Kit for quantitatively detecting ovarian cancer exosome antigen and preparation method thereof

Abstract

The invention relates to the technical field of ELISA detection kits, and particularly discloses a high-capacity asphalt / epoxy resin-based hard carbon negative electrode material and a preparation method of same. The kit includes a box body, in which two ELISA plates coated with anti-CD63 cloning antibody, an ELISA plate coated with anti-CA125 cloning antibody, 10 ml of HPR-labeled CA125 antibody,10 ml of HPR-labeled CD63 antibody, 20 ml of H2O2 being a color developing agent A solution, 20 ml of TMB being a color developing agent B solution, and two bottles of 5 ml of stop solution. Comparedwith the prior art, by means of the exosome ELISA method, an antigen of the ovarian cancer is quickly detected, thus completing early diagnosis on the ovarian cancer. The kit is high in detection accuracy and specificity and is extensive in detectable sample range, which may include serum, ascites, blood plasma, tissue liquid and the like. The kit is short in reaction period and has good effect.

Description

technical field [0001] The invention relates to the technical field of ELISA detection kits, in particular to a high-capacity asphalt / epoxy resin-based modified hard carbon negative electrode material and a preparation method thereof. Background technique [0002] Ovarian cancer is the third most common gynecological malignancy after endometrial cancer and cervical cancer, and its mortality rate ranks first among gynecological malignancies. Ovarian cancer has an insidious onset and lacks early typical symptoms and mature early diagnosis methods. When clinical symptoms appear and patients pay attention to seek medical treatment, it is often at an advanced stage. When extensive abdominal metastases appear, radical surgery is difficult to implement. [0003] Exosomes exist in various body fluids and can serve as carriers of intercellular information transmission, playing an important role in cell communication, especially in the field of disease marker screening. Exosomes (exo...

AI Technical Summary

Problems solved by technology

[0005] Nanoparticle tracking analysis is a light scattering technique that can characterize the distribution of nanoparticles. For nanoparticles suspended in liquid, the Brownian motion is directly related to the size. According to this principle, the Brownian motion of each particle can be tracked in real time and Given the particle size and number information, since the particles in the biological samples of exosomes are dispersed, the subtypes of exosomes cannot be distinguished by nanoparticle tracking analysis;

[0006] Flow cytometry is a technology that uses a laser beam to excite cells that flow in a single line, and detects its scattered light and fluorescence to complete cell analysis and sorting. However, due to the small volume of exosome microbubbles, Conventional streaming methods are not easy to detect;

[0007] Dynamic light scattering is an effective technique to obtain the particle size of the particles to be measured mixed in it by studying the fluctuation of scattered light intensity at a fixed spatial position with time, but it is not suitable for exosome samples due to its complexity. Measurement of exosomes of various sizes

Too many reagents are consumed in the process of exosome isolation and detection technology, and the loss of exosomes is large and the time is long

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