Kit for quantitatively detecting ovarian cancer exosome antigen and preparation method thereof

A technology for quantitative detection and preparation methods, which is applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of long time, large loss of exosomes, and many reagents, and achieves short reaction period, strong specificity, and wide range. Effect

Inactive Publication Date: 2019-01-29
上海领潮生物新材料有限公司
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0005] Nanoparticle tracking analysis is a light scattering technique that can characterize the distribution of nanoparticles. For nanoparticles suspended in liquid, the Brownian motion is directly related to the size. According to this principle, the Brownian motion of each particle can be tracked in real time and Given the particle size and number information, since the particles in the biological samples of exosomes are dispersed, the subtypes of exosomes cannot be distinguished by nanoparticle tracking analysis;
[0006] Flow cytometry is a technology that uses a laser beam to excite cells that flow in a single line, and detects its scattered l

Method used

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  • Kit for quantitatively detecting ovarian cancer exosome antigen and preparation method thereof
  • Kit for quantitatively detecting ovarian cancer exosome antigen and preparation method thereof

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Embodiment 1

[0048] 1. Prepare the kit according to the following method:

[0049] (1), the preparation of the ELISA plate coated with the clonal antibody against CD63 adopts the following steps:

[0050] a1. Take a 96T blank ELISA plate, coat each well with 100 μl of anti-CD63 monoclonal antibody, incubate overnight at 4°C, and remove the supernatant;

[0051] a2. Add 200 μl of blocking solution per well, the blocking solution is 10wt% bovine serum and 90wt% 10mM PBS, place in a constant temperature incubator at 37°C for 2 hours, repeat washing several times, and pat dry;

[0052] a3. After the sealing is completed, shake out the sealing solution, pat the board dry on absorbent paper, and set aside;

[0053] (2), the preparation of the elisa plate coated with the clonal antibody against CA125 adopts the following steps:

[0054] b1. Take another 96T blank microtiter plate, coat each well with 100 μl of anti-CA125 monoclonal antibody, incubate overnight at 4°C, and remove the supernatant; ...

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Abstract

The invention relates to the technical field of ELISA detection kits, and particularly discloses a high-capacity asphalt/epoxy resin-based hard carbon negative electrode material and a preparation method of same. The kit includes a box body, in which two ELISA plates coated with anti-CD63 cloning antibody, an ELISA plate coated with anti-CA125 cloning antibody, 10 ml of HPR-labeled CA125 antibody,10 ml of HPR-labeled CD63 antibody, 20 ml of H2O2 being a color developing agent A solution, 20 ml of TMB being a color developing agent B solution, and two bottles of 5 ml of stop solution. Comparedwith the prior art, by means of the exosome ELISA method, an antigen of the ovarian cancer is quickly detected, thus completing early diagnosis on the ovarian cancer. The kit is high in detection accuracy and specificity and is extensive in detectable sample range, which may include serum, ascites, blood plasma, tissue liquid and the like. The kit is short in reaction period and has good effect.

Description

technical field [0001] The invention relates to the technical field of ELISA detection kits, in particular to a high-capacity asphalt / epoxy resin-based modified hard carbon negative electrode material and a preparation method thereof. Background technique [0002] Ovarian cancer is the third most common gynecological malignancy after endometrial cancer and cervical cancer, and its mortality rate ranks first among gynecological malignancies. Ovarian cancer has an insidious onset and lacks early typical symptoms and mature early diagnosis methods. When clinical symptoms appear and patients pay attention to seek medical treatment, it is often at an advanced stage. When extensive abdominal metastases appear, radical surgery is difficult to implement. [0003] Exosomes exist in various body fluids and can serve as carriers of intercellular information transmission, playing an important role in cell communication, especially in the field of disease marker screening. Exosomes (exo...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/558G01N33/577G01N33/535
CPCG01N33/535G01N33/558G01N33/57449G01N33/577
Inventor 罗朝领茅柳娟王莹曹雪姣任调
Owner 上海领潮生物新材料有限公司
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