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Preparation method and application of a multi-stage delivery nanoparticle capable of tumor-targeted delivery of nucleic acid drugs

A technology of nanoparticles and nucleic acid drugs is applied in the field of preparation of multi-stage delivery nanoparticles, and achieves the effects of easy popularization and application, efficient delivery and low cost

Active Publication Date: 2020-12-22
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these different stages require the delivery system to have completely different surface properties, so integrating these three requirements into one system is a great challenge

Method used

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  • Preparation method and application of a multi-stage delivery nanoparticle capable of tumor-targeted delivery of nucleic acid drugs
  • Preparation method and application of a multi-stage delivery nanoparticle capable of tumor-targeted delivery of nucleic acid drugs
  • Preparation method and application of a multi-stage delivery nanoparticle capable of tumor-targeted delivery of nucleic acid drugs

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Example 1: A method for preparing multistage delivery nanoparticles capable of tumor-targeted delivery of nucleic acid drugs.

[0027] attached figure 1 A schematic diagram of the preparation and application of the multi-stage delivery nanoparticles of the present invention is shown. The multi-stage delivery nanoparticles prepared by the method of the present invention are spherical particles with a particle diameter of 150±30 nm and uniformly distributed core-shell structure. The nanoparticle is firstly composed of a positively charged nanocomposite inner core formed by phenylboronic acid-modified low-molecular-weight polyethyleneimine and nucleic acid, and then formed by electrostatic self-assembly with an environment-responsive anionic polymer shell. See attached figure 1 , a method for preparing multistage delivery nanoparticles capable of achieving tumor-targeted delivery of nucleic acid drugs, comprising the following steps:

[0028] 1) Modification of low molec...

Embodiment 2

[0038] Example 2: A method for preparing multi-stage delivery nanoparticles capable of tumor-targeted delivery of nucleic acid drugs.

[0039] attached figure 1 A schematic diagram of the preparation and application of the multi-stage delivery nanoparticles of the present invention is shown. The multi-stage delivery nanoparticles prepared by the method of the present invention are spherical particles with a particle diameter of 150±30 nm and uniformly distributed core-shell structure. The nanoparticle is firstly composed of a positively charged nanocomposite inner core formed by phenylboronic acid-modified low-molecular-weight polyethyleneimine and nucleic acid, and then formed by electrostatic self-assembly with an environment-responsive anionic polymer shell. See attached figure 1 , a method for preparing multistage delivery nanoparticles capable of achieving tumor-targeted delivery of nucleic acid drugs, comprising the following steps:

[0040] 1) Modification of low mole...

Embodiment 3

[0050] Example 3: A method for preparing multi-stage delivery nanoparticles capable of tumor-targeted delivery of nucleic acid drugs.

[0051] attached figure 1 A schematic diagram of the preparation and application of the multi-stage delivery nanoparticles of the present invention is shown. The multi-stage delivery nanoparticles prepared by the method of the present invention are spherical particles with a particle diameter of 150±30 nm and uniformly distributed core-shell structure. The nanoparticle is firstly composed of a positively charged nanocomposite inner core formed by phenylboronic acid-modified low-molecular-weight polyethyleneimine and nucleic acid, and then formed by electrostatic self-assembly with an environment-responsive anionic polymer shell. See attached figure 1 , a method for preparing multistage delivery nanoparticles capable of achieving tumor-targeted delivery of nucleic acid drugs, comprising the following steps:

[0052] 1) Modification of low mole...

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Abstract

The invention relates to a preparation method and applications of multi-stage delivery nanoparticles capable of achieving tumor-targeted delivery of nucleic acid drugs. According to the present invention, the multi-stage delivery nanoparticles have a core-shell structure, wherein the multi-stage delivery nanoparticles can present different surface characteristics based on the surrounding environment through the environmentally responsive shell layer, such that the multi-stage delivery nanoparticles can overcome multiple physiological barriers and efficiently deliver nucleic acid drugs to tumortissues; and with the injection of the multi-stage delivery nanoparticles carrying fluorescent probe-labeled plasmid DNA into tumor-bearing mice, the nucleic acid drug can be efficiently enriched inthe tumor tissue, such that the results show that the tumor-targeted delivery of nucleic acid drugs can be completely achieved by using the multi-stage delivery nanoparticles.

Description

technical field [0001] The invention belongs to the field of macromolecule biomaterials, and relates to a preparation method and application of multi-stage delivery nanoparticles capable of realizing tumor-targeted delivery of nucleic acid drugs. Background technique [0002] Advances in genetics and molecular biology research have shown that the occurrence and development of cancer are related to a variety of genetic changes and disorders. Gene therapy can deliver therapeutic nucleic acid drugs to cancer cells to correct or modify genetic information, showing great potential for cancer treatment at the genetic level. Gene therapy for cancer includes suicide gene therapy, silencing of oncogene expression, mutation correction, enhanced tumor suppression, suppression of tumor angiogenesis and stimulation of immune responses against tumor cells. In the past 25 years, more than 2,000 clinical trials of gene therapy have been conducted, about two-thirds of which are used to trea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/51A61K47/32A61K47/34A61K31/711A61P35/00B82Y5/00
CPCA61K9/5138A61K9/5146A61K31/711A61P35/00B82Y5/00
Inventor 刘阳刘琦康春生赵凯
Owner NANKAI UNIV