control for neuraminidase acidase IC 50 The value is 187.40 mu mol / L, which has good neuraminidase inhibitory activity. The compound of the invention has simple structure and good activity, can be used as a leading compound of anti-influenza virus medicine, and has good application prospect.

Description

technical field [0001] The invention belongs to the technical field of extraction of effective plant components, and in particular relates to a flavonoid compound and its preparation method and application. Background technique [0002] Fabaceae Sumacinae Cassia ( Cassia L .) There are more than 600 species of plants in the world, which are widely distributed in tropical and subtropical regions of the earth, especially in tropical America and tropical Africa. There are 25 species in my country, which are widely distributed in the southern provinces, and all species are distributed in Yunnan. The main chemical constituents of Cassia plants are triterpenes, anthraquinones, steroids, alkaloids, flavonoids, stilbenes and other types; these compounds exhibit anti-cancer, anti-HIV virus, anti-hepatitis, insecticide, anxiolytic and sedative properties and other activities. In recent years, domestic researchers have discovered active new compounds from Cassia Dai medicine plants:...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/07A61P31/16
CPCA61P31/16C07H17/07
Inventor 高雪梅朱鸿王闪闪张再崔笛
Owner YUNNAN MINZU UNIV
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