Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Nitric oxide and cis-platinum targeting combined controllable administration nano drug system and preparation

A nano-carrier and drug technology, applied in the field of multifunctional bimetallic nanocomposite drug system and its preparation

Active Publication Date: 2019-02-01
EAST CHINA UNIV OF SCI & TECH
View PDF1 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Based on the above analysis, how to selectively deliver the extremely unstable small molecule of NO to specific cells or subcellular organelles at an appropriate concentration, combine NO with other chemotherapy drugs, and improve the anticancer effect of the drug through controllable combined administration Efficacy is yet to be studied

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nitric oxide and cis-platinum targeting combined controllable administration nano drug system and preparation
  • Nitric oxide and cis-platinum targeting combined controllable administration nano drug system and preparation
  • Nitric oxide and cis-platinum targeting combined controllable administration nano drug system and preparation

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0141] The present invention also provides a preparation method of the pharmaceutical system of the present invention, which generally includes the following steps:

[0142] (1) Provide metal nitrosyl NO donors, platinum complexes, targeting groups and carrier nanoparticles;

[0143] (2) covalently loading the metal nitrosyl NO donor, the platinum complex, the targeting group and the carrier nanoparticles to form the bimetallic nanocomposite drug system .

[0144] In another preferred example, in step (1), the metal nitrosyl NO donor includes a metal nitrosyl compound (A)[(tpy')M 1 (R 1 )(NO)](PF 6 ) 3 , tpy' is 4'-formic acid-2,2':6',2"-terpyridine, M 1 For metal ruthenium (Ru), R 1 For 3-formic acid-o-phenylenediamine or its derivatives.

[0145] In another preferred example, in step (1), the platinum complex includes metal compound (B) cis-[M 2 (NH 3 ) 2 Cl 2 (OH) 2 ], M 2 For the metal platinum (Pt).

[0146] In another preferred example, the carrier nanopart...

Embodiment 1

[0159] Metal Ru nitrosyl compounds [(tpy COOH )Ru(MDAB)(NO)](PF 6 ) 3 Synthesis

[0160] (1)[(tpy COOH )Ru(MDAB)(Cl)](PF 6 )Synthesis

[0161] (tpy COOH is 4'-formic acid-2,2':6',2"-terpyridine; MDAB is methyl 3,4-diaminobenzoate):

[0162] Add Ru(tpy COOH ) Cl 3 (150mg, 0.31mmol), MDAB, (55mg, 0.33mmol), LiCl (5mg, 2.0mmol) and Et 3 N 0.4mL, vacuumize and nitrogen three times respectively, add 40mL of EtOH / H 2 O(3:1, v / v), in N 2 Reflux in the atmosphere for 8 hours, suction filter while hot, and concentrate the dark red filtrate to several milliliters. After cooling to room temperature, add excess saturated NH 4 PF 6 solution, and the mixture was placed in a refrigerator at 5°C overnight. The reddish-brown precipitate was filtered out and washed with H 2 O and Et 2 O were washed three times, respectively, and dried in vacuo. 137 mg of the target product was obtained with a yield of 61%.

[0163] (2)[(tpy COOH )Ru(MDAB)(NO 2 )](PF 6 )Synthesis:

[0164] W...

Embodiment 2

[0169] Synthesis of Nanoparticle Composite Drug System {N-GQDs@Ru-NO-Pt@FA}

[0170] (1) N-GQDs@NH 2 (Surface Aminated N-GQDs Nanoparticles) Preparation

[0171] Weigh citric acid (40 mg), dissolve it in 40 mL of distilled water, add ammonia water (8 mL), and place the solution in a muffle furnace at 200° C. for 3 h. After cooling to room temperature, the pH was adjusted to 8 to obtain a light yellow solution, which was dialyzed in water for 4 h with a dialysis bag with a molecular weight cut-off of 1000, and the solution was rotary evaporated to obtain N-GQDs as a solid. Dissolve N-GQDs in 2.0 mL of distilled water, add EDC / NHS, activate for 1 h, add 1 mL of anhydrous ethylenediamine, and stir at room temperature for 24 h. The solution was dialyzed in water for 24 hours with a dialysis bag with a molecular weight cut-off of 1000, and the solution in the dialysis bag was freeze-dried to obtain the product N-GQDs@NH 2 .

[0172] (2) Preparation of nanoparticle composite dru...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Light intensityaaaaaaaaaa
Login to View More

Abstract

The invention provides a nitric oxide and cis-platinum targeting combined controllable administration nano drug system. The nitric oxide and cis-platinum targeting combined controllable administrationnano drug system structurally comprises a vector, an exogenous NO donor and a metal complex, wherein the vector is a nano vector; the exogenous NO donor is ruthenium nitrosyl NO donor; and the metalcomplex is a platinum complex. The drug system has a fluoresent tracer, can selectively target specific cancel cells, can deliver nitric oxide and cis-platinum drugs under the control of near-infraredlight, and meanwhile has a photothermal therapy function. An efficient anti-cancer effect is achieved by cooperation of nitric oxide and cis-platinum combined administration and the photothermal therapy method.

Description

technical field [0001] The invention relates to the field of biology and the field of inorganic nano medicines. Specifically, the present invention provides a multifunctional bimetallic nanocomposite drug system with fluorescent tracer, targeted delivery, and intracellular near-infrared light photocontrol delivery of nitric oxide and cisplatin, and a preparation method thereof. Background technique [0002] Cis-dichlorodiammine platinum (II) (cisplatin, cisplatin) has been widely used in the chemotherapy of various cancers since it was discovered in the 1970s that it can inhibit the growth of tumor cells, and it plays an important role in chemotherapy drugs. Location. Mechanism of action of platinum: After entering the body, it acts on tumor cell DNA, coordinates with DNA to form an adduct, and inhibits DNA replication, thereby causing tumor cell apoptosis. [0003] Cisplatin is a first-line chemotherapy drug for several cancers, including ovarian, breast, bladder, head an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K47/52A61K47/69A61K49/00A61K41/00A61K33/00A61K33/243A61P35/00
CPCA61K41/0052A61K47/52A61K47/6923A61K49/0067A61P35/00A61K33/00A61K33/24A61K2300/00
Inventor 刘劲刚史淑文李延慧喻雅婷
Owner EAST CHINA UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com