Kit applied to detecting apoA I and apo B and preparation method of stabilizer of kit

Abstract

The invention relates to a kit applied to detecting apoA I and apo B and a preparation method of a stabilizer of the kit. The kit comprises apo A antibody liquid, apo B antibody liquid and liquid serotype constant value calibration solution, and also comprises the stabilizer; the stabilizer comprises the following components in parts by weight: 1-5 parts of trehalose, 100-200 parts of 0.01mol/L phosphate buffer, 1-5 parts of horseradish peroxidase, 0.01-0.1 part of sodium propionate and 1-5 parts of thiomersalate. By using the stabilizer, the stability of the antibodies is enhanced, and thus the preservation life and the service life are prolonged, the stability of the kit when in use is enhanced, and the trehalose can protect the stability of biomacromolecule structure and functions whenliving bodies are coerced by bad environment conditions.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a preparation method of a kit for detecting apoA I and apoB and a stabilizer thereof. Background technique [0002] Apolipoprotein A I (apo A I) is the most abundant component of the apoA group. ApoA I is a single polypeptide chain consisting of 243 amino acid residues with a molecular weight of 28300D. It is the main apolipoprotein of high-density lipoprotein (HDL) . HDL has routine detection items to prevent cardiovascular and cerebrovascular diseases. Apolipoprotein B (apoB) is the main protein of low-density lipoprotein (LDL), and increased LDL is a recognized risk factor for atherosclerosis. Therefore, the determination of serum apoB plays a very important role in clinical practice. The detection of apo A I and apoB provides a reliable basis for early diagnosis, early prevention, and early treatment of cardiovascular and cerebrovascular diseases. [0003] Known ...

AI Technical Summary

Problems solved by technology

[0003] Known methods for measuring apo A I and apoB include chromatography, electrophoresis, and immunoassay. Among them, chromatography and electrophoresis are cumbersome to operate, and they cannot perform batch sample analysis and directly go to full-automatic biochemical analyzers. The immunodiffusion method, radioimmunoassay method, fluorescent labeling immunoassay method, enzyme-labeled immunoassay method, and chemiluminescence immunoassay method also have many deficiencies. If special equipment is required, the sample needs to be pretreated, and it cannot be used on a global scale. Automatic biochemical analyzer for batch detection and analysis, etc.

The immunoturbidimetric method commonly used in clinical practice is popular because of its small amount of sample, which can be directly analyzed in batches on an automatic biochemical analyzer, and is easy to operate. However, there are some or insufficient methods and reagents established at present. In: First, the antibody titer is not high, all below 1:16, and the specificity, affinity, and affinity are not high. Second, the calibration serum is based on human serum. Human serum with normal acid aminotransferase, but it is difficult to rule out the possibility of no other infectious diseases, which brings great danger to the operator, and it is a freeze-dried product, the coal belt has different values, and water is required before use Reconstitution is extremely inconvenient to operate, and there are large differences between reconstituted bottles after reconstitution. After reconstitution, it must be frozen and stored, especially if it cannot be thawed repeatedly, which not only causes waste of reagents, but also the quality cannot be guaranteed. The results vary greatly, and its stability is not good; the third is the poor anti-interference ability for high-fat, jaundice, and hemolysis samples

Fourth, the single-point calibration solution is used, which does not conform to the calculation of the curve regression equation, resulting in the phenomenon of low high values ​​and high low values, and the measured results are inaccurate

Fifth, the antiserum precipitates in a short period of time, making it difficult to operate on the machine and affecting the detection effect

The stabilizer in this solution uses high-concentration animal serum as the stabilizer, but its protective effect is not ideal, and it will lose effect after being stored for a period of time, so it needs to be improved

Images